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AB270327

Anti-Dscam antibody [EPR22435-247] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal DSCAM antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat, Human samples.

View Alternative Names

Cell adhesion molecule DSCAM, CHD2, Down syndrome cell adhesion molecule, DSCAM

1 Images
Sandwich ELISA - Anti-Dscam antibody [EPR22435-247] - BSA and Azide free (Detector) (AB270327)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Dscam antibody [EPR22435-247] - BSA and Azide free (Detector) (AB270327)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab269543).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22435-247

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab270327 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify DSCAM. The recommended pair for sandwich ELISA is:
Capture: ab270326, DSCAM Capture Antibody (unconjugated)
Detector: ab270327, DSCAM Detector Antibody (unconjugated)
The reference range value is 203.125 - 13000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dscam known as Down Syndrome Cell Adhesion Molecule is a protein with a mass of around 230 kDa. It functions as a cell adhesion molecule and is involved in axon guidance. Dscam is expressed in various tissues with high levels in the nervous system particularly during developmental stages. The protein plays a role in neural development by ensuring proper neuronal connections.
Biological function summary

Dscam contributes to neuronal differentiation and synaptic targeting. It belongs to the immunoglobulin superfamily and is involved in forming non-covalent complexes with other cell adhesion molecules. The protein facilitates homophilic binding which regulates axonal and dendritic branching. This process ensures neural circuits form correctly impacting brain development and function.

Pathways

Dscam participates in the neural development pathway which includes interactions with the Robo receptor affecting axonal migration and guidance. The protein also links to the slit-Robo pathway playing a substantial role in neural patterning. These interactions ensure the precise wiring required for functional neural networks influencing how neural signals propagate.

Dscam's dysregulation connects to neurological conditions like Down syndrome and autism spectrum disorders. It interacts with proteins involved in these disorders like the ROBO proteins which mediate axon guidance. Accumulating evidence suggests that altered Dscam expression impairs neuronal connectivity contributing to the pathophysiology of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies (PubMed : 10925149). Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. Might also collaborate with UNC5C in NTN1-mediated axon repulsion independently of DCC (By similarity). In spinal cord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding (PubMed : 18585357, PubMed : 19196994). Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38 (PubMed : 18585357, PubMed : 19196994). Adhesion molecule that promotes lamina-specific synaptic connections in the retina : expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions (By similarity).
See full target information Down syndrome cell adhesion molecule
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Product promise

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