JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325853

Anti-DRD5 antibody [HL3081] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal DRD5 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Transfected cell lysate - Human, Human, Mouse, Transfected cell line - Human samples. Immunogen corresponding to Synthetic Peptide within Human DRD5.

View Alternative Names

DRD1B, DRD1L2, DRD5, D(1B) dopamine receptor, D(5) dopamine receptor, D1beta dopamine receptor, Dopamine D5 receptor

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)

ab325853 detects Dopamine Receptor D5 protein by immunofluorescent analysis. Sample : SH-SY-5Y cells were fixed in ice-cold MeOH for 5 min. Green : Dopamine Receptor D5 stained by ab325853 diluted at 1 : 500. Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)

ab325853 detects Dopamine Receptor D5 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. Dopamine Receptor D5 stained by ab325853 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)

ab325853 detects Dopamine Receptor D5 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. Dopamine Receptor D5 stained by ab325853 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)

ab325853 detects Dopamine Receptor D5 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : Dopamine Receptor D5 stained by ab325853 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)
  • WB

Supplier Data

Western blot - Anti-DRD5 antibody [HL3081] - BSA and Azide free (AB325853)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325853 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-DRD5 antibody [HL3081] - BSA and Azide free (ab325853) at 1/5000 dilution

Lanes 1, 3, 5, 7 and 9:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled DRD1 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled DRD2 transfected (+) 293T whole cell extracts at 30 µg

Lane 6:

Unboiled DRD3 transfected (+) 293T whole cell extracts at 30 µg

Lane 8:

Unboiled DRD4 transfected (+) 293T whole cell extracts at 30 µg

Lane 10:

Unboiled DRD5 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL3081

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human DRD5.

P21918

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
See full target information DRD5
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com