AB314755

Anti-DRP1 (phospho S616) antibody [EPR27387-57]

20ul selling size
RabMAb
Recombinant
What is this?

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(8 Publications)

Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) is a rabbit monoclonal antibody detecting DRP1 in Western Blot, Dot Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

DLP1, DRP1, DNM1L, Dynamin-1-like protein, Dnm1p/Vps1p-like protein, Dynamin family member proline-rich carboxyl-terminal domain less, Dynamin-like protein, Dynamin-like protein 4, Dynamin-like protein IV, Dynamin-related protein 1, DVLP, Dymple, HdynIV

5 Images

Supplier Data

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 20 kDa is unknown.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19436752).

ab184247 was used as the loading control at a 1/1000 dilution.

All lanes:

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) at 1/1000 dilution

Lane 1:

Untreated HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

HAP1 treated with 100 ng/ml nocodazole for 16 hours whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (phosphatase treated membrane) at 20 µg

Lane 4:

HAP1 treated with 100 ng/ml nocodazole for 16 hours whole cell lysate (phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 83 kDa

false

Exposure time: 92s

Supplier Data

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

PC-12 whole cell lysate (phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 83 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The identity of the lower MW band at approximately 20 kDa (in lane 2) is unknown. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 treated with /ml nocodazole for 17 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 83 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Immunoprecipitation experiments were performed with an anti-6X His tag® antibody (ab213204) at 1 : 5000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) at 1/1000 dilution

Lane 1:

Untreated 293T cells transfected with a human DRP1 expression vector containi a myc-His-tag® lysate at 20 µg

Lane 2:

293T cells transfected with a human DRP1 expression vector containing a myc-His-tag®. Overexpressi cells were treated with 100/ml nocodazole for 17 hours whole cell lysate at 20 µg

Lane 3:

Untreated 293T cells transfected with a human DRP1(S616A mutant ) expression vector containi a myc-His-tag® lysate at 20 µg

Lane 4:

293T cells transfected with a human DRP1(S616A mutant) expression vector containing a myc-His-tag®. Overexpressi cells were treated with 100/ml nocodazole for 17 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 83 kDa

false

Exposure time: 3s

Supplier Data

Dot Blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Dot blot analysis of DRP1 (phospho S616) using ab314755 at 1 : 1000 (0.514 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 180 seconds. Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Carrier free

Anti-DRP1 (phospho S616) antibody [EPR27387-57] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27387-57

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-DRP1 (phospho S616) antibody [EPR27387-57] (ab314755) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of DRP1?
Anti-DRP1 (phospho S616) [EPR27387-57] (ab314755) specifically detects a band for DRP1 (UniProt: O00429) at a molecular weight of 82kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR27387-57] also available for your convenience: ab314755, Carrier free - ab314756

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DRP1 protein also known as Dynamin-Related Protein 1 has a molecular weight of approximately 80 kDa. DRP1 is a GTPase that plays a central role in mitochondrial fission. The protein is widely expressed in many tissues with high levels in the brain heart and skeletal muscles. It functionally interacts with other proteins on the mitochondrial membrane to drive the division of mitochondria. As a mechanism DRP1 assembles into ring-like structures around constriction points on the outer mitochondrial membrane facilitating membrane scission.

Biological function summary

DRP1 regulates mitochondrial morphology by controlling mitochondrial division. This process is essential in maintaining the balance between mitochondrial fission and fusion which affects mitochondrial distribution and function. DRP1 also associates with protein complexes involved in this dynamic balance including MFF (mitochondrial fission factor) and FIS1 (fission 1 protein). Phosphorylation state of DRP1 influences its activity; different phosphorylation sites either activate or inhibit its function.

Pathways

The mechanical action of DRP1 integrates closely into the mitochondrial quality control and apoptosis pathways. Mitochondrial fission driven by DRP1 is necessary for the removal of damaged mitochondria through mitophagy. It also influences the apoptotic pathway where DRP1 translocates to mitochondria under pro-apoptotic signals often in interaction with proteins such as Bax and Bak that promote cytochrome c release. This relationship affects cell survival and energy homeostasis.

