Knockout Tested Rabbit Recombinant Monoclonal DR4 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (AB312849), expandable thumbnail](https://content.abcam.com/products/images/dr4-antibody-epr28152-57-bsa-and-azide-free-ab312849--flow-cytometry-intracellular-img417288.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (AB312849)")
![Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (AB312849), expandable thumbnail](https://content.abcam.com/products/images/dr4-antibody-epr28152-57-bsa-and-azide-free-ab312849--immunocytochemistry-immunofluorescence-img417269.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (AB312849)")
![Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (AB312849), expandable thumbnail](https://content.abcam.com/products/images/dr4-antibody-epr28152-57-bsa-and-azide-free-ab312849--western-blot-img417195.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (AB312849)")
![Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (AB312849), expandable thumbnail](https://content.abcam.com/products/images/dr4-antibody-epr28152-57-bsa-and-azide-free-ab312849--western-blot-img417196.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (AB312849)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:26457518, PubMed:38532423). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PubMed:19090789). Promotes the activation of NF-kappa-B (PubMed:9430227).
Alternative names
CD261, APO2, DR4, TRAILR1, TNFRSF10A, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal DR4 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28152-57
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DR4 also known as TRAIL-R1 is a receptor with a molecular weight of approximately 60 kDa. It is part of the tumor necrosis factor receptor (TNFR) superfamily. DR4 is widely expressed in various types of tissues such as the liver spleen thymus and peripheral blood leukocytes. It functions mechanically by binding to its ligand TNF-related apoptosis-inducing ligand (TRAIL) which initiates signaling cascades that lead to apoptosis or programmed cell death.
- Biological function summary
The death receptor 4 plays an important role in mediating cell apoptosis. Upon ligand binding DR4 interacts with the adapter molecule FADD (FAS-associated protein with death domain) facilitating the formation of the death-inducing signaling complex (DISC). This complex recruits and activates caspase-8 which eventually leads to the activation of the downstream effector caspases driving the cell towards apoptosis. DR4 operates as a significant component of the extrinsic pathway of apoptosis a process important for maintaining cellular homeostasis and eliminating harmful cells.
- Pathways
DR4 functions critically within the extrinsic apoptotic pathway. This pathway involves other death receptors such as DR5 which also binds TRAIL similarly leading to apoptosis through DISC formation and caspase activation. The extrinsic pathway interconnects with the intrinsic pathway via the mitochondria with proteins like Bid acting as a bridge between them. The engagement of these pathways highlights DR4’s importance in regulatory mechanisms of cell death tightly controlling cellular proliferation and survival.
- Associated diseases and disorders
DR4 plays a significant role in cancer and autoimmune diseases. Alterations in DR4 expression or function can lead to evasion of apoptosis by cancer cells contributing to tumor progression. The defective apoptotic signaling via DR4 is also linked to autoimmune disorders where insufficient apoptosis may allow for the survival of autoreactive immune cells. In both scenarios DR4's involvement usually associates with its counterpart DR5 as they share similar ligand-binding properties and signal transduction mechanisms in diseased states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry (Intracellular) - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849), expandable thumbnail](https://content.abcam.com/products/images/dr4-antibody-epr28152-57-bsa-and-azide-free-ab312849--flow-cytometry-intracellular-img417288.jpg "Flow Cytometry (Intracellular) - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849)")
Flow Cytometry (Intracellular) - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849)
This data was developed using Anti-DR4 antibody [EPR28152-57] ab312848, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized TNFRSF10A (DR4) knockout HeLa line (Left) / Parental HeLa cell line (Right) cells labelling DR4 with Anti-DR4 antibody [EPR28152-57] ab312848 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849), expandable thumbnail](https://content.abcam.com/products/images/dr4-antibody-epr28152-57-bsa-and-azide-free-ab312849--immunocytochemistry-immunofluorescence-img417269.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849)")
Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849)
This data was developed using Anti-DR4 antibody [EPR28152-57] ab312848, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling DR4 with Anti-DR4 antibody [EPR28152-57] ab312848 at 1/50 (9.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in HCT 116 cell line. Negative control: 293T. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.![Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849), expandable thumbnail](https://content.abcam.com/products/images/dr4-antibody-epr28152-57-bsa-and-azide-free-ab312849--western-blot-img417195.jpg "Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849)")
Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849)
This data was developed using Anti-DR4 antibody [EPR28152-57] ab312848, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Performed under reducing conditions.
In Western blot, Anti-DR4 antibody [EPR28152-57] ab312848 was shown to bind specifically to TNFRSF10A (DR4). A band was observed at 45 kDa in wild-type Hela cell lysates whereas loss of signal was observed in the TNFRSF10A (DR4) knockout cell line Human TNFRSF10A (DR4) knockout HeLa cell line ab264687 (knockout cell lysate Human TNFRSF10A (DR4) knockout HeLa cell lysate ab258726).
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
Exposure time: 15 secondsAll lanes: Western blot - Anti-DR4 antibody [EPR28152-57] (Anti-DR4 antibody [EPR28152-57] ab312848) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 2: Western blot - Human TNFRSF10A (DR4) knockout HeLa cell lysate (Human TNFRSF10A (DR4) knockout HeLa cell lysate ab258726) at 50 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 45 kDa
Exposure time: 15s
![Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849), expandable thumbnail](https://content.abcam.com/products/images/dr4-antibody-epr28152-57-bsa-and-azide-free-ab312849--western-blot-img417196.jpg "Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849)")
Western blot - Anti-DR4 antibody [EPR28152-57] - BSA and Azide free (ab312849)
This data was developed using Anti-DR4 antibody [EPR28152-57] ab312848, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: 293T, Fadu.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 secondsAll lanes: Western blot - Anti-DR4 antibody [EPR28152-57] (Anti-DR4 antibody [EPR28152-57] ab312848) at 1/1000 dilution
Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 50 µg
Lane 2: Fadu (human pharyeal carcinoma epithelial cell) whole cell lysate at 50 µg
Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 45 kDa
Exposure time: 15s
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