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AB61770

Anti-DREAM antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal DREAM antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KCNIP3.

View Alternative Names

CSEN, DREAM, KCHIP3, KCNIP3, Calsenilin, A-type potassium channel modulatory protein 3, DRE-antagonist modulator, Kv channel-interacting protein 3, KChIP3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (AB61770)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (AB61770)

ICC/IF image of ab61770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61770, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DREAM antibody (AB61770)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DREAM antibody (AB61770)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61770 at a dilution of 1/50. left : untreated sample. Right : sample treated with immunising peptide.

Western blot - Anti-DREAM antibody (AB61770)
  • WB

Unknown

Western blot - Anti-DREAM antibody (AB61770)

All lanes:

Western blot - Anti-DREAM antibody (ab61770) at 1/500 dilution

Lane 1:

extracts from A431 cells with no peptide

Lane 2:

extracts from A431 cells with peptide

Predicted band size: 29 kDa

Observed band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human KCNIP3. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DREAM also called Downstream Regulatory Element Antagonist Modulator is a calcium-binding protein with a molecular mass of about 29 kDa. It is expressed in various tissues but shows higher levels in the brain and heart. DREAM belongs to the neuronal calcium sensory (NCS) family and acts as a transcriptional repressor that binds to the downstream regulatory element (DRE) sites on DNA. Through its calcium-binding ability DREAM modulates gene expression by changing its conformation depending on intracellular calcium levels.
Biological function summary

Calcium-binding proteins influence multiple cellular processes and DREAM participates in calcium signaling pathways important for neuronal function and gene transcription regulation. As part of the complex interactions within the cell DREAM modulates the expression of target genes by interacting with DRE sequences. Its regulatory role extends to processes including synaptic plasticity neuronal excitability and long-term depression. DREAM's function as a modulator of transcription links it to a broader regulatory network within cells.

Pathways

DREAM engages in calcium signaling pathways and is significant in the transcriptional regulation of genes involved in neuronal processes. DREAM interacts with CREB (cAMP response element-binding protein) and influences downstream gene expression in response to calcium fluctuations impacting the CREB signaling pathway. Additionally DREAM participates in pathways connected to the regulation of pain and inflammatory responses linking to proteins such as NF-kB which plays a role in immune response regulation.

Aberrations in DREAM function associate with neurodegenerative diseases and chronic pain conditions. Its regulatory role in neuronal gene expression connects DREAM to disorders like Alzheimer's and neuropathic pain. In these contexts altered DREAM activity can impact the expression of NF-kB and CREB target genes which are critical in mediating inflammatory responses and neuronal survival. Understanding DREAM's interactions and regulation could therefore provide insights into potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity).. Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of amyloid-beta formation.
See full target information KCNIP3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:884031 PubMed35711359

2022

Mechanical Strain Induces Transcriptomic Reprogramming of Saphenous Vein Progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Davide Maselli,Gloria Garoffolo,Giada Andrea Cassanmagnago,Rosa Vono,Matthijs S Ruiter,Anita C Thomas,Paolo Madeddu,Maurizio Pesce,Gaia Spinetti

Life sciences 227:166-174 PubMed31026452

2019

Vasopressin regulates hypothalamic GnRH synthesis: Histomorphological evidence in hypothalamus and biological effects in GT1-7 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Zhu,Xiaozhen Zhao,Feng Huang,Feng Wang,Wei Wang
View all publications
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Product promise

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