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Rabbit Recombinant Monoclonal DRIP130 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-DRIP130 antibody [EPR17418] (AB200351), expandable thumbnail
  • Western blot - Anti-DRIP130 antibody [EPR17418] (AB200351), expandable thumbnail
  • Western blot - Anti-DRIP130 antibody [EPR17418] (AB200351), expandable thumbnail
  • Western blot - Anti-DRIP130 antibody [EPR17418] (AB200351), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRIP130 antibody [EPR17418] (AB200351), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Required for transcriptional activation subsequent to the assembly of the pre-initiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.

Alternative names

ARC130, CRSP3, DRIP130, KIAA1216, SUR2, MED23, Mediator of RNA polymerase II transcription subunit 23, Activator-recruited cofactor 130 kDa component, Cofactor required for Sp1 transcriptional activation subunit 3, Mediator complex subunit 23, Protein sur-2 homolog, Transcriptional coactivator CRSP130, Vitamin D3 receptor-interacting protein complex 130 kDa component, CRSP complex subunit 3, hSur-2

Recommended products

Rabbit Recombinant Monoclonal DRIP130 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17418
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DRIP130 also known as MED23 is a component of the Mediator complex. It plays a significant role as a transcriptional co-activator for RNA polymerase II transcription. DRIP130 has a molecular mass of approximately 130 kDa. Expression of DRIP130 occurs in various tissues more notably in the liver and heart indicating its widespread biological roles in the body.

Biological function summary

DRIP130 integrates signals from activators and repressors to facilitate transcription regulation. It is a part of the larger Mediator complex which is essential for conveying instructions from transcription factors to RNA polymerase II. By acting at promoters DRIP130 helps modulate gene expression impacting processes like cell growth and differentiation. Its function within this multi-subunit complex emphasizes its importance in cellular communication and transcriptional regulation.

Pathways

DRIP130 contributes to the MAPK/ERK signaling pathway and the Wnt signaling pathway. Within these pathways it interacts with proteins such as MAPK and β-catenin to influence cell cycle progression and cellular responses. These pathways are critical for controlling growth and maintaining cellular functions indicating DRIP130's vital role in pathway integration and cellular homeostasis.

Associated diseases and disorders

DRIP130 has connections to cancer and neurological disorders. Alterations in DRIP130 expression or function can lead to aberrant cell proliferation contributing to cancer development. Moreover DRIP130's interaction with proteins like β-catenin in the Wnt pathway may influence neurological conditions by affecting neural cell signaling or development. Understanding these links provides insight into potential therapeutic targets for managing such disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351), expandable thumbnail

    Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351) at 1/10000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

    Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 3: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 156 kDa

    Observed band size: 130 kDa

    Exposure time: 3min

  • Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351), expandable thumbnail

    Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 156 kDa

    Observed band size: 130 kDa

    Exposure time: 3min

  • Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351), expandable thumbnail

    Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351) at 1/1000 dilution

    Lane 1: Mouse kidney lysate at 10 µg

    Lane 2: Mouse spleen lysate at 10 µg

    Lane 3: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 4: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate at 10 µg

    Lane 5: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 6: NIH/3T3 (Mouse embyro fibroblast cells ) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 156 kDa

    Observed band size: 130 kDa

    Exposure time: 15s

  • Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351), expandable thumbnail

    Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351)

    The observed MW is consistent with what has been described in the literature (PMID: 12242338).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-DRIP130 antibody [EPR17418] (ab200351) at 1/1000 dilution

    All lanes: Rat kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 156 kDa

    Observed band size: 130 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRIP130 antibody [EPR17418] (ab200351), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRIP130 antibody [EPR17418] (ab200351)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling DRIP130 with ab200351 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRIP130 antibody [EPR17418] (ab200351), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRIP130 antibody [EPR17418] (ab200351)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling DRIP130 with ab200351 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DRIP130 antibody [EPR17418] (ab200351), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DRIP130 antibody [EPR17418] (ab200351)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling DRIP130 with ab200351 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200351 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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