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AB154867

Anti-DTYMK antibody [EPR10163]

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(5 Publications)

Rabbit Recombinant Monoclonal DTYMK antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CDC8, TMPK, TYMK, DTYMK, Thymidylate kinase, dTMP kinase

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] (AB154867)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] (AB154867)

Immunofluorescent analysis of HeLa cells labeling DTYMK with ab154867 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867)

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154867 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] (AB154867)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] (AB154867)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DTYMK with purified ab154867 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867)

Immunohistochemical analysis of paraffin embedded Human prostate hyperplasia tissue using ab154867 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154867 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling DTYMK with ab137875 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-DTYMK antibody [EPR10163] (AB154867)
  • WB

Unknown

Western blot - Anti-DTYMK antibody [EPR10163] (AB154867)

All lanes:

Western blot - Anti-DTYMK antibody [EPR10163] (ab154867) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10163

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), the immediate precursor for the DNA building block dTTP, with ATP as the preferred phosphoryl donor in the presence of Mg(2+).
See full target information DTYMK
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2404841121 PubMed39348545

2024

TCA metabolism regulates DNA hypermethylation in LPS and -induced immune tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Abhimanyu,Santiago Carrero Longlax,Tomoki Nishiguchi,Malik Ladki,Daanish Sheikh,Amera L Martinez,Emily M Mace,Sandra L Grimm,Thaleia Caldwell,Alexandra Portillo Varela,Rajagopal V Sekhar,Anna M Mandalakas,Mandla Mlotshwa,Sibuse Ginidza,Jeffrey D Cirillo,Robert S Wallis,Mihai G Netea,Reinout van Crevel,Cristian Coarfa,Andrew R DiNardo

BMC cancer 23:611 PubMed37400785

2023

Upregulation of hsa_circ_0002003 promotes hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Lisha Zhou,Qianwen Wang,Jun Hou,Xiangwei Wu,Lianghai Wang,Xueling Chen

World journal of oncology 14:84-93 PubMed36895992

2023

The Impact of DTYMK as a Prognostic Marker in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulaziz A Aloliqi,Abdul-Fattah Fararjeh,Ali Al-Khader,Ezidin Kaddumi,Alaa Abdulaziz Eisa,Weam Jaradat

Frontiers in oncology 11:625566 PubMed35004265

2021

MiR-148b-3p Regulates the Expression of DTYMK to Drive Hepatocellular Carcinoma Cell Proliferation and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Guifang He,Jing Qiu,Changchang Liu,Ben Tian,Duo Cai,Shihai Liu

Cell cycle (Georgetown, Tex.) 20:1681-1691 PubMed34369850

2021

DTYMK promote hepatocellular carcinoma proliferation by regulating cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhao Zhou,Rui Qin,Susu Shi,Hua Zhang,Chuanling Niu,Gaoda Ju,Sen Miao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com