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AB245398

Anti-Drosha antibody

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Rabbit Polyclonal Drosha antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human DROSHA aa 1300 to C-terminus.

View Alternative Names

RN3, RNASE3L, RNASEN, DROSHA, Ribonuclease 3, Protein Drosha, Ribonuclease III, p241, RNase III

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Immunoprecipitation - Anti-Drosha antibody (AB245398)
  • IP

Supplier Data

Immunoprecipitation - Anti-Drosha antibody (AB245398)

Drosha was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245398 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab245398 at 1 μg/ml.

Lane 1 : ab245398 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-Drosha antibody (ab245398)

Predicted band size: 159 kDa

false

Western blot - Anti-Drosha antibody (AB245398)
  • WB

Unknown

Western blot - Anti-Drosha antibody (AB245398)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-Drosha antibody (ab245398) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 159 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human DROSHA aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NRR4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245398 was affinity purified using an epitope specific to Drosha immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons (By similarity).
See full target information DROSHA
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Product promise

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