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AB185352

Anti-DUBA2 antibody

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(2 Publications)

Rabbit Polyclonal DUBA2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OTUD6A aa 50-150.

View Alternative Names

DUBA2, OTUD6A, OTU domain-containing protein 6A, DUBA-2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUBA2 antibody (AB185352)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUBA2 antibody (AB185352)

Immunohistochemistry analysis of human testis tissue labeling DUBA2 (high expression) with ab185352 at a 1/2500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUBA2 antibody (AB185352)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUBA2 antibody (AB185352)

Immunohistochemistry analysis of human endometrium tissue labeling DUBA2 (low expression) with ab185352 at a 1/2500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-DUBA2 antibody (AB185352)
  • WB

Supplier Data

Western blot - Anti-DUBA2 antibody (AB185352)

All lanes:

Western blot - Anti-DUBA2 antibody (ab185352) at 0.4 µg/mL

Lane 1:

Control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

DUBA2 over-expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human OTUD6A aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q7L8S5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinating enzyme that hydrolyzes 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains.
See full target information OTUD6A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 82:3143-3157 PubMed35705526

2022

MAB21L4 Deficiency Drives Squamous Cell Carcinoma via Activation of RET.

Applications

Unspecified application

Species

Unspecified reactive species

Ankit Srivastava,Cristina Tommasi,Dane Sessions,Angela Mah,Tomas Bencomo,Jasmine M Garcia,Tiffany Jiang,Michael Lee,Joseph Y Shen,Lek Wei Seow,Audrey Nguyen,Kimal Rajapakshe,Cristian Coarfa,Kenneth Y Tsai,Vanessa Lopez-Pajares,Carolyn S Lee

Molecular oncology 14:3169-3183 PubMed33070427

2020

Deubiquitinase OTUD6A promotes proliferation of cancer cells via regulating Drp1 stability and mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Le Shi,Jing Liu,Yunhua Peng,Jinfang Zhang,Xiangpeng Dai,Shuangxi Zhang,Yongyao Wang,Jiankang Liu,Jiangang Long
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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