Anti-DR5 antibody

• ICC

Supplier Data

Immunocytochemistry - Anti-DR5 antibody (AB8416)

ICC analysis of 4% paraformaldehyde-fixed (room temperature for 10 minutes) HeLa cells labeling DR5 with ab8416 at 20μg/mL, followed by goat anti-rabbit IgG antibody (Alexa Fluor 488) at 1/500 dilution (green).

Blocking condition : 5% BSA, room temperature for 1 hour.

Primary antibody incubation : 4°C overnight.

Secondary antibody incubation : room temperature for 1 hour.

Washing condition : PBS, 3 x 3 minutes

• ICC

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Immunocytochemistry - Anti-DR5 antibody (AB8416)

ab8416 at 5μg/ml staining DR5 in Hela cells by ICC. Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at room termperature. Antigen retrieval was by heat mediation with a Citrate buffer (pH 6). Overnight primary antibody at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (AB8416)

IHC-P of 4% paraformaldehyde-fixed rat brain tissue labelled DR5 with ab8416 at 5μg/ml, followed by Goat Anti-Rabbit IgG secondary antibody at 1/5000 dilution (green) and DAPI (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (AB8416)

Immunohistochemical analysis of 4% paraformaldehydefixed mouse kidney tissue using anti-DR5 antibody (ab8416) at 20μg/mL followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (AB8416)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DR5 antibody (ab8416) at 5μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation (microwave for 20 minutes) with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary at room temperature for 1 hour. Counter stained with Hematoxylin.

Endogenous peroxidase blocking : 3% H₂0₂ at room temperature for 10 minutes.

Washing : PBS, 2 x 5 minutes.

• WB

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Western blot - Anti-DR5 antibody (AB8416)

All lanes:

Western blot - Anti-DR5 antibody (ab8416) at 0.5 µg/mL

Lane 1:

HEK293 cell lysate at 15 µg

Lane 2:

A431 cell lysate at 15 µg

Lane 3:

A549 cell lysate at 15 µg

Lane 4:

Caco-2 cell lysate at 15 µg

Lane 5:

Daudi cell lysate at 15 µg

Lane 6:

HeLa cell lysate at 15 µg

Lane 7:

HepG2 cell lysate at 15 µg

Lane 8:

K562 cell lysate at 15 µg

Lane 9:

MCF7 cell lysate at 15 µg

Lane 10:

Jurkat cell lysate at 15 µg

Lane 11:

SK-N-SH cell lysate at 15 µg

Lane 12:

THP-1 cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 47 kDa

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• WB

Supplier Data

Western blot - Anti-DR5 antibody (AB8416)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DR5 antibody (ab8416) at 1 µg/mL

All lanes:

Rat Skeletal Muscle at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP Conjugate at 1/10000 dilution

Predicted band size: 47 kDa

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• WB

Supplier Data

Western blot - Anti-DR5 antibody (AB8416)

All lanes:

Western blot - Anti-DR5 antibody (ab8416) at 1 µg

Lane 1:

3T3/NIH cell lysate at 15 µg

Lane 2:

C2C12 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 47 kDa

false

• WB

CiteAb

Western blot - Anti-DR5 antibody (AB8416)

DR5 western blot using anti-DR5 antibody ab8416. Publication image and figure legend from Yang, Y., Zhang, P., et al., 2018, Front Pharmacol, PubMed 29946260.

ab8416 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab8416 please see the product overview.

Effect of SCG on protein expression of TRAIL, DR5, FASL, FAS, IL-33, and caspase-3 in Con A-treated mice. (A) The expression of TRAIL. (B) The expression of DR5. (C) The expression of IL-33. (D) The expression of FASL. (E) The expression of FAS. (F) The expression of cleaved caspase-3. Data were expressed as mean ± SD (n = 3). **p < 0.01 compared with normal group; #p < 0.05, ##p < 0.01 compared with model group.

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