AB181262

Anti-DUS2L antibody [EPR14042]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DUS2L antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DUS2L, DUS2, tRNA-dihydrouridine(20) synthase [NAD(P)+]-like, Dihydrouridine synthase 2, Up-regulated in lung cancer protein 8, tRNA-dihydrouridine synthase 2-like, URLC8, hDUS2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-DUS2L antibody [EPR14042] (AB181262)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DUS2L antibody [EPR14042] (AB181262)

Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling DUS2L with ab181262 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Flow Cytometry (Intracellular) - Anti-DUS2L antibody [EPR14042] (AB181262)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DUS2L antibody [EPR14042] (AB181262)

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling DUS2L with ab181262 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] (AB181262)

Western blot analysis of immunoprecipitation pellet from A549 cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181262 at 1/50 dilution.
Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] (ab181262)

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-DUS2L antibody [EPR14042] (AB181262)

All lanes:

Western blot - Anti-DUS2L antibody [EPR14042] (ab181262) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

Carrier free

Anti-DUS2L antibody [EPR14042] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14042

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (PubMed : 15994936, PubMed : 26429968, PubMed : 30149704, PubMed : 34798057, PubMed : 38680565). Specifically modifies U20 in cytoplasmic tRNAs (PubMed : 38680565). Activity depends on the presence of guanosine at position 19 in the tRNA substrate (PubMed : 38680565). Negatively regulates the activation of EIF2AK2/PKR (PubMed : 18096616).

See full target information DUS2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 18:4239-4246 PubMed30221720

2018

Long non‑coding RNA ferritin heavy polypeptide 1 pseudogene 3 controls glioma cell proliferation and apoptosis via regulation of the microRNA‑224‑5p/tumor protein D52 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Zhang,Ying Li,Jing Wang,Ping Lei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DUS2L antibody [EPR14042]

Immunocytochemistry/ Immunofluorescence - Anti-DUS2L antibody [EPR14042] (AB181262)

Flow Cytometry (Intracellular) - Anti-DUS2L antibody [EPR14042] (AB181262)

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] (AB181262)

Western blot - Anti-DUS2L antibody [EPR14042] (AB181262)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise