Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (AB249903)

This data was developed using ab176318, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of SH-SY5Y cells labeling Drebrin using ab176318 at a 1/100 dilution (green), with DAPI nuclear staining (blue).

• IP

Supplier Data

Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (AB249903)

This data was developed using ab176318, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate labeling Drebrin using ab176318 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] (<a href='/en-us/products/primary-antibodies/drebrin-antibody-epr12640b-ab176318'>ab176318</a>)

Predicted band size: 71 kDa

false

• WB

Lab

Western blot - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (AB249903)

This data was developed using ab176318, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : Drebrin knockout HAP1 whole cell lysate (20 μg)

Lane 3 : SH-SY5Y whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab176318 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab176318 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. ab176318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Drebrin antibody [EPR12640(B)] (<a href='/en-us/products/primary-antibodies/drebrin-antibody-epr12640b-ab176318'>ab176318</a>)

Predicted band size: 71 kDa

false

• WB

Supplier Data

Western blot - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (AB249903)

This data was developed using ab176318, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Drebrin antibody [EPR12640(B)] (<a href='/en-us/products/primary-antibodies/drebrin-antibody-epr12640b-ab176318'>ab176318</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y lysate

Lane 2:

Fetal brain lysate

Lane 3:

HeLa lysate

Lane 4:

Neuro-2a lysate

Predicted band size: 71 kDa

false