Anti-DSTN antibody [EPR15828] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15828] - BSA and Azide free (AB251129)

This data was developed using ab192262, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% formaldehyde fixed MCF-7 cells labeling DSTN using ab192262 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. The two negative controls; 1. Primary ab concentration (anti-DSTN) is 1 : 50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1 : 500 dilution; 2. Primary ab concentration (anti-DSTN) is 1 : 50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1 : 500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15828] - BSA and Azide free (AB251129)

This data was developed using ab192262, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded human cervical carcinoma tissue sections labeling DSTN using ab192262 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-DSTN antibody [EPR15828] - BSA and Azide free (AB251129)

This data was developed using ab192262, the same antibody clone in a different buffer formulation.

Lysate from A549 cells was immunoprecipitated with ab192262 at a 1/30 dilution. For the subsequent blot, ab192262 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DSTN antibody [EPR15828] (<a href='/en-us/products/primary-antibodies/dstn-antibody-epr15828-ab192262'>ab192262</a>)

Predicted band size: 19 kDa

false

• WB

Supplier Data

Western blot - Anti-DSTN antibody [EPR15828] - BSA and Azide free (AB251129)

This data was developed using ab192262, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DSTN antibody [EPR15828] (<a href='/en-us/products/primary-antibodies/dstn-antibody-epr15828-ab192262'>ab192262</a>) at 1/5000 dilution

Lane 1:

MCF-7 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

false