Rabbit Polyclonal DRIM antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human UTP20 aa 2700 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UTP20 aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75691
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Bat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Chimpanzee, Rhesus monkey, Bat, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Chimpanzee, Rhesus monkey, Bat, Elephant | Dilution info - | Notes - |
Target data
Function
Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in 18S pre-rRNA processing. Associates with U3 snoRNA.
Alternative names
DRIM, UTP20, Small subunit processome component 20 homolog, Down-regulated in metastasis protein, Novel nucleolar protein 73, Protein Key-1A6, NNP73
Recommended products
Rabbit Polyclonal DRIM antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human UTP20 aa 2700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UTP20 aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75691
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab86093 was affinity purified using an epitope specific to DRIM immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target 'DRIM' also known as Disease Resistance Interactor M is a protein involved in various cellular activities. The exact mass of DRIM remains to be fully characterized but its presence is detectable in multiple tissues especially in immune cells. Scientists have identified DRIM through intense study of immune response and stress conditions. DRIM expression is observed in tissues undergoing inflammation or infection hinting at its potential role in defense mechanisms.
- Biological function summary
DRIM influences cellular responses that modulate immune defense and stress tolerance. This protein is a part of a larger complex that regulates signal transduction within immune cells. Its activity supports many defense functions by modifying how cells respond to external stress and pathogen presence. Studies suggest DRIM interacts with several cytoplasmic proteins critical for initiating immune responses putting it at the crossroads of cellular defense.
- Pathways
DRIM plays a part in inflammatory and stress response pathways. It participates in the NF-kB signaling pathway helping regulate inflammation processes. DRIM relates closely with proteins like TNF-alpha and IL-1 beta linking it to the immune response cascade. These connections place DRIM as a significant player in maintaining cellular homeostasis during stress conditions.
- Associated diseases and disorders
DRIM shows relevance to autoimmune diseases and chronic inflammatory disorders. DRIM's involvement in the NF-kB pathway ties it to conditions like rheumatoid arthritis and systemic lupus erythematosus. Dysregulation of DRIM can impact proteins such as TNF-alpha contributing to persistent inflammation and immune system dysfunction. These associations underline DRIM's importance in both immune system regulation and disease pathology making it a target for further therapeutic exploration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-DRIM antibody (ab86093)
All lanes: Western blot - Anti-DRIM antibody (ab86093) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Predicted band size: 318 kDa
Observed band size: 318 kDa, 65 kDa
Exposure time: 30s
Immunoprecipitation - Anti-DRIM antibody (ab86093)
Detection of DRIM by Western Blot of Immunprecipitate.
ab86093 at 1µg/ml staining DRIM in HeLa whole cell lysate immunoprecipitated using ab86093 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-DRIM antibody (ab86093)
Predicted band size: 318 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com