Improper regulation of DRP1 is linked to neurodegenerative diseases like Alzheimer's disease and Parkinson's disease. Altered DRP1 activity or expression disrupts mitochondrial homeostasis contributing to neuronal cell death pathways. Connections with proteins such as Tau in Alzheimer's and Parkin in Parkinson's influence the progression of these disorders. Additionally DRP1's role in cardiac disorders highlights its importance in maintaining normal cardiac function through mitochondrial regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in mitochondrial and peroxisomal division (PubMed : 11514614, PubMed : 12499366, PubMed : 17301055, PubMed : 17460227, PubMed : 17553808, PubMed : 18695047, PubMed : 18838687, PubMed : 19342591, PubMed : 19411255, PubMed : 19638400, PubMed : 23283981, PubMed : 23530241, PubMed : 23921378, PubMed : 26992161, PubMed : 27145208, PubMed : 27145933, PubMed : 27301544, PubMed : 27328748, PubMed : 29478834, PubMed : 32439975, PubMed : 32484300, PubMed : 9570752, PubMed : 9786947). Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism (PubMed : 23530241, PubMed : 23584531, PubMed : 33850055). The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes (PubMed : 23283981, PubMed : 23921378, PubMed : 29899447). While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane (PubMed : 29899447). Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner (PubMed : 32484300). Plays an important role in mitochondrial fission during mitosis (PubMed : 19411255, PubMed : 26992161, PubMed : 27301544, PubMed : 27328748). Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage (By similarity). Required for normal brain development, including that of cerebellum (PubMed : 17460227, PubMed : 26992161, PubMed : 27145208, PubMed : 27301544, PubMed : 27328748). Facilitates developmentally regulated apoptosis during neural tube formation (By similarity). Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues (By similarity). Required for formation of endocytic vesicles (PubMed : 20688057, PubMed : 23792689, PubMed : 9570752). Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles (PubMed : 17015472, PubMed : 23792689). Required for programmed necrosis execution (PubMed : 22265414). Rhythmic control of its activity following phosphorylation at Ser-637 is essential for the circadian control of mitochondrial ATP production (PubMed : 29478834).. Isoform 1. Inhibits peroxisomal division when overexpressed.. Isoform 4. Inhibits peroxisomal division when overexpressed.

See full target information DNM1L phospho S616

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed41059571

2025

AI662270/GRP94 axis couples the unfolded protein response to mitochondrial dynamics during acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Suling Ding,Wen Liu,Zhiwei Zhang,Xiyang Yang,Dili Sun,Jianfu Zhu,Xiaowei Zhu,Shijun Wang,Mengshi Xie,Hongyu Shi,Junbo Ge,Xiangdong Yang

American journal of cancer research 15:3693-3711 PubMed40948526

2025

Aurora-A inhibits hepatocellular carcinoma cell ferroptosis to mediate immune escape by disrupting phosphatidylethanolamine biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Fan,Yiqian Liu,Yucheng Cai,Xinnan Sun,Jiaxuan Li,Yiyang Xu,Changchun Sun,Shiyun Cui

Cardiovascular diabetology 24:331 PubMed40804395

2025

Mitochondrial ultrastructural pathology in diabetic cardiomyopathy: integrated analysis via scanning electron microscopy and 3D visualization imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wang,Linghao Dai,Haowei Liang,Jiayu He,Jiayi Zhou,Yang Guan,Hui Wang

Mediators of inflammation 2025:3675276 PubMed40196168

2025

Mdivi-1 Attenuates Sepsis-Associated Acute Lung Injury by Inhibiting M1 Alveolar Macrophage Polarization and Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zhang,Hui Fan,Li Su,Yanni Wang,Guozhong Chen

The Journal of biological chemistry 301:108111 PubMed39706273

2024

PRDX4 mitigates diabetic retinopathy by inhibiting reactive gliosis, apoptosis, ER stress, oxidative stress, and mitochondrial dysfunction in Müller cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Huang,Yuting Zhang,Yuan Liu,Yinan Jin,Hongwei Yang

Drug development research 85:e70033 PubMed39660541

2024

Heme Oxygenase-1 Overexpression Activates the IRF1/DRP1 Signaling Pathway to Promote M2-Type Polarization of Spinal Cord Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Wenping Lin,Ziming Cai,Jinzhu Liang,Ping Miao,Ye Ruan,Pian Li,Shuhui Lin,He Tian,Qinghe Yu,Xu He

Journal of ovarian research 17:188 PubMed39289738

2024

Mechanism study of YangJing ZhongYu decoction on regulating mitochondrial dynamics of ovarian granular cells and improving diminished ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Jilin Kuang

International journal of molecular sciences 25: PubMed38674016

2024

The CaMK Family Differentially Promotes Necroptosis and Mouse Cardiac Graft Injury and Rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Lu,Jifu Jiang,Jeffery Min,Xuyan Huang,Patrick McLeod,Weihua Liu,Aaron Haig,Lakshman Gunaratnam,Anthony M Jevnikar,Zhu-Xu Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DRP1 (phospho S616) antibody [EPR27387-57]

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Dot Blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (AB314755)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise