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Knockout Tested Rabbit Recombinant Monoclonal DR4 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-DR4 antibody [EPR28152-57] (AB312848), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [EPR28152-57] (AB312848), expandable thumbnail
  • Western blot - Anti-DR4 antibody [EPR28152-57] (AB312848), expandable thumbnail
  • Western blot - Anti-DR4 antibody [EPR28152-57] (AB312848), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)IHC-PIP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:26457518, PubMed:38532423). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PubMed:19090789). Promotes the activation of NF-kappa-B (PubMed:9430227).

Alternative names

CD261, APO2, DR4, TRAILR1, TNFRSF10A, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal DR4 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28152-57
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DR4 also known as TRAIL-R1 is a receptor with a molecular weight of approximately 60 kDa. It is part of the tumor necrosis factor receptor (TNFR) superfamily. DR4 is widely expressed in various types of tissues such as the liver spleen thymus and peripheral blood leukocytes. It functions mechanically by binding to its ligand TNF-related apoptosis-inducing ligand (TRAIL) which initiates signaling cascades that lead to apoptosis or programmed cell death.

Biological function summary

The death receptor 4 plays an important role in mediating cell apoptosis. Upon ligand binding DR4 interacts with the adapter molecule FADD (FAS-associated protein with death domain) facilitating the formation of the death-inducing signaling complex (DISC). This complex recruits and activates caspase-8 which eventually leads to the activation of the downstream effector caspases driving the cell towards apoptosis. DR4 operates as a significant component of the extrinsic pathway of apoptosis a process important for maintaining cellular homeostasis and eliminating harmful cells.

Pathways

DR4 functions critically within the extrinsic apoptotic pathway. This pathway involves other death receptors such as DR5 which also binds TRAIL similarly leading to apoptosis through DISC formation and caspase activation. The extrinsic pathway interconnects with the intrinsic pathway via the mitochondria with proteins like Bid acting as a bridge between them. The engagement of these pathways highlights DR4’s importance in regulatory mechanisms of cell death tightly controlling cellular proliferation and survival.

Associated diseases and disorders

DR4 plays a significant role in cancer and autoimmune diseases. Alterations in DR4 expression or function can lead to evasion of apoptosis by cancer cells contributing to tumor progression. The defective apoptotic signaling via DR4 is also linked to autoimmune disorders where insufficient apoptosis may allow for the survival of autoreactive immune cells. In both scenarios DR4's involvement usually associates with its counterpart DR5 as they share similar ligand-binding properties and signal transduction mechanisms in diseased states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry (Intracellular) - Anti-DR4 antibody [EPR28152-57] (ab312848), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-DR4 antibody [EPR28152-57] (ab312848)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized TNFRSF10A (DR4) knockout HeLa line (Left) / Parental HeLa cell line (Right) cells labelling DR4 with ab312848 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [EPR28152-57] (ab312848), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [EPR28152-57] (ab312848)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling DR4 with ab312848 at 1/50 (9.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in HCT 116 cell line. Negative control: 293T. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Western blot - Anti-DR4 antibody [EPR28152-57] (ab312848), expandable thumbnail

    Western blot - Anti-DR4 antibody [EPR28152-57] (ab312848)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Performed under reducing conditions.
    In Western blot, ab312848 was shown to bind specifically to TNFRSF10A (DR4). A band was observed at 45 kDa in wild-type Hela cell lysates whereas loss of signal was observed in the TNFRSF10A (DR4) knockout cell line Human TNFRSF10A (DR4) knockout HeLa cell line ab264687 (knockout cell lysate Human TNFRSF10A (DR4) knockout HeLa cell lysate ab258726).
    In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-DR4 antibody [EPR28152-57] (ab312848) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 2: Western blot - Human TNFRSF10A (DR4) knockout HeLa cell lysate (Human TNFRSF10A (DR4) knockout HeLa cell lysate ab258726) at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 45 kDa

    Exposure time: 15s

  • Western blot - Anti-DR4 antibody [EPR28152-57] (ab312848), expandable thumbnail

    Western blot - Anti-DR4 antibody [EPR28152-57] (ab312848)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: 293T, Fadu.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-DR4 antibody [EPR28152-57] (ab312848) at 1/1000 dilution

    Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 2: Fadu (human pharyeal carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 45 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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