Anti-DRP1 antibody [EPR19275]
- 20ul selling size
- RabMAb
- Recombinant
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(11 Publications)
Rabbit Recombinant Monoclonal DRP1 antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
View Alternative Names
DLP1, DRP1, DNM1L, Dynamin-1-like protein, Dnm1p/Vps1p-like protein, Dynamin family member proline-rich carboxyl-terminal domain less, Dynamin-like protein, Dynamin-like protein 4, Dynamin-like protein IV, Dynamin-related protein 1, DVLP, Dymple, HdynIV
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (AB184248)
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling DRP1 with ab184248 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human glioma [PMID : 25730670]. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (AB184248)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human cerebellum [PMID : 9422767]. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-DRP1 antibody [EPR19275] (AB184248)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DRP1 with ab184248 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
- IP
Supplier Data
Immunoprecipitation - Anti-DRP1 antibody [EPR19275] (AB184248)
DRP1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184248 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184248 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HeLa whole cell lysate, 10 μg (Input).
Lane 2 : ab184248 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184248 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.
All lanes:
Immunoprecipitation - Anti-DRP1 antibody [EPR19275] (ab184248)
Predicted band size: 82 kDa
Observed band size: 83 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (AB184248)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebellum [PMID : 9422767]. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] (AB184248)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebellum [PMID : 9422767]. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-DRP1 antibody [EPR19275] (AB184248)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure times : Lane 1-8 : 3 minutes; Lane 9 : 30 seconds.
All lanes:
Western blot - Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution
Lane 1:
A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3:
Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 4:
HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 5:
HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 6:
HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 7:
PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 8:
NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
Lane 9:
U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 82 kDa
Observed band size: 83 kDa
false
- WB
Supplier Data
Western blot - Anti-DRP1 antibody [EPR19275] (AB184248)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution
Lane 1:
Human fetal heart lysate at 10 µg
Lane 2:
Human fetal kidney lysate at 10 µg
Secondary
All lanes:
Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution
Predicted band size: 82 kDa
Observed band size: 83 kDa
false
Exposure time: 30s
- WB
Supplier Data
Western blot - Anti-DRP1 antibody [EPR19275] (AB184248)
Blocking and diluting buffer : 5% NFDM/TBST.
ab181602 was used as a loading control at 1/1000000 dilution.
ab184248 worked better in western blot application.
Lanes 1 - 4:
Western blot - Anti-DRP1 antibody [EPR19274] (<a href='/en-us/products/primary-antibodies/drp1-antibody-epr19274-ab184247'>ab184247</a>) at 1/1000 dilution
Lanes 5 - 8:
Western blot - Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution
Lanes 1 and 5:
Mouse brain tissue lysate at 20 µg
Lanes 2 and 6:
Mouse liver tissue lysate at 20 µg
Lanes 3 and 7:
Mouse heart tissue lysate at 20 µg
Lanes 4 and 8:
Mouse lung tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 80 kDa
false
Exposure time: 7s
- WB
Supplier Data
Western blot - Anti-DRP1 antibody [EPR19275] (AB184248)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure times : Lane 1 : 1 second; Lane 2 : 3 seconds; Lane 3 : 10 seconds; Lane 4 : 1 second; Lane 5 : 5 seconds; Lane 6 : 5 seconds.
All lanes:
Western blot - Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution
Lane 1:
Mouse brain tissue lysate at 10 µg
Lane 2:
Mouse heart tissue lysate at 10 µg
Lane 3:
Mouse spleen tissue lysate at 10 µg
Lane 4:
Rat brain tissue lysate at 10 µg
Lane 5:
Rat heart tissue lysate at 10 µg
Lane 6:
Rat spleen tissue lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 82 kDa
Observed band size: 83 kDa
false
Related conjugates and formulations (1)
-
Anti-DRP1 antibody [EPR19275] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DRP1 regulates mitochondrial morphology by controlling mitochondrial division. This process is essential in maintaining the balance between mitochondrial fission and fusion which affects mitochondrial distribution and function. DRP1 also associates with protein complexes involved in this dynamic balance including MFF (mitochondrial fission factor) and FIS1 (fission 1 protein). Phosphorylation state of DRP1 influences its activity; different phosphorylation sites either activate or inhibit its function.
Pathways
The mechanical action of DRP1 integrates closely into the mitochondrial quality control and apoptosis pathways. Mitochondrial fission driven by DRP1 is necessary for the removal of damaged mitochondria through mitophagy. It also influences the apoptotic pathway where DRP1 translocates to mitochondria under pro-apoptotic signals often in interaction with proteins such as Bax and Bak that promote cytochrome c release. This relationship affects cell survival and energy homeostasis.
Product protocols
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Target data
Publications (11)
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Pharmaceutical biology 63:188-200 PubMed40188381
2025
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Journal of biochemical and molecular toxicology 38:e70064 PubMed39569601
2024
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Shock (Augusta, Ga.) 60:100-109 PubMed37141173
2023
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Molecular brain 16:36 PubMed37098623
2023
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Aging 15:276-286 PubMed36626245
2023
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International journal of molecular sciences 23: PubMed36012573
2022
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Cells 10: PubMed33924585
2021
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Toxins 12: PubMed31936883
2020
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Experimental and therapeutic medicine 18:4113-4119 PubMed31611942
2019
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Life sciences 232:116561 PubMed31247208
2019
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com