Rabbit Polyclonal Drebrin antibody. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Chicken, Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human DBN1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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- Cell reports 40:1111882022Neuronal paxillin and drebrin mediate BDNF-induced force transduction and growth cone turning in a soft-tissue-like environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Chen,Chien-Hsin Chu,Ying Chu,Ting-Ya Chang,Sheng-Wen Chen,Shu-Yang Liang,Yun-Chi Tsai,Bi-Chang Chen,Hsiung-Lin Tu,Pei-Lin ChengPubMed 35977504
- Anesthesiology 137:212-2312022Isoflurane Disrupts Postsynaptic Density-95 Protein Interactions Causing Neuronal Synapse Loss and Cognitive Impairment in Juvenile Mice via Canonical NO-mediated Protein Kinase-G Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSwati Agarwal,Michele L Schaefer,Caroline Krall,Roger A JohnsPubMed 35504002
- Cells 10:2021Nano-Infrared Imaging of Primary Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaul O Freitas,Adrian Cernescu,Anders Engdahl,Agnes Paulus,João E Levandoski,Isak Martinsson,Elke Hebisch,Christophe Sandt,Gunnar Keppler Gouras,Christelle N Prinz,Tomas Deierborg,Ferenc Borondics,Oxana KlementievaPubMed 34685539
- Frontiers in cell and developmental biology 9:6343472021Cerebral Ischemia-Reperfusion Is Associated With Upregulation of Cofilin-1 in the Motor Cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Shu Xu,Lei-Miao Yin,Ai-Fang Cheng,Ying-Jie Zhang,Di Zhang,Miao-Miao Tao,Yun-Yi Deng,Lin-Bao Ge,Chun-Lei ShanPubMed 33777942
- Acta neuropathologica communications 9:222021Non-cell autonomous astrocyte-mediated neuronal toxicity in prion diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajesh Kushwaha,Anshuman Sinha,Natallia Makarava,Kara Molesworth,Ilia V BaskakovPubMed 33546775
- The EMBO journal 39:e1048702020HIV-1 capsids mimic a microtubule regulator to coordinate early stages of infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEveline Santos da Silva,Shanmugapriya Shanmugapriya,Viacheslav Malikov,Feng Gu,M Keegan Delaney,Mojgan H NaghaviPubMed 32896909
- Nutrients 12:2020The Preparation of Ginsenoside Rg5, Its Antitumor Activity against Breast Cancer Cells and Its Targeting of PI3K.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYannan Liu et. al.PubMed 31963684
- Molecular and cellular neurosciences 95:86-952019APP depletion alters selective pre- and post-synaptic proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsak Martinsson et. al.PubMed 30763689
- Current biology : CB 28:2081-2093.e62018Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhilipp Schätzle et. al.PubMed 29910073
- eLife 6:2017Inter-dependent apical microtubule and actin dynamics orchestrate centrosome retention and neuronal delamination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIoannis Kasioulis et. al.PubMed 29058679
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DBN1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q16643
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Reactivity data
Select an application| ICC | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested |
| Mouse | Expected | Expected | Predicted | Predicted |
| Rat | Expected | Expected | Tested | Expected |
| Chicken | Expected | Expected | Predicted | Predicted |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. |
Species Rat | Dilution info - | Notes ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. |
Species Chicken | Dilution info - | Notes ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. |
Species Human | Dilution info - | Notes ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info - | Notes - |
Associated Products
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Target data
Function
Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections (PubMed:20215400). Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS (PubMed:20215400). Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity).
Alternative names
D0S117E, DBN1, Drebrin, Developmentally-regulated brain protein
Recommended products
Rabbit Polyclonal Drebrin antibody. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Chicken, Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human DBN1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DBN1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q16643
- Purity
- IgG fraction
- Concentration
- Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody (ab11068)
Rabbit polyclonal to Drebrin (ab11068) used in IHC(Formalin/PFA-fixed paraffin-embedded sections; Standard stABCpx-DAB detection). ab11068 detecting Drebrin protein on Rat spinal cord E16 embryo sections fixed in formaldehyde. Antigen retrieval step: Heat mediated. 1% BSA as blocking agent for 10 mins at RT. ab11068 was used at a dilution of 1/2500 incubated for 16 hours. Secondary Antibody is biotin labeled anti rabbit IgG (1/300). Drebrin positivity conformed to published localisation: essentially, many non-neuronal developing tissues were positive, eg: skeletal primary myotubes. This picture is of spinal cord floor plate region (x20). Nuclei were counterstained using Mayer's Haemalum.
This image is a courtesy of Anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab11068)
ab11068 staining Drebrin in mouse COR-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton x100 and blocking with 2% BSA was done at 250C for 15 minutes. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.25% gelatin in PBS) for 3 hours at 25°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab11068)
ICC/IF image of ab11068 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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