Anti-Drosha antibody [EPR23046-123] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Drosha with ab242147 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242147).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Drosha with ab242147 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242147).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw264.7 cells labelling Drosha with ab242147 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in Raw264.7 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242147).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Drosha with ab242147 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242147).

• WB

Unknown

Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242147).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Weakly reactivity with undetermined proteins in HeLa.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Drosha antibody [EPR23046-123] (<a href='/en-us/products/primary-antibodies/drosha-antibody-epr23046-123-ab242147'>ab242147</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 2:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 159 kDa

Observed band size: 158 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Anti-Drosha antibody [EPR23046-123] - BSA and Azide free (AB267793)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242147).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-Drosha antibody [EPR23046-123] (<a href='/en-us/products/primary-antibodies/drosha-antibody-epr23046-123-ab242147'>ab242147</a>) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 3:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 159 kDa

Observed band size: 158 kDa

false

Exposure time: 48s