AB27156

Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free

Name: Anti-ds DNA antibody [35I9 DNA], BSA and Azide free (ab27156) | Abcam
Brand: Abcam Limited
SKU: AB27156
Price: 390 USD
Availability: InStock
Rating: 4 (10 reviews)

BOND RX™ Validated
What is this?

(10 Reviews)

(226 Publications)

Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting ds DNA in IHC-P.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 140 publications
- Trusted since 2005

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (AB27156)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (AB27156)

IHC image of ds DNA staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab27156, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

35I9 DNA

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Applications

IHC-P

applications

Specificity

Primarily Double stranded DNA. Measurements by immuno-CE yielded K_D's of 0.71 µM and 0.09 µM, for the interaction of this antibody with ss- and dsDNA, respectively. Strong reactivity with both ss- and dsDNA has been observed on dotblots as well as very weak reactivity with RNA.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Nucleic Acid": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.05 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Product details

Product Specifications
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156) is a mouse monoclonal antibody provided in a carrier-free, conjugation ready format.
ab27156 is validated in IHC-Pin human samples.
Carrier-free antibodies are compatible with our best-in-class range of Lightning-Link® conjugation kits.
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156) specifically detects ds DNA and is sold in 100 µL selling sizes.

Quality and Validation
Antibody clone 35I9 DNA has been cited over 141 times in peer reviewed journals and is trusted by the scientific community.
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156) has 10 independent reviews from customers.

This antibody has been shown to be useful, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength.
Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113). Or search our wide range of secondary antibodies for use with your experiment.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information ds DNA

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Publications (226)

Recent publications for all applications. Explore the full list and refine your search

Biology methods & protocols 10:bpaf068 PubMed41018846

2025

A robust protocol for proteomic profiling of secreted proteins in conditioned culture medium.

Applications

Unspecified application

Species

Unspecified reactive species

Takayoshi Otsuka,Atsushi Hatano,Masaki Matsumoto,Hideaki Matsui

Nucleic acids research 53: PubMed40842236

2025

Hemin-binding DNA structures on the surface of bacteria promote extracellular electron transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Obinna M Ajunwa,Gabriel Antonio S Minero,Sissel D Jensen,Rikke L Meyer

Nature communications 16:7458 PubMed40796804

2025

Epigenetic control of topoisomerase 1 activity presents a cancer vulnerability.

Applications

Unspecified application

Species

Unspecified reactive species

Tae-Hee Lee,Colina X Qiao,Vladislav Kuzin,Yuepeng Shi,Marina Farkas,Zhiyan Zhao,Vijayalalitha Ramanarayanan,Tongyu Wu,Tianyi Guan,Xianzhen Zhou,David Corujo,Marcus Buschbeck,Laura Baranello,Philipp Oberdoerffer

Cell death & disease 16:540 PubMed40691137

2025

Molecular features of TNBC govern heterogeneity in the response to radiation and autophagy inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Fischer,Maximilian Schmid,Anna Ohradanova-Repic,Rebecca Schneeweiss,Jana Hadatsch,Odysseus Grünert,Johannes Benedum,Anna Röhrer,Felix Staudinger,Philipp Schatzlmaier,Niccolo Bragato,Sandra Barna,Magdalena Engl,Ava Kleinwächter,Dietmar Georg,Joachim Widder,Sylvia Kerschbaum-Gruber,Dea Slade

Nucleic acids research 53: PubMed40685547

2025

The dual ubiquitin binding mode of SPRTN secures rapid spatiotemporal proteolysis of DNA-protein crosslinks.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Song,Yichen Zhao,Annamaria Ruggiano,Christina Redfield,Joseph A Newman,Xiaosheng Zhu,Marta García-Flores,Abimael Cruz-Migoni,Rebecca Roddan,Anna Pérez-Ràfols,Peter J McHugh,Paul R Elliott,Kristijan Ramadan

Nature communications 16:6391 PubMed40640125

2025

Super-resolution microscopy of mitochondrial mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Stoldt,Frederike Maass,Michael Weber,Sven Dennerlein,Peter Ilgen,Jutta Gärtner,Aysenur Canfes,Sarah V Schweighofer,Daniel C Jans,Peter Rehling,Stefan Jakobs

Oncogene 44:3255-3271 PubMed40640336

2025

Human RNase H2 upregulation counteracts oncogene- and chemotherapy-induced replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Rosanna J Wilkins,Abirami Kannan,Siobhan A Plass,Claire Wilson,Richard D W Kelly,Claire H M Tang,Panagiotis Kotsantis,Martin A M Reijns,Aditi Kanhere,Eva Petermann

Journal of neuroinflammation 22:176 PubMed40634978

2025

Pharmacological inhibition of the cGAS-STING pathway suppresses microglia pyroptosis in sepsis-associated encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Quan Zeng,Yang Qi,Hui Yu,Ying Xu,Jin-Xing Chen,You-Wei Zheng,Gui-Fei Zhang,Qiao-Ling Zhang,Yan-Hua Zheng,Jing Guo,Zi-Hong Zhao,Fa-Sheng Wang,Gui-Lin Jin

Scientific reports 15:23191 PubMed40603988

2025

Ionizing radiation triggers the release of mitochondrial DNA into the cytosol as a signal of mitochondrial damage.

Applications

Unspecified application

Species

Unspecified reactive species

Tsutomu Shimura,Yui Takahashi,Chihiro Saito,Riko Maida,Megumi Sasatani,Tatsuki Kunoh,Akira Ushiyama

Nucleic acids research 53: PubMed40598896

2025

Loss of DHX36/G4R1, a G4 resolvase, drives genome instability and regulates innate immune gene expression in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anna R Bartosik,Pei-Chi Hou,Shangming Tang,James P Vaughn,Philip J Smaldino,Aakrosh Ratan,Marty W Mayo,Yuh-Hwa Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (AB27156)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (226)

Product promise