Rabbit Recombinant Monoclonal DTYMK antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--western-blot-img105675.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126787.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166)")
![Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunocytochemistry-immunofluorescence-img117455.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166)")
![Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--flow-cytometry-intracellular-img175933.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119630.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (AB249166)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), the immediate precursor for the DNA building block dTTP, with ATP as the preferred phosphoryl donor in the presence of Mg(2+).
Alternative names
CDC8, TMPK, TYMK, DTYMK, Thymidylate kinase, dTMP kinase
Recommended products
Rabbit Recombinant Monoclonal DTYMK antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10163
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249166 is the carrier-free version of Anti-DTYMK antibody [EPR10163] ab154867.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--western-blot-img105675.jpg "Western blot - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
Western blot - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)
This data was developed using Anti-DTYMK antibody [EPR10163] ab154867, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-DTYMK antibody [EPR10163] (Anti-DTYMK antibody [EPR10163] ab154867) at 1/1000 dilution
Lane 1: Raji cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Predicted band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126787.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)
This data was developed using Anti-DTYMK antibody [EPR10163] ab154867, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling DTYMK with ab137875 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunocytochemistry-immunofluorescence-img117455.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
Immunocytochemistry/ Immunofluorescence - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)
This data was developed using Anti-DTYMK antibody [EPR10163] ab154867, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of HeLa cells labeling DTYMK with Anti-DTYMK antibody [EPR10163] ab154867 at 1/100.
![Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--flow-cytometry-intracellular-img175933.jpg "Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
Flow Cytometry (Intracellular) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)
This data was developed using Anti-DTYMK antibody [EPR10163] ab154867, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DTYMK with purified Anti-DTYMK antibody [EPR10163] ab154867 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119630.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)
This data was developed using Anti-DTYMK antibody [EPR10163] ab154867, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using Anti-DTYMK antibody [EPR10163] ab154867 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104572.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)
This data was developed using Anti-DTYMK antibody [EPR10163] ab154867, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin embedded Human prostate hyperplasia tissue using Anti-DTYMK antibody [EPR10163] ab154867 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126345.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)
This data was developed using Anti-DTYMK antibody [EPR10163] ab154867, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using Anti-DTYMK antibody [EPR10163] ab154867 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104572.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166), expandable thumbnail](https://content.abcam.com/products/images/dtymk-antibody-epr10163-bsa-and-azide-free-ab249166--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126345.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTYMK antibody [EPR10163] - BSA and Azide free (ab249166)")
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