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Rabbit Recombinant Monoclonal DRP1 phospho S616 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide samples.

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Images

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755), expandable thumbnail
  • Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755), expandable thumbnail
  • Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755), expandable thumbnail
  • Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755), expandable thumbnail
  • Dot Blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotIHC-PFlow Cyt (Intra)IPICC/IF
Human
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Functions in mitochondrial and peroxisomal division (PubMed:11514614, PubMed:12499366, PubMed:17301055, PubMed:17460227, PubMed:17553808, PubMed:18695047, PubMed:18838687, PubMed:19342591, PubMed:19411255, PubMed:19638400, PubMed:23283981, PubMed:23530241, PubMed:23921378, PubMed:26992161, PubMed:27145208, PubMed:27145933, PubMed:27301544, PubMed:27328748, PubMed:29478834, PubMed:32439975, PubMed:32484300, PubMed:9570752, PubMed:9786947). Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism (PubMed:23530241, PubMed:23584531, PubMed:33850055). The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes (PubMed:23283981, PubMed:23921378, PubMed:29899447). While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane (PubMed:29899447). Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner (PubMed:32484300). Plays an important role in mitochondrial fission during mitosis (PubMed:19411255, PubMed:26992161, PubMed:27301544, PubMed:27328748). Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage (By similarity). Required for normal brain development, including that of cerebellum (PubMed:17460227, PubMed:26992161, PubMed:27145208, PubMed:27301544, PubMed:27328748). Facilitates developmentally regulated apoptosis during neural tube formation (By similarity). Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues (By similarity). Required for formation of endocytic vesicles (PubMed:20688057, PubMed:23792689, PubMed:9570752). Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles (PubMed:17015472, PubMed:23792689). Required for programmed necrosis execution (PubMed:22265414). Rhythmic control of its activity following phosphorylation at Ser-637 is essential for the circadian control of mitochondrial ATP production (PubMed:29478834). Isoform 1. Inhibits peroxisomal division when overexpressed. Isoform 4. Inhibits peroxisomal division when overexpressed.

Alternative names

DLP1, DRP1, DNM1L, Dynamin-1-like protein, Dnm1p/Vps1p-like protein, Dynamin family member proline-rich carboxyl-terminal domain less, Dynamin-like protein, Dynamin-like protein 4, Dynamin-like protein IV, Dynamin-related protein 1, DVLP, Dymple, HdynIV

Recommended products

Rabbit Recombinant Monoclonal DRP1 phospho S616 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27387-57
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The DRP1 protein also known as Dynamin-Related Protein 1 has a molecular weight of approximately 80 kDa. DRP1 is a GTPase that plays a central role in mitochondrial fission. The protein is widely expressed in many tissues with high levels in the brain heart and skeletal muscles. It functionally interacts with other proteins on the mitochondrial membrane to drive the division of mitochondria. As a mechanism DRP1 assembles into ring-like structures around constriction points on the outer mitochondrial membrane facilitating membrane scission.

Biological function summary

DRP1 regulates mitochondrial morphology by controlling mitochondrial division. This process is essential in maintaining the balance between mitochondrial fission and fusion which affects mitochondrial distribution and function. DRP1 also associates with protein complexes involved in this dynamic balance including MFF (mitochondrial fission factor) and FIS1 (fission 1 protein). Phosphorylation state of DRP1 influences its activity; different phosphorylation sites either activate or inhibit its function.

Pathways

The mechanical action of DRP1 integrates closely into the mitochondrial quality control and apoptosis pathways. Mitochondrial fission driven by DRP1 is necessary for the removal of damaged mitochondria through mitophagy. It also influences the apoptotic pathway where DRP1 translocates to mitochondria under pro-apoptotic signals often in interaction with proteins such as Bax and Bak that promote cytochrome c release. This relationship affects cell survival and energy homeostasis.

Associated diseases and disorders

Improper regulation of DRP1 is linked to neurodegenerative diseases like Alzheimer's disease and Parkinson's disease. Altered DRP1 activity or expression disrupts mitochondrial homeostasis contributing to neuronal cell death pathways. Connections with proteins such as Tau in Alzheimer's and Parkin in Parkinson's influence the progression of these disorders. Additionally DRP1's role in cardiac disorders highlights its importance in maintaining normal cardiac function through mitochondrial regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755), expandable thumbnail

    Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the lower MW band at approximately 20 kDa is unknown.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19436752).

    Anti-DRP1 antibody [EPR19274] ab184247 was used as the loading control at a 1/1000 dilution.

    All lanes: Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) at 1/1000 dilution

    Lane 1: Untreated HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (untreated membrane) at 20 µg

    Lane 2: HAP1 treated with 100 ng/ml nocodazole for 16 hours whole cell lysate (untreated membrane) at 20 µg

    Lane 3: Untreated HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (phosphatase treated membrane) at 20 µg

    Lane 4: HAP1 treated with 100 ng/ml nocodazole for 16 hours whole cell lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 82 kDa

    Observed band size: 83 kDa

    Exposure time: 92s

  • Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755), expandable thumbnail

    Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (Anti-DRP1 antibody [EPR19274] ab184247) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) at 1/1000 dilution

    Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg

    Lane 2: PC-12 whole cell lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 83 kDa

    Exposure time: 180s

  • Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755), expandable thumbnail

    Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    The identity of the lower MW band at approximately 20 kDa (in lane 2) is unknown. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (Anti-DRP1 antibody [EPR19274] ab184247) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 treated with /ml nocodazole for 17 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 83 kDa

    Exposure time: 180s

  • Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755), expandable thumbnail

    Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    Immunoprecipitation experiments were performed with an anti-6X His tag® antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) at 1:5000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (Anti-DRP1 antibody [EPR19274] ab184247) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) at 1/1000 dilution

    Lane 1: Untreated 293T cells transfected with a human DRP1 expression vector containi a myc-His-tag® lysate at 20 µg

    Lane 2: 293T cells transfected with a human DRP1 expression vector containing a myc-His-tag®. Overexpressi cells were treated with 100/ml nocodazole for 17 hours whole cell lysate at 20 µg

    Lane 3: Untreated 293T cells transfected with a human DRP1(S616A mutant ) expression vector containi a myc-His-tag® lysate at 20 µg

    Lane 4: 293T cells transfected with a human DRP1(S616A mutant) expression vector containing a myc-His-tag®. Overexpressi cells were treated with 100/ml nocodazole for 17 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 83 kDa

    Exposure time: 3s

  • Dot Blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755), expandable thumbnail

    Dot Blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755)

    Dot blot analysis of DRP1 (phospho S616) using ab314755 at 1:1000 (0.514 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
    Exposure time: 180 seconds.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

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