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AB14738

Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free

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(2 Publications)

Mouse Monoclonal DR4 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF10A.

View Alternative Names

CD261, APO2, DR4, TRAILR1, TNFRSF10A, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1

2 Images
Flow Cytometry - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (AB14738)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (AB14738)

Flow Cytometry analysis of DR4 expression on the surface of hematopoietic cell lines. Cells were stained with purified anti-DR4 antibodies (ab14738) followed by Goat anti-mouse IgG PE. Histograms : Red - Goat anti-mouse IgG PE (no primary antibody) Black - anti-DR4 comparative antibody Blue and Green - anti-DR4 (ab14738 : DR-4-02) in duplicate staining

Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (AB14738)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (AB14738)

Immunofluorescence staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with DR4 expression plasmid using anti-human DR4 (ab14738 : DR-4-02).

  • 660 APC

    APC Anti-DR4 antibody [DR-4-02]

  • 519 FITC

    FITC Anti-DR4 antibody [DR-4-02]

  • 578 PE

    PE Anti-DR4 antibody [DR-4-02]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DR-4-02

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human TNFRSF10A. The exact immunogen used to generate this antibody is proprietary information.

O00220

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Alternative Products

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB73400

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Primary Antibodies

AB77528

Anti-YKL-40/CHI3L1 antibody

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  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DR4 also known as TRAIL-R1 is a receptor with a molecular weight of approximately 60 kDa. It is part of the tumor necrosis factor receptor (TNFR) superfamily. DR4 is widely expressed in various types of tissues such as the liver spleen thymus and peripheral blood leukocytes. It functions mechanically by binding to its ligand TNF-related apoptosis-inducing ligand (TRAIL) which initiates signaling cascades that lead to apoptosis or programmed cell death.
Biological function summary

The death receptor 4 plays an important role in mediating cell apoptosis. Upon ligand binding DR4 interacts with the adapter molecule FADD (FAS-associated protein with death domain) facilitating the formation of the death-inducing signaling complex (DISC). This complex recruits and activates caspase-8 which eventually leads to the activation of the downstream effector caspases driving the cell towards apoptosis. DR4 operates as a significant component of the extrinsic pathway of apoptosis a process important for maintaining cellular homeostasis and eliminating harmful cells.

Pathways

DR4 functions critically within the extrinsic apoptotic pathway. This pathway involves other death receptors such as DR5 which also binds TRAIL similarly leading to apoptosis through DISC formation and caspase activation. The extrinsic pathway interconnects with the intrinsic pathway via the mitochondria with proteins like Bid acting as a bridge between them. The engagement of these pathways highlights DR4’s importance in regulatory mechanisms of cell death tightly controlling cellular proliferation and survival.

DR4 plays a significant role in cancer and autoimmune diseases. Alterations in DR4 expression or function can lead to evasion of apoptosis by cancer cells contributing to tumor progression. The defective apoptotic signaling via DR4 is also linked to autoimmune disorders where insufficient apoptosis may allow for the survival of autoreactive immune cells. In both scenarios DR4's involvement usually associates with its counterpart DR5 as they share similar ligand-binding properties and signal transduction mechanisms in diseased states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed : 26457518, PubMed : 38532423). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PubMed : 19090789). Promotes the activation of NF-kappa-B (PubMed : 9430227).
See full target information TNFRSF10A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 20:1521-31 PubMed23933815

2013

Systems analysis of apoptosis protein expression allows the case-specific prediction of cell death responsiveness of melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

E Passante,M L Würstle,C T Hellwig,M Leverkus,M Rehm

Molecular cancer research : MCR 7:1045-55 PubMed19584265

2009

Matrix protein CCN1 is critical for prostate carcinoma cell proliferation and TRAIL-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Carrie A Franzen,Chih-Chiun Chen,Viktor Todorović,Vladislava Juric,Ricardo I Monzon,Lester F Lau
View all publications
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Product promise

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