AB86093

Anti-DRIM antibody

Name: Anti-DRIM antibody (ab86093) | Abcam
Brand: Abcam Limited
SKU: AB86093

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(1 Publication)

Rabbit Polyclonal DRIM antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UTP20 aa 2700 to C-terminus.

View Alternative Names

DRIM, UTP20, Small subunit processome component 20 homolog, Down-regulated in metastasis protein, Novel nucleolar protein 73, Protein Key-1A6, NNP73

2 Images

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Immunoprecipitation - Anti-DRIM antibody (AB86093)

Detection of DRIM by Western Blot of Immunprecipitate.
ab86093 at 1µg/ml staining DRIM in HeLa whole cell lysate immunoprecipitated using ab86093 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-DRIM antibody (ab86093)

Predicted band size: 318 kDa

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Western blot - Anti-DRIM antibody (AB86093)

All lanes:

Western blot - Anti-DRIM antibody (ab86093) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 318 kDa

Observed band size: 318 kDa,65 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human UTP20 aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75691

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab86093 was affinity purified using an epitope specific to DRIM immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 'DRIM' also known as Disease Resistance Interactor M is a protein involved in various cellular activities. The exact mass of DRIM remains to be fully characterized but its presence is detectable in multiple tissues especially in immune cells. Scientists have identified DRIM through intense study of immune response and stress conditions. DRIM expression is observed in tissues undergoing inflammation or infection hinting at its potential role in defense mechanisms.

Biological function summary

DRIM influences cellular responses that modulate immune defense and stress tolerance. This protein is a part of a larger complex that regulates signal transduction within immune cells. Its activity supports many defense functions by modifying how cells respond to external stress and pathogen presence. Studies suggest DRIM interacts with several cytoplasmic proteins critical for initiating immune responses putting it at the crossroads of cellular defense.

Pathways

DRIM plays a part in inflammatory and stress response pathways. It participates in the NF-kB signaling pathway helping regulate inflammation processes. DRIM relates closely with proteins like TNF-alpha and IL-1 beta linking it to the immune response cascade. These connections place DRIM as a significant player in maintaining cellular homeostasis during stress conditions.

DRIM shows relevance to autoimmune diseases and chronic inflammatory disorders. DRIM's involvement in the NF-kB pathway ties it to conditions like rheumatoid arthritis and systemic lupus erythematosus. Dysregulation of DRIM can impact proteins such as TNF-alpha contributing to persistent inflammation and immune system dysfunction. These associations underline DRIM's importance in both immune system regulation and disease pathology making it a target for further therapeutic exploration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in 18S pre-rRNA processing. Associates with U3 snoRNA.

See full target information UTP20

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental eye research 148:12-23 PubMed27170050

2016

Primate neural retina upregulates IL-6 and IL-10 in response to a herpes simplex vector suggesting the presence of a pro-/anti-inflammatory axis.

Applications

Unspecified application

Species

Unspecified reactive species

Monica M Sauter,Curtis R Brandt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DRIM antibody

Immunoprecipitation - Anti-DRIM antibody (AB86093)

Western blot - Anti-DRIM antibody (AB86093)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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