JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB243847

Anti-DR5 antibody [EPR22276] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal DR5 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

View Alternative Names

CD262, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186, TNFRSF10B, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [EPR22276] - BSA and Azide free (AB243847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [EPR22276] - BSA and Azide free (AB243847)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DR5 with ab230969 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human colon cancer cells (PMID : 23284732) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230969).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [EPR22276] - BSA and Azide free (AB243847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [EPR22276] - BSA and Azide free (AB243847)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling DR5 with ab230969 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in endothelial cells of human endometrium (PMID : 22789540, 12874246, 15987601) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230969).

Immunoprecipitation - Anti-DR5 antibody [EPR22276] - BSA and Azide free (AB243847)
  • IP

Supplier Data

Immunoprecipitation - Anti-DR5 antibody [EPR22276] - BSA and Azide free (AB243847)

DR5 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate with ab230969 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230969 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1 : U-87 MG whole cell lysate 10 μg (Input).
Lane 2 : ab230969 IP in U-87 MG whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230969 in U-87 MG whole cell lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 20 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230969).

All lanes:

Immunoprecipitation - Anti-DR5 antibody [EPR22276] (<a href='/en-us/products/primary-antibodies/dr5-antibody-epr22276-ab230969'>ab230969</a>)

Predicted band size: 47 kDa

Observed band size: 40 kDa,48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22276

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab243847 is the carrier-free version of ab230969.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DR5 also known as Death Receptor 5 TRAIL-R2 or TNFRSF10B is a protein critical in apoptosis. It is a transmembrane receptor with a mass of approximately 54 kDa. Researchers find it expressed mostly in tissues such as lung colon and prostate. DR5 functions by binding to its ligand TRAIL triggering the extrinsic apoptotic pathway. Through this mechanism DR5 initiates a cascade of caspase activation that leads to cell death.
Biological function summary

DR5 plays a significant role in the regulation of apoptosis particularly in cancer cells. It is a component of the TRAIL receptor complex which includes several other death receptors like DR4. This complex formation allows DR5 to mediate apoptotic signals more efficiently. By controlling apoptosis DR5 helps maintain tissue homeostasis and prevents abnormal cell proliferation.

Pathways

DR5 is an important part of the TRAIL signaling pathway and the extrinsic apoptosis pathway. It connects with proteins like FADD and Caspase-8 essential in the apoptotic signaling cascade. By interacting with these proteins DR5 drives the progression of the apoptotic signal ensuring the removal of dysfunctional cells. Such pathways are vital for restraining tumor development and progression.

DR5 exhibits significant relevance to cancer and autoimmune diseases. Its ability to induce apoptosis through the TRAIL pathway keeps oncogenic transformations in check. In cancer DR5 often interfaces with other proteins like Bcl-2 which are involved in cell survival. Furthermore defects or aberrant expressions in DR5 have associations with autoimmune disorders where excessive cell death or survival contributes to disease pathology. DR5's role in these conditions makes it a promising target in therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed : 10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
See full target information TNFRSF10B
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB268264

Anti-DR5 antibody [DR5/3381] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [DR5/3381] - BSA and Azide free (AB268264)

  • 0
  • 0
Primary Antibodies

AB268140

Anti-DR5 antibody [DR5/3381]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [DR5/3381] (AB268140)

  • 0
  • 0
Primary Antibodies

AB8416

Anti-DR5 antibody

Immunocytochemistry - Anti-DR5 antibody (AB8416)

  • 4
  • 5
Primary Antibodies

AB166650

Anti-DR5 antibody [DR5-01-1]

Flow Cytometry - Anti-DR5 antibody [DR5-01-1] (AB166650)

  • 0
  • 0
Primary Antibodies

AB16329

Anti-DR5 antibody

Immunocytochemistry - Anti-DR5 antibody (AB16329)

  • 3
  • 1
Primary Antibodies

AB95619

PE Anti-DR5 antibody [MD5-1]

Flow Cytometry - PE Anti-DR5 antibody [MD5-1] (AB95619)

  • 0
  • 0
Primary Antibodies

AB53319

FITC Anti-DR5 antibody [DR5-01-1]

Flow Cytometry - FITC Anti-DR5 antibody [DR5-01-1] (AB53319)

  • 0
  • 0
Primary Antibodies

AB55863

PE Anti-DR5 antibody [DR5-01-1]

  • 0
  • 0
Primary Antibodies

AB199357

Anti-DR5 antibody [EPR19310]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-DR5 antibody [EPR19310] (AB199357)

  • 5
  • 5

Complementary Products

Assay Kits

AB46013

Human DR5 ELISA Kit (CD262)

Sandwich ELISA - Human DR5 ELISA Kit (CD262) (AB46013)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

  • 4
  • 28
Primary Antibodies

AB205921

Anti-PD-L1 antibody [28-8]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

  • 4
  • 15

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com