Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free
- BOND RX™ Validated
- What is this?
4
(10 Reviews)
|
(141 Publications)
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting ds DNA in IHC-P.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 140 publications
- Trusted since 2005
View Alternative Names
Double stranded DNA, Double stranded deoxyribonucleic acid, dsDNA
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (AB27156)](https://content.abcam.com/products/images/ds-dna-antibody-35i9-dna-bsa-and-azide-free-ab27156--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102861.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (AB27156)
IHC image of ds DNA staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab27156, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Reactivity data
Product details
Product Specifications
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156) is a mouse monoclonal antibody provided in a carrier-free, conjugation ready format.
ab27156 is validated in IHC-Pin human samples.
Carrier-free antibodies are compatible with our best-in-class range of Lightning-Link® conjugation kits.
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156) specifically detects ds DNA and is sold in 100 µL selling sizes.
Quality and Validation
Antibody clone 35I9 DNA has been cited over 141 times in peer reviewed journals and is trusted by the scientific community.
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156) has 10 independent reviews from customers.
This antibody has been shown to be useful, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength.
Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113). Or search our wide range of secondary antibodies for use with your experiment.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (141)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in immunology 14:1235936 PubMed38152396
2023
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Science advances 9:eadi6681 PubMed38055811
2023
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Cell death & disease 14:724 PubMed37935658
2023
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Pharmaceuticals (Basel, Switzerland) 16: PubMed38004392
2023
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The EMBO journal 42:e113104 PubMed37855233
2023
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Open biology 13:230113 PubMed37788708
2023
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Materials today. Bio 23:100809 PubMed37779919
2023
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Nature genetics 55:1721-1734 PubMed37735199
2023
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Methods in molecular biology (Clifton, N.J.) 2701:135-148 PubMed37574479
2023
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Methods in molecular biology (Clifton, N.J.) 2701:149-156 PubMed37574480
2023
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Product promise
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