Anti-DRP1 (phospho S616) antibody [EPR27387-57] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] - BSA and Azide free (AB314756)

This data was developed using ab314755, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 20 kDa is unknown.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19436752).

ab184247 was used as the loading control at a 1/1000 dilution.

All lanes:

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (<a href='/en-us/products/primary-antibodies/drp1-phospho-s616-antibody-epr27387-57-ab314755'>ab314755</a>) at 1/1000 dilution

Lane 1:

Untreated HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

HAP1 treated with 100 ng/ml nocodazole for 16 hours whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (phosphatase treated membrane) at 20 µg

Lane 4:

HAP1 treated with 100 ng/ml nocodazole for 16 hours whole cell lysate (phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 83 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] - BSA and Azide free (AB314756)

This data was developed using ab314755, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (<a href='/en-us/products/primary-antibodies/drp1-phospho-s616-antibody-epr27387-57-ab314755'>ab314755</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

PC-12 whole cell lysate (phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 83 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] - BSA and Azide free (AB314756)

This data was developed using ab314755, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The identity of the lower MW band at approximately 20 kDa (in lane 2) is unknown. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (<a href='/en-us/products/primary-antibodies/drp1-phospho-s616-antibody-epr27387-57-ab314755'>ab314755</a>) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 treated with /ml nocodazole for 17 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 83 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] - BSA and Azide free (AB314756)

This data was developed using ab314755, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Immunoprecipitation experiments were performed with an anti-6X His tag® antibody (ab213204) at 1 : 5000 dilution. In western blot, anti-DRP1 antibody [EPR19274] - Total protein Control (ab184247) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] (<a href='/en-us/products/primary-antibodies/drp1-phospho-s616-antibody-epr27387-57-ab314755'>ab314755</a>) at 1/1000 dilution

Lane 1:

Untreated 293T cells transfected with a human DRP1 expression vector containi a myc-His-tag® lysate at 20 µg

Lane 2:

293T cells transfected with a human DRP1 expression vector containing a myc-His-tag®. Overexpressi cells were treated with 100/ml nocodazole for 17 hours whole cell lysate at 20 µg

Lane 3:

Untreated 293T cells transfected with a human DRP1(S616A mutant ) expression vector containi a myc-His-tag® lysate at 20 µg

Lane 4:

293T cells transfected with a human DRP1(S616A mutant) expression vector containing a myc-His-tag®. Overexpressi cells were treated with 100/ml nocodazole for 17 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 83 kDa

false

Exposure time: 3s

• Dot

Supplier Data

Dot Blot - Anti-DRP1 (phospho S616) antibody [EPR27387-57] - BSA and Azide free (AB314756)

This data was developed using ab314755, the same antibody clone in a different buffer formulation. Dot blot analysis of DRP1 (phospho S616) using ab314755 at 1 : 1000 (0.514 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 180 seconds. Blocking and diluting buffer and concentration : 5% NFDM/TBST.