AB216892

Anti-DSPP antibody

Name: Anti-DSPP antibody (ab216892) | Abcam
Brand: Abcam Limited
SKU: AB216892

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(20 Publications)

Rabbit Polyclonal DSPP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 20 publications.

View Alternative Names

Dentin sialophosphoprotein, DSPP

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSPP antibody (AB216892)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSPP antibody (AB216892)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human laryngocarcinoma tissue labeling DSPP with ab216892 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DSPP also known as Dentin Sialophosphoprotein is a protein important for tooth development and dentin mineralization. It has a molecular mass of about 110-130 kDa which can vary due to post-translational modifications and cleavage into its components. This protein mainly expresses in odontoblasts the cells responsible for dentin formation but is also found in other tissues like bone and inner ear. The protein shares structural similarities with other members of the SIBLING family which are known to interact with mineralized tissues.

Biological function summary

DSPP plays an essential role in the formation and maintenance of dentin structure. It first undergoes proteolytic cleavage into two main products: Dentin Sialoprotein (DSP) and Dentin Phosphoprotein (DPP). These components integrate into the extracellular matrix where they contribute to the mineralization process of dentin. DSPP does not act alone but participates in a protein complex involving other matrix molecules that help regulate mineralization.

Pathways

DSPP is a component of key mineralization pathways which ensure proper formation and hardening of dentin. One significant pathway is the Transforming Growth Factor-beta (TGF-beta) signaling pathway which influences dental tissue response during tooth development. Within this pathway DSPP interacts with proteins like Bone Morphogenetic Protein (BMP) to facilitate signaling processes required for odontoblast differentiation and function.

Mutations or dysfunctional expression of DSPP link to dental defects such as Dentinogenesis Imperfecta and dentin dysplasia. These conditions lead to abnormal dentin formation causing teeth to be discolored weak or misformed. Additionally DSPP shows interactions with proteins associated with bone disorders which suggests a role in conditions like osteogenesis imperfecta. Understanding these associations can guide therapeutic approaches in managing dental and skeletal abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.

See full target information DSPP

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:179 PubMed40234972

2025

KLF6-mediated glutamine metabolism governs odontogenic differentiation and matrix mineralization of dental pulp stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhi Wu,Zekai Xu,Yulian Zhang,Xiatong Zhang,Xiaoyuan Huang,Zhijian Xie,Zhuo Chen

Journal of functional biomaterials 16: PubMed40137373

2025

Effects of ECM Components on Periodontal Ligament Stem Cell Differentiation Under Conditions of Disruption of Wnt and TGF-β Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Alla V Kuznetsova,Olga P Popova,Tamara I Danilova,Andrey V Latyshev,Oleg O Yanushevich,Alexey A Ivanov

Journal of dentistry (Shiraz, Iran) 26:55-60 PubMed40092544

2025

Dentin Sialophosphoprotein Expression Profile in Developing Human Primary Teeth: An Experimental Study.

Applications

Unspecified application

Species

Unspecified reactive species

Soussan Irani,Shohreh Alimohammadi,Fatemeh Ataei

Cells 12: PubMed37830549

2023

Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey A Ivanov,Alla V Kuznetsova,Olga P Popova,Tamara I Danilova,Andrey V Latyshev,Oleg O Yanushevich

Oral diseases 29:2827-2836 PubMed36579641

2023

KDM4D enhances osteo/dentinogenic differentiation and migration of SCAPs via binding to RPS5.

Applications

Unspecified application

Species

Unspecified reactive species

Hanbing Liang,Qian Li,Ning Wang,Chunxiong Wang,Shaojing Shi,Haoqing Yang,Yangyang Cao,Ruitang Shi,Luyuan Jin,Chen Zhang

Biomolecules 13: PubMed36671506

2023

Decellularized Matrix Induced Spontaneous Odontogenic and Osteogenic Differentiation in Periodontal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey A Ivanov,Tamara I Danilova,Alla V Kuznetsova,Olga P Popova,Oleg O Yanushevich

International dental journal 73:362-369 PubMed35999071

2022

MiR-335-3p/miR-155-5p Involved in IGFBP7-AS1-Enhanced Odontogenic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ningxin Zhu,Dan Wang,Fei Xie,Man Qin,Yuanyuan Wang

Stem cells international 2022:2236250 PubMed35530415

2022

Enamel Matrix Derivative Enhances the Odontoblastic Differentiation of Dental Pulp Stem Cells via Activating MAPK Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Beidi Zhang,Min Xiao,Xiaogang Cheng,Yu Bai,Hanze Chen,Qing Yu,Lihong Qiu

Molecular therapy. Nucleic acids 25:116-126 PubMed34401209

2021

lncRNA H19 facilitates the proliferation and differentiation of human dental pulp stem cells via EZH2-dependent LATS1 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Du,Xiaoming Shi,Aizhong Guan

Clinical oral investigations 25:6321-6333 PubMed33822289

2021

Tissue-specific biomarkers in gingival crevicular fluid are correlated with external root resorption caused by constant mechanical load: an in vivo study.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Yue Huang,Sung-Hwan Choi,Hwi-Dong Jung,Hyun Sil Kim,Chung-Ju Hwang,Kee-Joon Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DSPP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSPP antibody (AB216892)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise