Rabbit Recombinant Monoclonal Drebrin antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Images
![Western blot - Anti-Drebrin antibody [EPR12634] (AB178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--western-blot-img125515.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Drebrin antibody [EPR12634] (AB178408)")
![Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--immunocytochemistry-immunofluorescence-img18048.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408)")
![Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (AB178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--immunoprecipitation-img77249.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (AB178408)")
![Western blot - Anti-Drebrin antibody [EPR12634] (AB178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--western-blot-img103223.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Drebrin antibody [EPR12634] (AB178408)")
![Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--immunocytochemistry-immunofluorescence-img90392.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408)")
Publications
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- Journal of inflammation (London, England) 20:152023First trimester human umbilical cord perivascular cells (HUCPVC) modulate the kynurenine pathway and glutamate neurotransmission in an LPS-induced mouse model of neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFyyaz Siddiqui,Denis Gallagher,Hannah Shuster-Hyman,Lianet Lopez,Andrée Gauthier-Fisher,Clifford L LibrachPubMed 37127610
- Scientific reports 13:62202023Effects of 660-nm LED photobiomodulation on drebrin expression pattern and astrocyte migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung Ryeong Yoon,So-Young Chang,Min Young Lee,Jin-Chul AhnPubMed 37069238
- Cells 11:2022Astrocytes Derived from Familial and Sporadic Alzheimer's Disease iPSCs Show Altered Calcium Signaling and Respond Differently to Misfolded Protein Tau.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronika Brezovakova,Eva Sykova,Santosh JadhavPubMed 35563735
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested |
| Mouse | Expected | Not recommended | Tested | Not recommended | Expected |
| Rat | Expected | Not recommended | Tested | Not recommended | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections (PubMed:20215400). Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS (PubMed:20215400). Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity).
Alternative names
D0S117E, DBN1, Drebrin, Developmentally-regulated brain protein
Recommended products
Rabbit Recombinant Monoclonal Drebrin antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12634
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Drebrin antibody [EPR12634] (ab178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--western-blot-img125515.jpg "Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)")
Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Drebrin knockout HAP1 whole cell lysate (20 μg)
Lane 3: SH-SY5Y whole cell lysate (20 μg)Lanes 1 - 3: Merged signal (red and green). Green - ab178408 observed at 120 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab178408 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. ab178408 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)
Predicted band size: 71 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--immunocytochemistry-immunofluorescence-img18048.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)")
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)
ab178408 staining Drebrin in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only.
![Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (ab178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--immunoprecipitation-img77249.jpg "Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (ab178408)")
Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (ab178408)
Immunoprecipitation. ab178408 at 1/1000 labeling Debrin in fetal brain lysate immunoprecipitated using ab178408 at 1/10.
All lanes: Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (ab178408)
Predicted band size: 71 kDa
Observed band size: 120 kDa
![Western blot - Anti-Drebrin antibody [EPR12634] (ab178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--western-blot-img103223.jpg "Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)")
Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)
All lanes: Western blot - Anti-Drebrin antibody [EPR12634] (ab178408) at 1/1000 dilution
Lane 1: Jurkat lysate at 10 µg
Lane 2: PC-12 lysate at 10 µg
Lane 3: Neuro-2a lysate at 10 µg
Lane 4: Fetal brain tissue lysate at 10 µg
Lane 5: SH-SY5Y cell lysate at 10 µg
Lane 6: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71 kDa
Observed band size: 120 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408), expandable thumbnail](https://content.abcam.com/products/images/drebrin-antibody-epr12634-ab178408--immunocytochemistry-immunofluorescence-img90392.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)")
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)
Immunofluorescent analysis of SH-SY5Y cells labeling Drebrin with ab178408 at 1/100.
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