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AB70621

Anti-DTX3L antibody

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(2 Publications )

Rabbit Polyclonal DTX3L antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DTX3L aa 700 to C-terminus.
2 Images
Immunoprecipitation - Anti-DTX3L antibody (AB70621)
  • IP

Unknown

Immunoprecipitation - Anti-DTX3L antibody (AB70621)

Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70621 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab70621 at 1 µg/ml.

All lanes:

Immunoprecipitation - Anti-DTX3L antibody (ab70621)

Predicted band size: 84 kDa

false

Western blot - Anti-DTX3L antibody (AB70621)
  • WB

Unknown

Western blot - Anti-DTX3L antibody (AB70621)

All lanes:

Western blot - Anti-DTX3L antibody (ab70621) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 84 kDa

Observed band size: 35 kDa,40 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DTX3L aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TDB6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses (PubMed : 12670957, PubMed : 19818714, PubMed : 23230272, PubMed : 26479788). Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4 (PubMed : 28525742). In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1) (PubMed : 19818714). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me) (PubMed : 19818714). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed : 23230272). By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication (PubMed : 26479788). Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed : 24790097). In addition, required for the recruitment of HGS and STAM to early endosomes (PubMed : 24790097). In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteasomal-mediated degradation (PubMed : 26479788).
See full target information DTX3L

Alternative Names

BBAP, DTX3L, E3 ubiquitin-protein ligase DTX3L, B-lymphoma- and BAL-associated protein, Protein deltex-3-like, RING-type E3 ubiquitin transferase DTX3L, Rhysin-2, Rhysin2
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Nature communications 16:5912 PubMed40595467

2025

Viruses hijack FPN1 to disrupt iron withholding and suppress host defense.

Applications

Unspecified application

Species

Unspecified reactive species

Li Tong,Jie Wang,Yunjin Ma,Chunying Wang,Yue Fu,Qi Li,Chengjiang Gao,Hui Song,Ying Qin,Chunyuan Zhao,Wei Zhao

Heliyon 8:e09476 PubMed35647352

2022

Short cytoplasmic isoform of IL1R1/CD121a mediates IL1β induced proliferation of synovium-derived mesenchymal stem/stromal cells through ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guo Tang,Yoshinori Asou,Etsuko Matsumura,Yusuke Nakagawa,Kazumasa Miyatake,Hiroki Katagiri,Tomomasa Nakamura,Hideyuki Koga,Keiichiro Komori,Ichiro Sekiya,Yoich Ezura,Kunikazu Tsuji
View all publications
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Product promise

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