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AB108373

Anti-DRAK2 antibody [EPR3163Y]

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(1 Publication)

Rabbit Recombinant Monoclonal DRAK2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

DRAK2, STK17B, Serine/threonine-protein kinase 17B, DAP kinase-related apoptosis-inducing protein kinase 2

6 Images
Western blot - Anti-DRAK2 antibody [EPR3163Y] (AB108373)
  • WB

Lab

Western blot - Anti-DRAK2 antibody [EPR3163Y] (AB108373)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/1000 dilution

Lane 1:

C6 whole cell lysate at 10 µg

Lane 2:

PC-12 whole cell lysate at 10 µg

Lane 3:

NIH/3T3 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 42 kDa,59 kDa

Observed band size: 42 kDa

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Western blot - Anti-DRAK2 antibody [EPR3163Y] (AB108373)
  • WB

Lab

Western blot - Anti-DRAK2 antibody [EPR3163Y] (AB108373)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : DRAK2 knockout HAP1 cell lysate (20 μg)
Lane 3 : Ramos cell lysate (20 μg)
Lane 4 : Raji cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab108373 observed at 42 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108373 was shown to recognize DRAK2 when DRAK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and DRAK2 knockout samples were subjected to SDS-PAGE. ab108373 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-DRAK2 antibody [EPR3163Y] (ab108373)

Predicted band size: 42 kDa

false

Western blot - Anti-DRAK2 antibody [EPR3163Y] (AB108373)
  • WB

Lab

Western blot - Anti-DRAK2 antibody [EPR3163Y] (AB108373)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/10000 dilution

Lane 1:

Ramos whole cell lysate at 10 µg

Lane 2:

Raji whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-DRAK2 antibody [EPR3163Y] (AB108373)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DRAK2 antibody [EPR3163Y] (AB108373)

Immunofluorescence staining of Ramos cells with purified ab108373 at a working dilution of 1/100 counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077) used at a dilution of 1/1000. ab7291 a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse 1/1000) shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1 purified ab108373 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2 ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

Immunoprecipitation - Anti-DRAK2 antibody [EPR3163Y] (AB108373)
  • IP

Unknown

Immunoprecipitation - Anti-DRAK2 antibody [EPR3163Y] (AB108373)

ab108373 (purified) at 1/40 immunoprecipitating DRAK2 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 42 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-DRAK2 antibody [EPR3163Y] (ab108373)

Predicted band size: 39 kDa,42 kDa

false

Western blot - Anti-DRAK2 antibody [EPR3163Y] (AB108373)
  • WB

Unknown

Western blot - Anti-DRAK2 antibody [EPR3163Y] (AB108373)

All lanes:

Western blot - Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/500 dilution

Lane 1:

Jurkat cell lysate +TPA at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

Ramos cell lysate + TPA at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

  • Carrier free

    Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3163Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DRAK2 also known as Death-associated protein kinase-related apoptosis-inducing protein kinase 2 is part of the serine/threonine kinase family. This protein has a molecular weight of about 48 kDa. DRAK2 expresses mostly in lymphoid tissues including the spleen and thymus. It plays a role in negative regulation during T-cell receptor signaling and is important during lymphocyte activation.
Biological function summary

The role of DRAK2 extends beyond kinase activity. It is involved in immune response modulation and can affect apoptosis in T lymphocytes. DRAK2 is not part of a larger protein complex but rather acts independently to exert its function. It influences cellular mechanisms by altering signaling pathways that impact cell survival and death.

Pathways

You find DRAK2 involved in both the T-cell receptor signaling and apoptosis pathways. It connects to the T-cell receptor pathway due to its regulation of T-cell activation and proliferation. DRAK2 activity can interact with proteins such as calmodulin and Protein Kinase C in these pathways indicating its role in signaling transduction critical for T-cell function.

DRAK2 links to autoimmune diseases and cancer. Abnormal DRAK2 expression might contribute to autoimmune conditions like multiple sclerosis by affecting T-cell autoreactivity. Its involvement in cancer emerges through regulation of apoptosis where malfunction may lead to resistance against cell death aiding cancer cell survival. In this context DRAK2 connection with proteins such as caspases plays a role in mediating apoptotic pathways in cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis.
See full target information STK17B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The European journal of neuroscience 54:6673-6684 PubMed34536317

2021

Serine/threonine kinase 17b (STK17B) signalling regulates Purkinje cell dendritic development and is altered in multiple spinocerebellar ataxias.

Applications

WB

Species

Mouse

Qin-Wei Wu,Josef P Kapfhammer
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB8419

Anti-DRAK2 antibody

primary-antibodies

drak2-antibody-ab8419

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(1 reviews)

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