AB176727

Anti-DUBA antibody

Name: Anti-DUBA antibody (ab176727) | Abcam
Brand: Abcam Limited
SKU: AB176727

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(5 Publications)

Rabbit Polyclonal DUBA antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human OTUD5 aa 150-500.

View Alternative Names

OTU domain-containing protein 5, Deubiquitinating enzyme A, DUBA, OTUD5

2 Images

Supplier Data

Immunoprecipitation - Anti-DUBA antibody (AB176727)

Western blot of immunoprecipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling DUBA using ab176727 used for WB at 1 μg/ml and used for IP at 3 μg/mg lysate, using chemiluminescence with exposure times of 10 seconds.

All lanes:

Immunoprecipitation - Anti-DUBA antibody (ab176727)

Predicted band size: 61 kDa

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Supplier Data

Western blot - Anti-DUBA antibody (AB176727)

Chemiluminescence with exposure times of 3 minutes.

All lanes:

Western blot - Anti-DUBA antibody (ab176727) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 61 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human OTUD5 aa 150-500. The exact immunogen used to generate this antibody is proprietary information.

Q96G74

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinating enzyme that functions as a negative regulator of the innate immune system (PubMed : 17991829, PubMed : 22245969, PubMed : 23827681, PubMed : 33523931). Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 22245969). Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (PubMed : 22245969). Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production (PubMed : 17991829). Controls neuroectodermal differentiation through cleaving 'Lys-48'-linked ubiquitin chains to counteract degradation of select chromatin regulators such as ARID1A, HDAC2 and HCF1 (PubMed : 33523931). Acts as a positive regulator of mTORC1 and mTORC2 signaling following phosphorylation by MTOR : acts by mediating deubiquitination of BTRC, leading to its stability (PubMed : 33110214).

See full target information OTUD5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406700 PubMed39721018

2024

OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Song,Tengfei Liu,Lu Yu,Qiuran Ji,Xin Guo,Runzhe Zong,Yiquan Li,Gan Huang,Qidi Xue,Qingyi Fu,Bingyu Liu,Yi Zheng,Lin Chen,Chengjiang Gao,Huiqing Liu

Cell death and differentiation 31:753-767 PubMed38605168

2024

OTUD5 promotes the inflammatory immune response by enhancing MyD88 oligomerization and Myddosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxing Liu,Jiahua Yuan,Yuling Zhang,Fei Qin,Xuemei Bai,Wanwei Sun,Tian Chen,Feng Liu,Yi Zheng,Xiaopeng Qi,Wei Zhao,Bingyu Liu,Chengjiang Gao

Bosnian journal of basic medical sciences 22:901-911 PubMed35765958

2022

Mechanism of OTUD5 in non-small cell lung cancer cell proliferation, invasion, and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Xuebing Li,Baohua Lu,Lina Zhang,Jing Yang,Yurong Cheng,Dong Yan

Cell death and differentiation 28:900-914 PubMed33110214

2020

Deubiquitinase OTUD5 is a positive regulator of mTORC1 and mTORC2 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hwa Cho,Kidae Kim,Sung Ah Kim,Sungryul Park,Bi-Oh Park,Jong-Hwan Kim,Seon-Young Kim,Min Jee Kwon,Myeong Hoon Han,Sung Bae Lee,Byoung Chul Park,Sung Goo Park,Jeong-Hoon Kim,Sunhong Kim

The Journal of biological chemistry 292:11804-11814 PubMed28559278

2017

The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hwa Cho,Sung Ah Kim,Yeon-Soo Seo,Sung Goo Park,Byoung Chul Park,Jeong-Hoon Kim,Sunhong Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DUBA antibody

Immunoprecipitation - Anti-DUBA antibody (AB176727)

Western blot - Anti-DUBA antibody (AB176727)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise