Rabbit Recombinant Monoclonal Drosha antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--immunocytochemistry-immunofluorescence-img19721.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)")
![Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--western-blot-img10536.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)")
![Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--western-blot-img7002.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)")
![Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--flow-cytometry-intracellular-img38676.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)")
![Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--immunocytochemistry-immunofluorescence-img24718.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Target data
Function
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons (By similarity).
Alternative names
RN3, RNASE3L, RNASEN, DROSHA, Ribonuclease 3, Protein Drosha, Ribonuclease III, p241, RNase III
Recommended products
Rabbit Recombinant Monoclonal Drosha antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23046-123
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab267793 is the carrier-free version of Anti-Drosha antibody [EPR23046-123] ab242147.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--immunocytochemistry-immunofluorescence-img19721.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)")
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Drosha with Anti-Drosha antibody [EPR23046-123] ab242147 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in NIH/3T3 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Drosha antibody [EPR23046-123] ab242147).
![Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--western-blot-img10536.jpg "Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)")
Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Drosha antibody [EPR23046-123] ab242147).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Weakly reactivity with undetermined proteins in HeLa.
Exposure time: 3 minutes
All lanes: Western blot - Anti-Drosha antibody [EPR23046-123] (Anti-Drosha antibody [EPR23046-123] ab242147) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
Lane 2: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 159 kDa
Observed band size: 158 kDa
Exposure time: 3min
![Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--western-blot-img7002.jpg "Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)")
Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Drosha antibody [EPR23046-123] ab242147).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 48 seconds.
All lanes: Western blot - Anti-Drosha antibody [EPR23046-123] (Anti-Drosha antibody [EPR23046-123] ab242147) at 1/1000 dilution
Lane 1: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 3: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 159 kDa
Observed band size: 158 kDa
Exposure time: 48s
![Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--flow-cytometry-intracellular-img38676.jpg "Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)")
Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Drosha with Anti-Drosha antibody [EPR23046-123] ab242147 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Drosha antibody [EPR23046-123] ab242147).
![Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--immunocytochemistry-immunofluorescence-img24718.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)")
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw264.7 cells labelling Drosha with Anti-Drosha antibody [EPR23046-123] ab242147 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in Raw264.7 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Drosha antibody [EPR23046-123] ab242147).
![Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--flow-cytometry-intracellular-img33747.jpg "Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)")
Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Drosha with Anti-Drosha antibody [EPR23046-123] ab242147 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Drosha antibody [EPR23046-123] ab242147).
![Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793), expandable thumbnail](https://content.abcam.com/products/images/drosha-antibody-epr23046-123-bsa-and-azide-free-ab267793--flow-cytometry-intracellular-img33747.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (ab267793)")
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