Mouse Recombinant Monoclonal ds DNA antibody. Carrier free. Suitable for IHC-P and reacts with Nucleic Acid samples. Immunogen corresponding to Cell preparation containing ds DNA protein.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (AB273144), expandable thumbnail](https://content.abcam.com/products/images/ds-dna-antibody-rdsd-4565-bsa-and-azide-free-ab273144--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103188.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (AB273144)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (AB273144), expandable thumbnail](https://content.abcam.com/products/images/ds-dna-antibody-rdsd-4565-bsa-and-azide-free-ab273144--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img2827.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (AB273144)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing ds DNA protein. The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Nucleic Acid | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Nucleic Acid | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Alternative names
Double stranded DNA, Double stranded deoxyribonucleic acid, dsDNA
Recommended products
Mouse Recombinant Monoclonal ds DNA antibody. Carrier free. Suitable for IHC-P and reacts with Nucleic Acid samples. Immunogen corresponding to Cell preparation containing ds DNA protein.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing ds DNA protein. The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- rDSD/4565
- Light chain type
- kappa
- Concentration
- Purification notes
Ab purified from Bioreactor Concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab273144 is the carrier-free version of Anti-ds DNA antibody [rDSD/4565] ab273137.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (ab273144), expandable thumbnail](https://content.abcam.com/products/images/ds-dna-antibody-rdsd-4565-bsa-and-azide-free-ab273144--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103188.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (ab273144)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (ab273144)
Paraffin embedded human lymph node tissue stained for double stranded DNA (dsDNA) using Anti-ds DNA antibody [rDSD/4565] ab273137 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ds DNA antibody [rDSD/4565] ab273137).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (ab273144), expandable thumbnail](https://content.abcam.com/products/images/ds-dna-antibody-rdsd-4565-bsa-and-azide-free-ab273144--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img2827.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (ab273144)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] - BSA and Azide free (ab273144)
Paraffin embedded human colon tissue stained for double stranded DNA (dsDNA) using Anti-ds DNA antibody [rDSD/4565] ab273137 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ds DNA antibody [rDSD/4565] ab273137).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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