JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313468

Anti-DR3/LARD antibody [RP23040174]

Be the first to review this product! Submit a review

|

(0 Publication)

Anti-Anti antibody [RP23040174] is a Rabbit Recombinant Multiclonal antibody that is used in Anti ICC/IF, Western Blot. Suitable for Human, Mouse, Rat samples.

Death Receptor 3 (DR3), also known as TNFRSF25, is a member of the tumor necrosis factor receptor superfamily. It plays a significant role in cancer through its interactions with its ligand, TNF-like protein 1A (TL1A). DR3 is expressed by various immune cells, including innate lymphoid cells (ILCs) and T cells. Its activation by TL1A can lead to the expansion and activation of these cells, which are crucial for immune responses against tumors.DR3 mediates apoptotic signaling, which can lead to the programmed cell death of cancer cells. This mechanism is vital for controlling tumor growth and progression.

View Alternative Names

APO3, DDR3, DR3, TNFRSF12, WSL, WSL1, UNQ455/PRO779, TNFRSF25, Tumor necrosis factor receptor superfamily member 25, Apo-3, Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor DR3, Apoptosis-mediating receptor TRAMP, Death receptor 3, Lymphocyte-associated receptor of death, Protein WSL, Protein WSL-1, LARD

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-DR3/LARD Antibody [RP23040174] (AB313468)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DR3/LARD Antibody [RP23040174] (AB313468)

Immunofluorescence was performed on fixed and permeabilized K562 cells for detection of endogenous DR3/TNFRSF25 using ab313468 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1 : 2000). Panel a) shows representative cells that were stained for detection and localization of DR3/TNFRSF25 protein (green), Panel b) is stained for nuclei (blue) using with DAPI . Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin ( 1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of DR3/TNFRSF25. Panel e) represents control cells with no primary antibody to assess background.

Western blot - Anti-DR3/LARD Antibody [RP23040174] (AB313468)
  • WB

Supplier Data

Western blot - Anti-DR3/LARD Antibody [RP23040174] (AB313468)

A 45kDa band along with 13 kDa isoform corresponding to DR3/TNFRSF25 was observed across cell lines tested. Known quantity of protein samples were electrophoresed 4-12% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk.

All lanes:

Western blot - Anti-DR3/LARD Antibody [RP23040174] (ab313468) at 2 µg/mL

Lane 1:

Whole cell extracts of K-562 at 30 µg

Lane 2:

Whole cell extracts of Jurkat at 30 µg

Lane 3:

Whole cell extracts of KARPAS 299 at 30 µg

Lane 4:

Whole cell extracts of THP-1 at 30 µg

Lane 5:

Whole cell extracts of PC-3 at 30 µg

Lane 6:

Whole cell extracts of SH-SY5Y at 30 µg

Lane 7:

Whole cell extracts of HeLa at 30 µg

Lane 8:

Whole cell extracts of Caco-2 at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040174

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TNFRSF25.

Q93038

style
left-column

Complementary Products

Primary Antibodies

AB274358

Anti-TL1A antibody [EPR23330-106] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-TL1A antibody [EPR23330-106] - BSA and Azide free (Capture) (AB274358)

  • 0
  • 0
Primary Antibodies

AB108309

Anti-CD1a antibody [EP3622]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] (AB108309)

  • 0
  • 0
Primary Antibodies

AB225636

Anti-HLA Class 1 ABC antibody [EPR22172]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (AB225636)

  • 4
  • 1
Primary Antibodies

AB254257

Anti-POMC antibody [EPR22534-165]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] (AB254257)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DR3 receptor also known as LARD (Lymphocyte Apoptosis-Related Receptor Domain) is a member of the TNF receptor superfamily. This protein also referred to as the DR3 protein or LARD protein is involved in mediating apoptotic signals. DR3 is expressed predominantly in lymphoid tissues and plays an important role in immune system regulation. DR3 protein has an approximate mass of 65 kDa. Scientists study DR3 in various models including cyno RNA and protein monkey models to gain better understanding of its function and expression.
Biological function summary

DR3 functions significantly in the immune response by modulating apoptosis. It forms part of a complex with other proteins leading to the activation of signaling pathways that result in cell death. The binding of its ligand TL1A to DR3 initiates a series of downstream effects that influence T-cell and other immune cell activities. LARD target has a functional correlation with the regulation of immune response and the maintenance of homeostasis in immune cell populations.

Pathways

DR3 receptor mediates signals primarily in two significant pathways: the NF-kB pathway and the MAPK pathway. These pathways play a role in transducing signals that lead to inflammation and immune responses. DR3 interacts with other proteins such as TRADD and RIPK1 facilitating the propagation of signals through these pathways. The engagement of DR3 in these pathways highlights its regulatory influence over immune cell survival and function.

DR3 plays an important role in autoimmune diseases and inflammatory disorders. Dysregulation of DR3 receptor and its signaling pathways can contribute to conditions like rheumatoid arthritis and inflammatory bowel disease. There is a notable connection between dysfunction in DR3 signaling and altered interactions with pro-inflammatory proteins such as TNF-alpha which can exacerbate these conditions. Understanding DR3's role helps in developing therapeutic approaches to manage these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.
See full target information TNFRSF25
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com