Rabbit Polyclonal Drosha antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 95 publications.
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- Science advances 9:eadf05972023The Integrator complex regulates microRNA abundance through RISC loading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNina Kirstein,Sadat Dokaneheifard,Pradeep Reddy Cingaram,Monica Guiselle Valencia,Felipe Beckedorff,Helena Gomes Dos Santos,Ezra Blumenthal,Mina Masoumeh Tayari,Gabriel Stephen Gaidosh,Ramin ShiekhattarPubMed 36763664
- Viruses 15:2023Oncolytic Measles Virus Encoding MicroRNA for Targeted RNA Interference.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie C Anker,Marie G Szczeponik,Jan Dessila,Katia Dittus,Christine E Engeland,Dirk Jäger,Guy Ungerechts,Mathias F LeberPubMed 36851522
- Aging 14:6520-65362022Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTung-Wei Hsu,Hsin-An Chen,Po-Hsiang Liao,Yen-Hao Su,Ching-Feng Chiu,Chih-Yang Huang,Yu-Jung Lin,Chih-Chiang Hung,Ming-Hsin Yeh,Shian-Ying Sung,Chih-Ming SuPubMed 35951366
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e224122022The hypoxia-induced changes in miRNA-mRNA in RNA-induced silencing complexes and HIF-2 induced miRNAs in human endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrianna Moszyńska,Maciej Jaśkiewicz,Marcin Serocki,Aleksandra Cabaj,David K Crossman,Sylwia Bartoszewska,Magdalena Gebert,Michał Dąbrowski,James F Collawn,Rafal BartoszewskiPubMed 35713587
- Cancer science 113:1601-16122022Transcriptional suppression of Dicer by HOXB-AS3/EZH2 complex dictates sorafenib resistance and cancer stemness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi-Feng Tseng,Li-Tzong Chen,Horng-Dar Wang,Yi-Hong Liu,Shine-Gwo ShiahPubMed 35253323
- Nucleic acids research 49:2522-25362021Novel roles of an intragenic G-quadruplex in controlling microRNA expression and cardiac function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Zhu,Juan Gao,Xian-Juan Lin,Yun-Yun Gong,Yan-Chao Qi,Yuan-Liang Ma,Yuan-Xiu Song,Wei Tan,Fang-Yuan Li,Min Ye,Jun Gong,Qing-Hua Cui,Zeng-Hui Huang,You-Yi Zhang,Xiu-Jie Wang,Feng Lan,Shi-Qiang Wang,Gu Yuan,Yue Feng,Ming XuPubMed 33561291
- Journal of cellular physiology 236:4420-44342020ERK-mediated transcriptional activation of Dicer is involved in gemcitabine resistance of pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYen-Hao Su,Tung-Wei Hsu,Hsin-An Chen,Chih-Ming Su,Ming-Te Huang,Ta-Hsien Chuang,J Leo Su,Chia-Ling Hsieh,Ching-Feng ChiuPubMed 33184874
- RNA biology :1-112020Tissue-specific and transcription-dependent mechanisms regulate primary microRNA processing efficiency of the human chromosome 19 MicroRNA cluster.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÁbel Fóthi et. al.PubMed 33052778
- Theranostics 10:8281-82972020RNA helicase p68 inhibits the transcription and post-transcription of in ADPKD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Zhang et. al.PubMed 32724471
- Journal of dermatological science 98:186-1942020Dysregulation of microRNA expression in diabetic skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnke Baldini et. al.PubMed 32402513
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Tested |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
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Target data
Function
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons (By similarity).
Alternative names
RN3, RNASE3L, RNASEN, DROSHA, Ribonuclease 3, Protein Drosha, Ribonuclease III, p241, RNase III
Recommended products
Rabbit Polyclonal Drosha antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 95 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Three isoforms of 159, 156 and 143 kDa.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
This image is courtesy of Ahmad M. Khalil and Daniel J. Driscoll, University of Florida College of Medicine Genetics Institute.Immunocytochemistry - Anti-Drosha antibody (ab12286)
Human female amniocytes immunostained with ab12286 Drosha (FITC) (1/25 dilution). The DNA is labelled red with propidium iodide. This image was submitted as part of a review by Ahmad Khalil.
Immunocytochemistry - Anti-Drosha antibody (ab12286)
ab12286 at a 1/25 dilution staining human HeLa cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Cy3 conjugated donkey anti-rabbit polyclonal antibody.
This image is courtesy of an Abreview submitted on 7 February 2006.
Immunocytochemistry - Anti-Drosha antibody (ab12286)
ICC/IF image of ab12286 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12286, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunocytochemistry - Anti-Drosha antibody (ab12286)
ICC/IF image of ab12286 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12886, 5μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-Drosha antibody (ab12286)
All lanes: Western blot - Anti-Drosha antibody (ab12286) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDa
Western blot - Anti-Drosha antibody (ab12286)
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-Drosha antibody (ab12286) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 115 kDa, 170 kDa
Exposure time: 12min
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