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AB64739

Anti-DRAM antibody [M3-P4B4]

4

(2 Reviews)

|

(1 Publication)

Mouse Monoclonal DRAM antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DRAM, DRAM1, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator

1 Images
Western blot - Anti-DRAM antibody [M3-P4B4] (AB64739)
  • WB

Project6996****

Western blot - Anti-DRAM antibody [M3-P4B4] (AB64739)

All lanes:

Western blot - Anti-DRAM antibody [M3-P4B4] (ab64739) at 10 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Exposure time: 20min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M3-P4B4

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DRAM (Damage-Regulated Autophagy Modulator) protein also known by its alternate name "drum" plays a significant role in the regulation of autophagy. DRAM serves as an effector molecule that interacts with specific molecular pathways to control cell self-digestion processes. Its molecular weight falls within the range typical for small regulatory proteins. Expression of DRAM mainly occurs in various tissues with a higher presence observed in human epithelial tissues.
Biological function summary

DRAM facilitates the autophagy process which is critical for cellular homeostasis and balancing the degradation and recycling of cellular components. It functions closely with other autophagy-related proteins forming part of a complex that includes well-studied regulators like ULK1 and LAMP2. The modulation of autophagic activity by DRAM has implications for both normal cellular processes and the response to cellular stresses.

Pathways

The product of the DRAM gene integrates into the autophagic mechanisms where it acts to enhance degradation of defective proteins and organelles. It has associations within the p53 signaling pathway through which it mediates apoptosis and autophagy. Proteins such as p53 and BCL-2 are closely related to the action of DRAM influencing cellular fate decisions during stress conditions.

DRAM's role in autophagy links it to cancer and neurodegenerative diseases. Upregulated DRAM expression is frequently observed in tumor cells where it may influence cancer progression by modulating cell survival pathways. Additionally dysregulated autophagy involving DRAM has connections to Alzheimer's disease potentially due to interactions with proteins like APP which undergo autophagic degradation processes that this protein modulates.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.
See full target information DRAM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36552800

2022

FUT2 Facilitates Autophagy and Suppresses Apoptosis via p53 and JNK Signaling in Lung Adenocarcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Zhang,Enze Yao,Yijing Liu,Yining Zhang,Mengyang Ding,Jingyu Liu,Xiaoming Chen,Sairong Fan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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