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AB325852

Anti-DRD4 antibody [HL2851] - BSA and Azide free

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Rabbit Recombinant Monoclonal DRD4 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Immunogen corresponding to Synthetic Peptide within Human DRD4.

View Alternative Names

D(4) dopamine receptor, D(2C) dopamine receptor, Dopamine D4 receptor, DRD4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRD4 antibody [HL2851] - BSA and Azide free (AB325852)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRD4 antibody [HL2851] - BSA and Azide free (AB325852)

ab325852 detects Dopamine Receptor D4 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded human stomach tumor. Dopamine Receptor D4 stained by ab325852 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-DRD4 antibody [HL2851] - BSA and Azide free (AB325852)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DRD4 antibody [HL2851] - BSA and Azide free (AB325852)

ab325852 detects Dopamine Receptor D4 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : Dopamine Receptor D4 stained by ab325852 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-DRD4 antibody [HL2851] - BSA and Azide free (AB325852)
  • WB

Supplier Data

Western blot - Anti-DRD4 antibody [HL2851] - BSA and Azide free (AB325852)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325852 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-DRD4 antibody [HL2851] - BSA and Azide free (ab325852) at 1/5000 dilution

Lanes 1, 3, 5, 7 and 9:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled DRD1 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled DRD2 transfected (+) 293T whole cell extracts at 30 µg

Lane 6:

Unboiled DRD3 transfected (+) 293T whole cell extracts at 30 µg

Lane 8:

Unboiled DRD4 transfected (+) 293T whole cell extracts at 30 µg

Lane 10:

Unboiled DRD5 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-DRD4 antibody [HL2851] - BSA and Azide free (AB325852)
  • WB

Supplier Data

Western blot - Anti-DRD4 antibody [HL2851] - BSA and Azide free (AB325852)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325852 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-DRD4 antibody [HL2851] - BSA and Azide free (ab325852) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged DRD4 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged DRD4 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2851

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human DRD4.

P21917

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Activated by dopamine, but also by epinephrine and norepinephrine, and by numerous synthetic agonists and drugs (PubMed : 16423344, PubMed : 27659709, PubMed : 29051383, PubMed : 9003072). Agonist binding triggers signaling via G proteins that inhibit adenylyl cyclase (PubMed : 16423344, PubMed : 27659709, PubMed : 29051383, PubMed : 7512953, PubMed : 7643093). Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells (By similarity).
See full target information DRD4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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