JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250475

Anti-DUS2L antibody [EPR14042] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal DUS2L antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

DUS2L, DUS2, tRNA-dihydrouridine(20) synthase [NAD(P)+]-like, Dihydrouridine synthase 2, Up-regulated in lung cancer protein 8, tRNA-dihydrouridine synthase 2-like, URLC8, hDUS2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (AB250475)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (AB250475)

This data was developed using ab181262, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling DUS2L with ab181262 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Flow Cytometry (Intracellular) - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (AB250475)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (AB250475)

This data was developed using ab181262, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling DUS2L with ab181262 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (AB250475)
  • IP

Supplier Data

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (AB250475)

This data was developed using ab181262, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from A549 cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181262 at 1/50 dilution.
Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-DUS2L antibody [EPR14042] (<a href='/en-us/products/primary-antibodies/dus2l-antibody-epr14042-ab181262'>ab181262</a>)

Predicted band size: 55 kDa

false

Western blot - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (AB250475)
  • WB

Supplier Data

Western blot - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (AB250475)

This data was developed using ab181262, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DUS2L antibody [EPR14042] (<a href='/en-us/products/primary-antibodies/dus2l-antibody-epr14042-ab181262'>ab181262</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14042

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB259990

Anti-YTHDC1 antibody [EPR21821-213]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-YTHDC1 antibody [EPR21821-213] (AB259990)

  • 0
  • 0
Primary Antibodies

AB243656

Anti-LATS1/WARTS antibody [EPR23057-116]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-LATS1/WARTS antibody [EPR23057-116] (AB243656)

  • 0
  • 0
Primary Antibodies

AB128868

Anti-SF3A1 antibody [EPR7666]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A1 antibody [EPR7666] (AB128868)

  • 0
  • 0
Primary Antibodies

AB126605

Anti-FTO antibody [EPR6894]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-FTO antibody [EPR6894] (AB126605)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250475 is the carrier-free version of ab181262.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (PubMed : 15994936, PubMed : 26429968, PubMed : 30149704, PubMed : 34798057, PubMed : 38680565). Specifically modifies U20 in cytoplasmic tRNAs (PubMed : 38680565). Activity depends on the presence of guanosine at position 19 in the tRNA substrate (PubMed : 38680565). Negatively regulates the activation of EIF2AK2/PKR (PubMed : 18096616).
See full target information DUS2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com