Rabbit Recombinant Monoclonal DR5 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (AB251269), expandable thumbnail](https://content.abcam.com/products/images/dr5-antibody-epr19310-bsa-and-azide-free-ab251269--western-blot-img139577.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (AB251269)")
![Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (AB251269), expandable thumbnail](https://content.abcam.com/products/images/dr5-antibody-epr19310-bsa-and-azide-free-ab251269--western-blot-img150820.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (AB251269)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
1 products for Alternative Version
Target data
Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
Alternative names
Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, TRICK2, TNFRSF10B, DR5, KILLER, TRAILR2, UNQ160/PRO186, ZTNFR9
Recommended products
Rabbit Recombinant Monoclonal DR5 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, TRICK2, TNFRSF10B, DR5, KILLER, TRAILR2, UNQ160/PRO186, ZTNFR9
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19310
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab251269 is the carrier-free version of Anti-DR5 antibody [EPR19310] ab199357.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
DR5 also known as Death Receptor 5 TRAIL-R2 or TNFRSF10B is a protein critical in apoptosis. It is a transmembrane receptor with a mass of approximately 54 kDa. Researchers find it expressed mostly in tissues such as lung colon and prostate. DR5 functions by binding to its ligand TRAIL triggering the extrinsic apoptotic pathway. Through this mechanism DR5 initiates a cascade of caspase activation that leads to cell death.
- Biological function summary
DR5 plays a significant role in the regulation of apoptosis particularly in cancer cells. It is a component of the TRAIL receptor complex which includes several other death receptors like DR4. This complex formation allows DR5 to mediate apoptotic signals more efficiently. By controlling apoptosis DR5 helps maintain tissue homeostasis and prevents abnormal cell proliferation.
- Pathways
DR5 is an important part of the TRAIL signaling pathway and the extrinsic apoptosis pathway. It connects with proteins like FADD and Caspase-8 essential in the apoptotic signaling cascade. By interacting with these proteins DR5 drives the progression of the apoptotic signal ensuring the removal of dysfunctional cells. Such pathways are vital for restraining tumor development and progression.
- Associated diseases and disorders
DR5 exhibits significant relevance to cancer and autoimmune diseases. Its ability to induce apoptosis through the TRAIL pathway keeps oncogenic transformations in check. In cancer DR5 often interfaces with other proteins like Bcl-2 which are involved in cell survival. Furthermore defects or aberrant expressions in DR5 have associations with autoimmune disorders where excessive cell death or survival contributes to disease pathology. DR5's role in these conditions makes it a promising target in therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (ab251269), expandable thumbnail](https://content.abcam.com/products/images/dr5-antibody-epr19310-bsa-and-azide-free-ab251269--western-blot-img139577.jpg "Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (ab251269)")
Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (ab251269)
This data was developed using the same antibody clone in a different buffer formulation (Anti-DR5 antibody [EPR19310] ab199357).
Lanes 1- 2: Merged signal (red and green). Green - Anti-DR5 antibody [EPR19310] ab199357 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-DR5 antibody [EPR19310] ab199357 was shown to react with DR5 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human TNFRSF10B (DR5) knockout HeLa cell line ab264922 (knockout cell lysate Human TNFRSF10B (DR5) knockout HeLa cell lysate ab257748) was used. Wild-type HeLa and DR5 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-DR5 antibody [EPR19310] ab199357 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-DR5 antibody [EPR19310] (Anti-DR5 antibody [EPR19310] ab199357) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: DR5 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (ab251269), expandable thumbnail](https://content.abcam.com/products/images/dr5-antibody-epr19310-bsa-and-azide-free-ab251269--western-blot-img150820.jpg "Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (ab251269)")
Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (ab251269)
This data was developed using the same antibody clone in a different buffer formulation (Anti-DR5 antibody [EPR19310] ab199357).
Lanes 1 - 2: Merged signal (red and green). Green - Anti-DR5 antibody [EPR19310] ab199357 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-DR5 antibody [EPR19310] ab199357 was shown to recognize DR5 in wild-type HAP1 cells as signal was lost at the expected MW in TNFRSF10B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TNFRSF10B knockout samples were subjected to SDS-PAGE. Anti-DR5 antibody [EPR19310] ab199357 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-DR5 antibody [EPR19310] - BSA and Azide free (ab251269) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: TNFRSF10B knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 47 kDa
Observed band size: 47 kDa
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