Anti-Drebrin antibody [EPR12634] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] - BSA and Azide free (AB250035)

This data was developed using ab178408, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of SH-SY5Y cells labeling Drebrin with ab178408 at 1/100.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] - BSA and Azide free (AB250035)

This data was developed using ab178408, the same antibody clone in a different buffer formulation.

ab178408 staining Drebrin in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control : PBS only.

• WB

Supplier Data

Western blot - Anti-Drebrin antibody [EPR12634] - BSA and Azide free (AB250035)

This data was developed using ab178408, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Drebrin antibody [EPR12634] (<a href='/en-us/products/primary-antibodies/drebrin-antibody-epr12634-ab178408'>ab178408</a>) at 1/1000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

PC-12 lysate at 10 µg

Lane 3:

Neuro-2a lysate at 10 µg

Lane 4:

Fetal brain tissue lysate at 10 µg

Lane 5:

SH-SY5Y cell lysate at 10 µg

Lane 6:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 71 kDa

Observed band size: 120 kDa

false

• WB

Lab

Western blot - Anti-Drebrin antibody [EPR12634] - BSA and Azide free (AB250035)

This data was developed using ab178408, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : Drebrin knockout HAP1 whole cell lysate (20 μg)

Lane 3 : SH-SY5Y whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab178408 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab178408 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. ab178408 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Drebrin antibody [EPR12634] (<a href='/en-us/products/primary-antibodies/drebrin-antibody-epr12634-ab178408'>ab178408</a>)

Predicted band size: 71 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Drebrin antibody [EPR12634] - BSA and Azide free (AB250035)

This data was developed using ab178408, the same antibody clone in a different buffer formulation.

Immunoprecipitation. ab178408 at 1/1000 labeling Debrin in fetal brain lysate immunoprecipitated using ab178408 at 1/10.

All lanes:

Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (<a href='/en-us/products/primary-antibodies/drebrin-antibody-epr12634-ab178408'>ab178408</a>)

Predicted band size: 71 kDa

Observed band size: 120 kDa

false