JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB176318

Anti-Drebrin antibody [EPR12640(B)]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Drebrin antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

D0S117E, DBN1, Drebrin, Developmentally-regulated brain protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12640(B)] (AB176318)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12640(B)] (AB176318)

Immunofluorescent staining of SH-SY5Y cells labeling Drebrin using ab176318 at a 1/100 dilution (green) with DAPI nuclear staining (blue).

Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] (AB176318)
  • IP

Supplier Data

Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] (AB176318)

Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate labeling Drebrin using ab176318 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] (ab176318)

Predicted band size: 71 kDa

false

Western blot - Anti-Drebrin antibody [EPR12640(B)] (AB176318)
  • WB

Lab

Western blot - Anti-Drebrin antibody [EPR12640(B)] (AB176318)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : Drebrin knockout HAP1 whole cell lysate (20 μg)
Lane 3 : SH-SY5Y whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab176318 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab176318 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. ab176318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Drebrin antibody [EPR12640(B)] (ab176318)

Predicted band size: 71 kDa

false

Western blot - Anti-Drebrin antibody [EPR12640(B)] (AB176318)
  • WB

Supplier Data

Western blot - Anti-Drebrin antibody [EPR12640(B)] (AB176318)

All lanes:

Western blot - Anti-Drebrin antibody [EPR12640(B)] (ab176318) at 1/1000 dilution

Lane 1:

SH-SY5Y lysate

Lane 2:

Fetal brain lysate

Lane 3:

HeLa lysate

Lane 4:

Neuro-2a lysate

Predicted band size: 71 kDa

false

  • Carrier free

    Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12640(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB268046

Anti-Drebrin antibody [DBN1/2879]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody [DBN1/2879] (AB268046)

  • 0
  • 0
Primary Antibodies

AB60933

Anti-Drebrin antibody

KO Validated

Immunoprecipitation - Anti-Drebrin antibody (AB60933)

  • 5
  • 6
Primary Antibodies

AB60932

Anti-Drebrin antibody [mAbcam60932]

KO Validated

Flow Cytometry (Intracellular) - Anti-Drebrin antibody [mAbcam60932] (AB60932)

  • 5
  • 4
Primary Antibodies

AB178408

Anti-Drebrin antibody [EPR12634]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (AB178408)

  • 0
  • 0
Primary Antibodies

AB11068

Anti-Drebrin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (AB11068)

  • 5
  • 5

Complementary Products

Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
  • 37
Primary Antibodies

AB18258

Anti-PSD95 antibody - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody - Synaptic Marker (AB18258)

  • 5
  • 40
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections (PubMed : 20215400). Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS (PubMed : 20215400). Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity).
See full target information DBN1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40842239

2025

DHX8 regulates degradation of RNA by RNautophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ryohei Sakai,Eigo Takeda,Chihana Kabuta,Viorica Raluca Contu,Yuuki Fujiwara,Nobuhiro Fujikake,Tadafumi Hashimoto,Yoshinori Ohsumi,Tomohiro Kabuta
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com