AB8419

Anti-DRAK2 antibody

Name: Anti-DRAK2 antibody (ab8419) | Abcam
Brand: Abcam Limited
SKU: AB8419
Rating: 2 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal DRAK2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human STK17B aa 350 to C-terminus.

View Alternative Names

DRAK2, STK17B, Serine/threonine-protein kinase 17B, DAP kinase-related apoptosis-inducing protein kinase 2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-DRAK2 antibody (AB8419)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DRAK2 antibody (AB8419)

ab8419 at 10μg/ml staining DRAK2 in Jurkat cells by ICC/IF

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Western blot - Anti-DRAK2 antibody (AB8419)

We are unsure about the nature of the 70 kDa band. However, DRAK2 is autophosphorylated and it is possible that this band corresponds to the phosphorylated form. The fact that the detection of this band is blocked by DRAK2 peptide indicates that it probably is closely related to the DRAK2 protein.

All lanes:

Western blot - Anti-DRAK2 antibody (ab8419) at 1/500 dilution

Lane 1:

Jurkat whole cell lysate with absence of blocking peptide

Lane 2:

Raji whole cell lysate with absence of blocking peptide

Lane 3:

Jurkat whole cell lysate with presence of blocking peptide

Lane 4:

Raji whole cell lysate with presence of blocking peptide

Predicted band size: 42 kDa

Observed band size: 45 kDa

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Western blot - Anti-DRAK2 antibody (AB8419)

Lane 1:

Western blot - Anti-DRAK2 antibody (ab8419) at 1 µg/mL

Lane 2:

Western blot - Anti-DRAK2 antibody (ab8419) at 2 µg/mL

All lanes:

Raji cell lysate

Predicted band size: 42 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human STK17B aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O94768

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

DRAK2 Antibody is Ion exchange chromatography purified.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DRAK2 also known as Death-associated protein kinase-related apoptosis-inducing protein kinase 2 is part of the serine/threonine kinase family. This protein has a molecular weight of about 48 kDa. DRAK2 expresses mostly in lymphoid tissues including the spleen and thymus. It plays a role in negative regulation during T-cell receptor signaling and is important during lymphocyte activation.

Biological function summary

The role of DRAK2 extends beyond kinase activity. It is involved in immune response modulation and can affect apoptosis in T lymphocytes. DRAK2 is not part of a larger protein complex but rather acts independently to exert its function. It influences cellular mechanisms by altering signaling pathways that impact cell survival and death.

Pathways

You find DRAK2 involved in both the T-cell receptor signaling and apoptosis pathways. It connects to the T-cell receptor pathway due to its regulation of T-cell activation and proliferation. DRAK2 activity can interact with proteins such as calmodulin and Protein Kinase C in these pathways indicating its role in signaling transduction critical for T-cell function.

DRAK2 links to autoimmune diseases and cancer. Abnormal DRAK2 expression might contribute to autoimmune conditions like multiple sclerosis by affecting T-cell autoreactivity. Its involvement in cancer emerges through regulation of apoptosis where malfunction may lead to resistance against cell death aiding cancer cell survival. In this context DRAK2 connection with proteins such as caspases plays a role in mediating apoptotic pathways in cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis.

See full target information STK17B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:4558 PubMed38402348

2024

Biomarker screening using integrated bioinformatics for the development of "normal-impaired glucose intolerance-type 2 diabetes mellitus".

Applications

Unspecified application

Species

Unspecified reactive species

Dongqiang Luo,Xiaolu Gao,Xianqiong Zhu,Jiongbo Xu,Pengfei Gao,Jiayi Zou,Qiaoming Fan,Ying Xu,Tian Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DRAK2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DRAK2 antibody (AB8419)

Western blot - Anti-DRAK2 antibody (AB8419)

Western blot - Anti-DRAK2 antibody (AB8419)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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