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Rabbit Recombinant Monoclonal DTYMK antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-DTYMK antibody [EPR10163] (AB154867), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] (AB154867), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] (AB154867), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (AB154867), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Target data

Function

Catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), the immediate precursor for the DNA building block dTTP, with ATP as the preferred phosphoryl donor in the presence of Mg(2+).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal DTYMK antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10163

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-DTYMK antibody [EPR10163] (ab154867), expandable thumbnail

    Western blot - Anti-DTYMK antibody [EPR10163] (ab154867)

    All lanes: Western blot - Anti-DTYMK antibody [EPR10163] (ab154867) at 1/1000 dilution

    Lane 1: Raji cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (ab154867), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (ab154867)

    Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling DTYMK with ab137875 at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] (ab154867), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] (ab154867)

    Immunofluorescent analysis of HeLa cells labeling DTYMK with ab154867 at 1/100.

  • Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] (ab154867), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] (ab154867)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DTYMK with purified ab154867 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (ab154867), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (ab154867)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154867 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (ab154867), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (ab154867)

    Immunohistochemical analysis of paraffin embedded Human prostate hyperplasia tissue using ab154867 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (ab154867), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] (ab154867)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154867 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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