AB326351

Anti-DREAM antibody [X12]

Recombinant
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Rabbit Recombinant Monoclonal DREAM antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Rat, Mouse samples.

View Alternative Names

Csen, Dream, Kchip3, Kcnip3, Calsenilin, A-type potassium channel modulatory protein 3, DRE-antagonist modulator, Kv channel-interacting protein 3, DREAM, KChIP3, Csen, Dream, Kchip3, Calsenilin, A-type potassium channel modulatory protein 3, DRE-antagonist modulator, Kv channel-interacting protein 3, DREAM, KChIP3

3 Images

Supplier Data

Western blot - Anti-DREAM antibody [X12] (AB326351)

Western blot analysis showing detection of Mouse DREAM protein in a Mouse Brain lysate. Block : 5% BSA for 1 hour at RT. Primary Antibody : ab326351 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 40 seconds.

All lanes:

Western blot - Anti-DREAM antibody [X12] (ab326351) at 1/1000 dilution

All lanes:

Mouse Brain lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 40s

Supplier Data

Western blot - Anti-DREAM antibody [X12] (AB326351)

Western blot analysis showing detection of Rat DREAM protein in a Rat brain membrane lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326351 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 2 minutes 55 seconds.

All lanes:

Western blot - Anti-DREAM antibody [X12] (ab326351) at 1/1000 dilution

All lanes:

Rat brain membrane lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 175s

Supplier Data

Western blot - Anti-DREAM antibody [X12] (AB326351)

Western blot analysis showing detection of Human DREAM protein in a transient over-expression lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326351 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 1 min at RT in the dark. Exposed 6.584 seconds. NOTE : The ORF clone contains a C-term DDK tag; results were confirmed using Anti-DDK antibody, showing banding above target is related to oligomerization of the DREAM protein (data not shown).

All lanes:

Western blot - Anti-DREAM antibody [X12] (ab326351) at 1/1000 dilution

Lane 1:

HEK293T OE Lysate at 2.5 µg

Lane 2:

HEK293T Neg. Ctrl at 2.5 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 6.584s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

X12

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity).. Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (By similarity).. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of amyloid-beta formation (By similarity).

See full target information Kcnip3

Additional targets

Calsenilin

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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