Anti-DRP1 antibody [EPR19274] ab184247 is a rabbit monoclonal antibody that is used in DRP1 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR19274 is cited in over 220 publications
- One antibody for all your DRP1 staining, use in DRP1 western blotting, IHC, immunofluorescence and flow cytometry
New 20 ul size available
IgG
Rabbit
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Expected | Expected |
Mouse | Expected | Tested | Tested | Tested | Tested |
Rat | Expected | Tested | Expected | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/250 | Notes - |
Species Human | Dilution info 1/250 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/70 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes IHC is recommended for rat and mouse only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes IHC is recommended for rat and mouse only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Functions in mitochondrial and peroxisomal division (PubMed:11514614, PubMed:12499366, PubMed:17301055, PubMed:17460227, PubMed:17553808, PubMed:18695047, PubMed:18838687, PubMed:19342591, PubMed:19411255, PubMed:19638400, PubMed:23283981, PubMed:23530241, PubMed:23921378, PubMed:26992161, PubMed:27145208, PubMed:27145933, PubMed:27301544, PubMed:27328748, PubMed:29478834, PubMed:32439975, PubMed:32484300, PubMed:9570752, PubMed:9786947). Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism (PubMed:23530241, PubMed:23584531, PubMed:33850055). The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes (PubMed:23283981, PubMed:23921378, PubMed:29899447). While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane (PubMed:29899447). Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner (PubMed:32484300). Plays an important role in mitochondrial fission during mitosis (PubMed:19411255, PubMed:26992161, PubMed:27301544, PubMed:27328748). Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage (By similarity). Required for normal brain development, including that of cerebellum (PubMed:17460227, PubMed:26992161, PubMed:27145208, PubMed:27301544, PubMed:27328748). Facilitates developmentally regulated apoptosis during neural tube formation (By similarity). Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues (By similarity). Required for formation of endocytic vesicles (PubMed:20688057, PubMed:23792689, PubMed:9570752). Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles (PubMed:17015472, PubMed:23792689). Required for programmed necrosis execution (PubMed:22265414). Rhythmic control of its activity following phosphorylation at Ser-637 is essential for the circadian control of mitochondrial ATP production (PubMed:29478834).Isoform 1Inhibits peroxisomal division when overexpressed.Isoform 4Inhibits peroxisomal division when overexpressed.
DLP1, DRP1, DRP1, DNM1L, DLP1, Dynamin-1-like protein, Dnm1p/Vps1p-like protein, Dynamin family member proline-rich carboxyl-terminal domain less, Dynamin-like protein, Dynamin-like protein 4, Dynamin-like protein IV, Dynamin-related protein 1, DVLP, Dymple, HdynIV
Anti-DRP1 antibody [EPR19274] ab184247 is a rabbit monoclonal antibody that is used in DRP1 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR19274 is cited in over 220 publications
- One antibody for all your DRP1 staining, use in DRP1 western blotting, IHC, immunofluorescence and flow cytometry
New 20 ul size available
IgG
Rabbit
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR19274
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The DRP1 protein also known as Dynamin-Related Protein 1 has a molecular weight of approximately 80 kDa. DRP1 is a GTPase that plays a central role in mitochondrial fission. The protein is widely expressed in many tissues with high levels in the brain heart and skeletal muscles. It functionally interacts with other proteins on the mitochondrial membrane to drive the division of mitochondria. As a mechanism DRP1 assembles into ring-like structures around constriction points on the outer mitochondrial membrane facilitating membrane scission.
DRP1 regulates mitochondrial morphology by controlling mitochondrial division. This process is essential in maintaining the balance between mitochondrial fission and fusion which affects mitochondrial distribution and function. DRP1 also associates with protein complexes involved in this dynamic balance including MFF (mitochondrial fission factor) and FIS1 (fission 1 protein). Phosphorylation state of DRP1 influences its activity; different phosphorylation sites either activate or inhibit its function.
The mechanical action of DRP1 integrates closely into the mitochondrial quality control and apoptosis pathways. Mitochondrial fission driven by DRP1 is necessary for the removal of damaged mitochondria through mitophagy. It also influences the apoptotic pathway where DRP1 translocates to mitochondria under pro-apoptotic signals often in interaction with proteins such as Bax and Bak that promote cytochrome c release. This relationship affects cell survival and energy homeostasis.
