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AB178408

Anti-Drebrin antibody [EPR12634]

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(5 Publications)

Rabbit Recombinant Monoclonal Drebrin antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

D0S117E, DBN1, Drebrin, Developmentally-regulated brain protein

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408)

Immunofluorescent analysis of SH-SY5Y cells labeling Drebrin with ab178408 at 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408)

ab178408 staining Drebrin in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control : PBS only.

Western blot - Anti-Drebrin antibody [EPR12634] (AB178408)
  • WB

Supplier Data

Western blot - Anti-Drebrin antibody [EPR12634] (AB178408)

All lanes:

Western blot - Anti-Drebrin antibody [EPR12634] (ab178408) at 1/1000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

PC-12 lysate at 10 µg

Lane 3:

Neuro-2a lysate at 10 µg

Lane 4:

Fetal brain tissue lysate at 10 µg

Lane 5:

SH-SY5Y cell lysate at 10 µg

Lane 6:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 71 kDa

Observed band size: 120 kDa

false

Western blot - Anti-Drebrin antibody [EPR12634] (AB178408)
  • WB

Lab

Western blot - Anti-Drebrin antibody [EPR12634] (AB178408)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : Drebrin knockout HAP1 whole cell lysate (20 μg)
Lane 3 : SH-SY5Y whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab178408 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab178408 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. ab178408 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)

Predicted band size: 71 kDa

false

Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (AB178408)
  • IP

Supplier Data

Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (AB178408)

Immunoprecipitation. ab178408 at 1/1000 labeling Debrin in fetal brain lysate immunoprecipitated using ab178408 at 1/10.

All lanes:

Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (ab178408)

Predicted band size: 71 kDa

Observed band size: 120 kDa

false

  • Carrier free

    Anti-Drebrin antibody [EPR12634] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12634

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections (PubMed : 20215400). Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS (PubMed : 20215400). Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity).
See full target information DBN1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell insight 3:100183 PubMed39148723

2024

ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Luo,Jiannan Wu,Xueman Chen,Yulan Liu,Dequan Liu,Erwei Song,Man-Li Luo

Journal of extracellular vesicles 13:e12439 PubMed38647111

2024

Neuropathogenic role of astrocyte-derived extracellular vesicles in HIV-associated neurocognitive disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Divya T Chemparathy,Sudipta Ray,Chase Ochs,Natasha Ferguson,Dinesh Y Gawande,Shashank M Dravid,Shannon Callen,Susmita Sil,Shilpa Buch

Journal of inflammation (London, England) 20:15 PubMed37127610

2023

First trimester human umbilical cord perivascular cells (HUCPVC) modulate the kynurenine pathway and glutamate neurotransmission in an LPS-induced mouse model of neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Fyyaz Siddiqui,Denis Gallagher,Hannah Shuster-Hyman,Lianet Lopez,Andrée Gauthier-Fisher,Clifford L Librach

Scientific reports 13:6220 PubMed37069238

2023

Effects of 660-nm LED photobiomodulation on drebrin expression pattern and astrocyte migration.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Ryeong Yoon,So-Young Chang,Min Young Lee,Jin-Chul Ahn

Cells 11: PubMed35563735

2022

Astrocytes Derived from Familial and Sporadic Alzheimer's Disease iPSCs Show Altered Calcium Signaling and Respond Differently to Misfolded Protein Tau.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Brezovakova,Eva Sykova,Santosh Jadhav
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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