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AB11068

Anti-Drebrin antibody

5

(5 Reviews)

|

(27 Publications)

Rabbit Polyclonal Drebrin antibody. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Chicken, Human samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human DBN1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

D0S117E, DBN1, Drebrin, Developmentally-regulated brain protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (AB11068)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (AB11068)

ICC/IF image of ab11068 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody (AB11068)
  • IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody (AB11068)

Rabbit polyclonal to Drebrin (ab11068) used in IHC(Formalin/PFA-fixed paraffin-embedded sections; Standard stABCpx-DAB detection). ab11068 detecting Drebrin protein on Rat spinal cord E16 embryo sections fixed in formaldehyde. Antigen retrieval step : Heat mediated. 1% BSA as blocking agent for 10 mins at RT. ab11068 was used at a dilution of 1/2500 incubated for 16 hours. Secondary Antibody is biotin labeled anti rabbit IgG (1/300). Drebrin positivity conformed to published localisation : essentially, many non-neuronal developing tissues were positive, eg : skeletal primary myotubes. This picture is of spinal cord floor plate region (x20). Nuclei were counterstained using Mayer's Haemalum.

This image is courtesy of Carl Hobbs, King's College London, United Kingdom

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (AB11068)
  • ICC/IF

AbReview14977****

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (AB11068)

ab11068 staining Drebrin in mouse COR-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton x100 and blocking with 2% BSA was done at 250C for 15 minutes. Samples were incubated with primary antibody (1/100 : in 1% BSA, 0.25% gelatin in PBS) for 3 hours at 25°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

This image is a courtesy of Anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse, Chicken

Applications

ICC, ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human DBN1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q16643

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations.</p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations.</p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections (PubMed : 20215400). Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS (PubMed : 20215400). Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity).
See full target information DBN1
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 43:100921 PubMed37603953

2023

Therapeutic HDAC inhibition in hypermutant diffuse intrinsic pontine glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa Noll,Carrie Myers,Matthew C Biery,Michael Meechan,Sophie Tahiri,Asmitha Rajendran,Michael E Berens,Danyelle Paine,Sara Byron,Jiaming Zhang,Conrad Winter,Fiona Pakiam,Sarah E S Leary,Bonnie L Cole,Evangeline R Jackson,Matthew D Dun,Jessica B Foster,Myron K Evans,Siobhan S Pattwell,James M Olson,Nicholas A Vitanza

Cell reports 40:111188 PubMed35977504

2022

Neuronal paxillin and drebrin mediate BDNF-induced force transduction and growth cone turning in a soft-tissue-like environment.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Chen,Chien-Hsin Chu,Ying Chu,Ting-Ya Chang,Sheng-Wen Chen,Shu-Yang Liang,Yun-Chi Tsai,Bi-Chang Chen,Hsiung-Lin Tu,Pei-Lin Cheng

Anesthesiology 137:212-231 PubMed35504002

2022

Isoflurane Disrupts Postsynaptic Density-95 Protein Interactions Causing Neuronal Synapse Loss and Cognitive Impairment in Juvenile Mice via Canonical NO-mediated Protein Kinase-G Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Swati Agarwal,Michele L Schaefer,Caroline Krall,Roger A Johns

Cells 10: PubMed34685539

2021

Nano-Infrared Imaging of Primary Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Raul O Freitas,Adrian Cernescu,Anders Engdahl,Agnes Paulus,João E Levandoski,Isak Martinsson,Elke Hebisch,Christophe Sandt,Gunnar Keppler Gouras,Christelle N Prinz,Tomas Deierborg,Ferenc Borondics,Oxana Klementieva

Frontiers in cell and developmental biology 9:634347 PubMed33777942

2021

Cerebral Ischemia-Reperfusion Is Associated With Upregulation of Cofilin-1 in the Motor Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Shu Xu,Lei-Miao Yin,Ai-Fang Cheng,Ying-Jie Zhang,Di Zhang,Miao-Miao Tao,Yun-Yi Deng,Lin-Bao Ge,Chun-Lei Shan

Acta neuropathologica communications 9:22 PubMed33546775

2021

Non-cell autonomous astrocyte-mediated neuronal toxicity in prion diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Rajesh Kushwaha,Anshuman Sinha,Natallia Makarava,Kara Molesworth,Ilia V Baskakov

The EMBO journal 39:e104870 PubMed32896909

2020

HIV-1 capsids mimic a microtubule regulator to coordinate early stages of infection.

Applications

Unspecified application

Species

Unspecified reactive species

Eveline Santos da Silva,Shanmugapriya Shanmugapriya,Viacheslav Malikov,Feng Gu,M Keegan Delaney,Mojgan H Naghavi

Nutrients 12: PubMed31963684

2020

The Preparation of Ginsenoside Rg5, Its Antitumor Activity against Breast Cancer Cells and Its Targeting of PI3K.

Applications

Unspecified application

Species

Unspecified reactive species

Yannan Liu,Daidi Fan

Molecular and cellular neurosciences 95:86-95 PubMed30763689

2019

APP depletion alters selective pre- and post-synaptic proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Isak Martinsson,Estibaliz Capetillo-Zarate,Mathilde Faideau,Katarina Willén,Noemi Esteras,Susanne Frykman,Lars O Tjernberg,Gunnar K Gouras

Current biology : CB 28:2081-2093.e6 PubMed29910073

2018

Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Schätzle,Marta Esteves da Silva,Roderick P Tas,Eugene A Katrukha,Hai Yin Hu,Corette J Wierenga,Lukas C Kapitein,Casper C Hoogenraad
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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