Improper regulation of DRP1 is linked to neurodegenerative diseases like Alzheimer's disease and Parkinson's disease. Altered DRP1 activity or expression disrupts mitochondrial homeostasis contributing to neuronal cell death pathways. Connections with proteins such as Tau in Alzheimer's and Parkin in Parkinson's influence the progression of these disorders. Additionally DRP1's role in cardiac disorders highlights its importance in maintaining normal cardiac function through mitochondrial regulation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 30 seconds; Lane 4,5 and 6: 8 seconds.
DRP1 can be SUMOylated, as described in the literature (PMID: 19638400).
All lanes: Western blot - Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution
Lane 1: A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg
Lane 2: U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 10 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg
Lane 5: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg
Lane 6: HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 82 kDa
Observed band size: 83 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DRP1 with ab184247 at 1/250 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR19274] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab184247 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling DRP1 with ab184247 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal -Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution
All lanes: Human fetal kidney lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 53 kDa, 56 kDa, 82 kDa
Observed band size: 53 kDa, 56 kDa, 83 kDa
Exposure time: 30s
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: 2 seconds; Lane 2: 8 seconds; Lane 3: 3 seconds.
All lanes: Western blot - Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution
Lane 1: Rat brain lysate at 10 µg
Lane 2: Rat heart lysate at 10 µg
Lane 3: Mouse brain lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 82 kDa
Observed band size: 83 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution
Lane 1: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 141 kDa, 42 kDa, 82 kDa, 86 kDa, 94 kDa
Observed band size: 83 kDa, 86 kDa
Exposure time: 3min
DRP1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184247 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184247 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10μg (Input).
Lane 2: ab184247 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR19274]-Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184247 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Note: DRP1 can be SUMOylated, as described in the literature (PMID: 19638400).
All lanes: Immunoprecipitation - Anti-DRP1 antibody [EPR19274] (ab184247)
Predicted band size: 82 kDa
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DRP1 with ab184247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse cerebrum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling DRP1 with ab184247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on rat cerebellum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling DRP1 with ab184247 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR19274] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab184247 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation experiments were performed with an anti-6X His tag® antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) at 1:5000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.
All lanes: Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (Anti-DRP1 (phospho S616) antibody [EPR27387-57] ab314755) at 1/1000 dilution
Lane 1: Untreated 293T cells transfected with a human DRP1 expression vector containi a myc-His-tag® lysate at 20 µg
Lane 2: 293T cells transfected with a human DRP1 expression vector containing a myc-His-tag®. Overexpressi cells were treated with 100/ml nocodazole for 17 hours whole cell lysate at 20 µg
Lane 3: Untreated 293T cells transfected with a human DRP1(S616A mutant ) expression vector containi a myc-His-tag® lysate at 20 µg
Lane 4: 293T cells transfected with a human DRP1(S616A mutant) expression vector containing a myc-His-tag®. Overexpressi cells were treated with 100/ml nocodazole for 17 hours whole cell lysate at 20 µg
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 83 kDa
Exposure time: 3s
Blocking and diluting buffer: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control at 1/1000000 dilution.
Anti-DRP1 antibody [EPR19275] ab184248 worked better in western blot application.
Lanes 1 - 4: Western blot - Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution
Lanes 5 - 8: Western blot - Anti-DRP1 antibody [EPR19275] (Anti-DRP1 antibody [EPR19275] ab184248) at 1/1000 dilution
Lanes 1 and 5: Mouse brain tissue lysate at 20 µg
Lanes 2 and 6: Mouse liver tissue lysate at 20 µg
Lanes 3 and 7: Mouse heart tissue lysate at 20 µg
Lanes 4 and 8: Mouse lung tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 80 kDa
Exposure time: 7s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 20 kDa is unknown.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19436752).
ab184247 was used as the loading control at a 1/1000 dilution.
All lanes: Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (Anti-DRP1 (phospho S616) antibody [EPR27387-57] ab314755) at 1/1000 dilution
Lane 1: Untreated HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (untreated membrane) at 20 µg
Lane 2: HAP1 treated with 100 ng/ml nocodazole for 16 hours whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (phosphatase treated membrane) at 20 µg
Lane 4: HAP1 treated with 100 ng/ml nocodazole for 16 hours whole cell lysate (phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 82 kDa
Observed band size: 83 kDa
Exposure time: 92s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.
All lanes: Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (Anti-DRP1 (phospho S616) antibody [EPR27387-57] ab314755) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg
Lane 2: PC-12 whole cell lysate (phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 83 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 20 kDa (in lane 2) is unknown. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.
All lanes: Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (Anti-DRP1 (phospho S616) antibody [EPR27387-57] ab314755) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: NIH/3T3 treated with /ml nocodazole for 17 hours whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 83 kDa
Exposure time: 180s
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