Name: Anti-DR5 antibody [EPR19310]
SKU: ab199357
Rating: 5 (5 reviews)

Rabbit Recombinant Monoclonal DR5 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 16 publications.

Images

Western blot - Anti-DR5 antibody [EPR19310] (AB199357), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/70	Notes -

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Target data

See full target information TNFRSF10B

Function

Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.

Alternative names

CD262, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186, TNFRSF10B, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2

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Rabbit Recombinant Monoclonal DR5 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 16 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR19310
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DR5 also known as Death Receptor 5 TRAIL-R2 or TNFRSF10B is a protein critical in apoptosis. It is a transmembrane receptor with a mass of approximately 54 kDa. Researchers find it expressed mostly in tissues such as lung colon and prostate. DR5 functions by binding to its ligand TRAIL triggering the extrinsic apoptotic pathway. Through this mechanism DR5 initiates a cascade of caspase activation that leads to cell death.

Biological function summary

DR5 plays a significant role in the regulation of apoptosis particularly in cancer cells. It is a component of the TRAIL receptor complex which includes several other death receptors like DR4. This complex formation allows DR5 to mediate apoptotic signals more efficiently. By controlling apoptosis DR5 helps maintain tissue homeostasis and prevents abnormal cell proliferation.

Pathways

DR5 is an important part of the TRAIL signaling pathway and the extrinsic apoptosis pathway. It connects with proteins like FADD and Caspase-8 essential in the apoptotic signaling cascade. By interacting with these proteins DR5 drives the progression of the apoptotic signal ensuring the removal of dysfunctional cells. Such pathways are vital for restraining tumor development and progression.

Associated diseases and disorders

DR5 exhibits significant relevance to cancer and autoimmune diseases. Its ability to induce apoptosis through the TRAIL pathway keeps oncogenic transformations in check. In cancer DR5 often interfaces with other proteins like Bcl-2 which are involved in cell survival. Furthermore defects or aberrant expressions in DR5 have associations with autoimmune disorders where excessive cell death or survival contributes to disease pathology. DR5's role in these conditions makes it a promising target in therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Western blot - Anti-DR5 antibody [EPR19310] (ab199357)
Lanes 1- 2: Merged signal (red and green). Green - ab199357 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab199357 was shown to react with DR5 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human TNFRSF10B (DR5) knockout HeLa cell line ab264922 (knockout cell lysate Human TNFRSF10B (DR5) knockout HeLa cell lysate ab257748) was used. Wild-type HeLa and TNFRSF10B knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab199357 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-DR5 antibody [EPR19310] (ab199357) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: DR5 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DR5 antibody [EPR19310] (ab199357)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cell line) cells labeling DR5 with ab199357 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT1080 cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab199357 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
Western blot - Anti-DR5 antibody [EPR19310] (ab199357)
Lanes 1 - 2: Merged signal (red and green). Green - ab199357 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab199357 was shown to recognize DR5 in wild-type HAP1 cells as signal was lost at the expected MW in TNFRSF10B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TNFRSF10B knockout samples were subjected to SDS-PAGE. ab199357 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-DR5 antibody [EPR19310] (ab199357) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: TNFRSF10B knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 47 kDa, 72 kDa
Observed band size: 120 kDa, 72 kDa
Immunoprecipitation - Anti-DR5 antibody [EPR19310] (ab199357)
DR5 was immunoprecipitated from 0.35mg of HT1080 (Human fibrosarcoma cell line) treated with 5μM MG132 for 4 hour whole cell lysate with ab199357 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199357 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HT1080 treated with 5μM MG132 for 4 hour whole cell lysate, 10μg (Input).
Lane 2: ab199357 IP in HT1080 treated with 5μM MG132 for 4 hour whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199357 in HT1080 treated with 5μM MG132 for 4 hour whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-DR5 antibody [EPR19310] (ab199357)
Predicted band size: 47 kDa
Western blot - Anti-DR5 antibody [EPR19310] (ab199357)
Blocking/Dilution buffer: 5% NFDM/TBST.
Doxorubicin treatment elevated the expression of DR5 (PMID: 12496481; PMID: 11468181; PMID: 11090076). The expression profile is consistent with what has been described in the literature (PMID:20515924; PMID:16297203).
All lanes: Western blot - Anti-DR5 antibody [EPR19310] (ab199357) at 1/1000 dilution
Lane 1: Untreated HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 2: HCT 116 (Human colorectal carcinoma cell line) treated with 0.5μM/ml doxorubicin for 24 hours whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 40 kDa, 48 kDa
Exposure time: 8s
Immunoprecipitation - Anti-DR5 antibody [EPR19310] (ab199357)
DR5 was immunoprecipitated from 0.35mg of HCT 116 (Human colorectal carcinoma cell line) whole cell lysate with ab199357 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199357 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HCT 116 whole cell lysate, 10μg (Input).
Lane 2: ab199357 IP in HCT 116 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199357 in HCT 116 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-DR5 antibody [EPR19310] (ab199357)
Predicted band size: 47 kDa
Western blot - Anti-DR5 antibody [EPR19310] (ab199357)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-2: 3 minutes; Lane 3: 10 seconds; Lane 4: 8 seconds.
The expression profile is consistent with what has been described in the literature (PMID:20515924; PMID:16297203).
All lanes: Western blot - Anti-DR5 antibody [EPR19310] (ab199357) at 1/1000 dilution
Lane 1: Human melanoma lysate at 10 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
Lane 4: HT1080 (Human fibrosarcoma cell line) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 40 kDa, 48 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DR5 antibody [EPR19310] (ab199357)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (Human colorectal carcinoma cell line) cells labeling DR5 with ab199357 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining on HCT 116 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab199357 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-DR5 antibody [EPR19310] (ab199357)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HCT 116 (Human colorectal carcinoma cell line) cells labeling DR5with ab199357 at 1/70 dilution (red) compared with aRabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti Rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution was used as the secondary antibody.