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Rabbit Recombinant Monoclonal DSTN antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-DSTN antibody [EPR15828] - BSA and Azide free (AB251129), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15828] - BSA and Azide free (AB251129), expandable thumbnail
  • Immunoprecipitation - Anti-DSTN antibody [EPR15828] - BSA and Azide free (AB251129), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15828] - BSA and Azide free (AB251129), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.

Alternative names

ACTDP, DSN, DSTN, Destrin, Actin-depolymerizing factor, ADF

Recommended products

Rabbit Recombinant Monoclonal DSTN antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15828
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251129 is the carrier-free version of Anti-DSTN antibody [EPR15828] ab192262.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DSTN also known as destrin or actin depolymerizing factor is an actin-binding protein with a relatively low molecular mass of approximately 19 kDa. DSTN plays a significant role in actin filament dynamics by enhancing the disassembly of actin filaments into monomeric actin components thereby regulating cytoskeleton organization. The protein is present in a wide range of tissues including the brain and skeletal muscles indicating its importance in various physiological processes.

Biological function summary

DSTN regulates actin cytoskeleton remodeling impacting cell shape and motility. It acts by binding to actin enhancing the turnover and dismantling of actin filaments which is critical for maintaining cellular architecture. DSTN is not known to be part of a larger protein complex but it interacts with other actin-related proteins to exert its function efficiently. Its role in cell movement and structure integrates it into larger cellular processes such as cell division migration and potentially signal transduction.

Pathways

Cytoskeletal rearrangement and cell motility highlight the involvement of DSTN. DSTN associates closely with the Rho and Rac signaling pathways pivotal for cytoskeletal changes and cell division processes. Through these pathways DSTN contributes to modulating cellular responses by interacting with proteins like cofilin another actin-binding protein. This interaction with signaling pathways highlights DSTN's broader influence on cellular functions.

Associated diseases and disorders

DSTN has an association with neurodegenerative diseases and cancer progression. Aberrations in its activity can result in cytoskeletal dysfunctions to which disorders such as Alzheimer’s disease relate due to the importance of cytoskeletal integrity in neuron function. Additionally DSTN's involvement in cell motility links it to cancer metastasis where unchecked cell movement promotes tumor spread. In these diseases DSTN collaborates mutably with the tau protein and other cytoskeletal regulators highlighting DSTN's relevance in pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129), expandable thumbnail

    Western blot - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)

    This data was developed using Anti-DSTN antibody [EPR15828] ab192262, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-DSTN antibody [EPR15828] (Anti-DSTN antibody [EPR15828] ab192262) at 1/5000 dilution

    Lane 1: MCF-7 cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)

    This data was developed using Anti-DSTN antibody [EPR15828] ab192262, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% formaldehyde fixed MCF-7 cells labeling DSTN using Anti-DSTN antibody [EPR15828] ab192262 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. The two negative controls; 1. Primary ab concentration (anti-DSTN) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-DSTN) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.

  • Immunoprecipitation - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129), expandable thumbnail

    Immunoprecipitation - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)

    This data was developed using Anti-DSTN antibody [EPR15828] ab192262, the same antibody clone in a different buffer formulation.Lysate from A549 cells was immunoprecipitated with Anti-DSTN antibody [EPR15828] ab192262 at a 1/30 dilution. For the subsequent blot, Anti-DSTN antibody [EPR15828] ab192262 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-DSTN antibody [EPR15828] (Anti-DSTN antibody [EPR15828] ab192262)

    Predicted band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)

    This data was developed using Anti-DSTN antibody [EPR15828] ab192262, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human cervical carcinoma tissue sections labeling DSTN using Anti-DSTN antibody [EPR15828] ab192262 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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