Mouse Monoclonal DREF antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZBED1.
Images
![Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (AB277107), expandable thumbnail](https://content.abcam.com/products/images/dref-antibody-pcrp-zbed1-1e1-ab277107--immunocytochemistry-img36044.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (AB277107)")
![Flow Cytometry - Anti-DREF antibody [PCRP-ZBED1-1E1] (AB277107), expandable thumbnail](https://content.abcam.com/products/images/dref-antibody-pcrp-zbed1-1e1-ab277107--flow-cytometry-img74875.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-DREF antibody [PCRP-ZBED1-1E1] (AB277107)")
![Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (AB277107), expandable thumbnail](https://content.abcam.com/products/images/dref-antibody-pcrp-zbed1-1e1-ab277107--immunocytochemistry-img113596.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (AB277107)")
![Protein Array - Anti-DREF antibody [PCRP-ZBED1-1E1] (AB277107), expandable thumbnail](https://content.abcam.com/products/images/dref-antibody-pcrp-zbed1-1e1-ab277107--protein-array-img1989.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-DREF antibody [PCRP-ZBED1-1E1] (AB277107)")
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ZBED1. Database link O96006
Reactivity data
Select an application| ICC | Flow Cyt | Protein Array | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA (PubMed:27068747). This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12 (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747). The resulting increased transcriptional activity drives cell proliferation (PubMed:12663651, PubMed:17220279). Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747).(Microbial infection) Binds to human adenovirus gene promoters and contributes to transcriptional repression and virus growth inhibition during early stages of infection.
Alternative names
E3 SUMO-protein ligase ZBED1, DNA replication-related element-binding factor, Putative Ac-like transposable element, Zinc finger BED domain-containing protein 1, dREF homolog, KIAA0785, ZBED1, TRAMP, hDREF, DREF, ALTE
Recommended products
Mouse Monoclonal DREF antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZBED1.
Alternative names
E3 SUMO-protein ligase ZBED1, DNA replication-related element-binding factor, Putative Ac-like transposable element, Zinc finger BED domain-containing protein 1, dREF homolog, KIAA0785, ZBED1, TRAMP, hDREF, DREF, ALTE
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ZBED1. Database link O96006
- Clone number
PCRP-ZBED1-1E1
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from bioreactor concentrate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
DREF also known as DNA replication-related element-binding factor is a transcription factor that regulates gene expression associated with DNA replication and cell proliferation. It is a nuclear protein with a mass of approximately 75 kDa. DREF is ubiquitously expressed in various tissues indicating its broad role in cellular processes. It binds specifically to DNA replication-related elements within the promoter regions of its target genes enabling the initiation of transcription important for cell growth and division.
- Biological function summary
DREF plays an important role in regulating the expression of genes involved in DNA replication and cell cycle progression. It is known to interact with other proteins to form complexes that influence gene transcription. Through these interactions DREF coordinates the precise timing of DNA replication initiation ensuring proper cell cycle progression and maintaining genomic stability. It interacts with factors like TBP (TATA-binding protein) and contributes to the assembly of transcription machinery at replication-related promoters.
- Pathways
DREF is integral to the regulation of the cell cycle and DNA replication pathways. It directly activates genes necessary for the S-phase entry. DREF interacts with the E2F family of transcription factors which also control cell cycle progression and apoptosis. The E2F/DREF pathway ensures balanced cell proliferation and programmed cell death preventing uncontrolled cell growth. DREF's role in DNA replication and cell cycle highlights its importance in maintaining cellular homeostasis.
- Associated diseases and disorders
Mutations or dysregulation of DREF can be linked to cancer and developmental disorders. Overexpression of DREF may lead to unregulated cell proliferation contributing to tumorigenesis. It associates with the p53 protein known for its role in mediating DNA repair and apoptosis following DNA damage. Dysregulation of the DREF and p53 interaction can affect the cell's response to DNA damage thereby influencing cancer progression. Understanding DREF's function in these contexts aids in developing potential therapeutic strategies targeting its pathway.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107), expandable thumbnail](https://content.abcam.com/products/images/dref-antibody-pcrp-zbed1-1e1-ab277107--immunocytochemistry-img36044.jpg "Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107)")
Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107)
Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling DREF with ab277107 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
![Flow Cytometry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107), expandable thumbnail](https://content.abcam.com/products/images/dref-antibody-pcrp-zbed1-1e1-ab277107--flow-cytometry-img74875.jpg "Flow Cytometry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107)")
Flow Cytometry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107)
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling DREF using ab277107 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
![Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107), expandable thumbnail](https://content.abcam.com/products/images/dref-antibody-pcrp-zbed1-1e1-ab277107--immunocytochemistry-img113596.jpg "Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107)")
Immunocytochemistry - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107)
Immunofluorescence analysis of PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells labeling DREF with ab277107 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
![Protein Array - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107), expandable thumbnail](https://content.abcam.com/products/images/dref-antibody-pcrp-zbed1-1e1-ab277107--protein-array-img1989.jpg "Protein Array - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107)")
Protein Array - Anti-DREF antibody [PCRP-ZBED1-1E1] (ab277107)
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277107. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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