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Rabbit Recombinant Monoclonal DUS2 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Recombinant fragment - Human samples.

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Images

Western blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (AB323536), expandable thumbnail
  • Dot Blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (AB323536), expandable thumbnail
  • Western blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (AB323536), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PDot
Human
Tested
Not recommended
Expected
Mouse
Tested
Not recommended
Expected
Recombinant fragment - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Species

Mouse

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info
1/1000
Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Dephosphorylates both phosphorylated Thr and Tyr residues in MAPK1, and dephosphorylation of phosphotyrosine is slightly faster than that of phosphothreonine (PubMed:8107850). Can dephosphorylate MAPK1 (By similarity).

Alternative names

PAC1, DUSP2, Dual specificity protein phosphatase 2, Dual specificity protein phosphatase PAC-1

Rabbit Recombinant Monoclonal DUS2 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Recombinant fragment - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29656-23

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536), expandable thumbnail

    Western blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression of DUSP2 is upregulated in response to LPS treatment (PMID: 8649402).

    The identity of the higher MW band at approximately 75 kDa (in lanes 1-4) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536) at 1/1000 dilution

    Lane 1: Untreated Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 50 µg with NFDM/TBST

    Lane 2: Raji treated with 20ug/ml LPS for 4h whole cell lysate at 50 µg with NFDM/TBST

    Lane 3: Untreated Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 50 µg with NFDM/TBST

    Lane 4: Jurkat treated with 1ug/ml PHA for and 40ng/ml PMA for 3h whole cell lysate at 50 µg with NFDM/TBST

    Lane 5: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 35 kDa, 36 kDa

    Exposure time: 114s

  • Dot Blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536), expandable thumbnail

    Dot Blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536)

    Dot blot analysis of DUSP2/PAC1 using ab323536 at 1:1000 (0.507 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:20,000 dilution.

    Lane1: Myc-tagged human DUSP2 recombinant protein 10ng

    Lane2: Myc-tagged human DUSP1 recombinant protein 10ng

    Lane3: Myc-tagged human DUSP5 recombinant protein 10ng

    Lane4: Myc-tagged human DUSP4 recombinant protein 50ng

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Dot Blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536) at 1/1000 dilution

    Lane 1: Myc-tagged human DUSP2 recombinant protein at 10 ng

    Lane 2: Myc-tagged human DUSP1 recombinant protein at 10 ng

    Lane 3: Myc-tagged human DUSP5 recombinant protein at 10 ng

    Lane 4: Myc-tagged human DUSP4 recombinant protein at 50 ng

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 180s

  • Western blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536), expandable thumbnail

    Western blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: MEF, F9

    The expression of DUSP2 is upregulated in response to LPS treatment (PMID: 8649402).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-DUSP2/PAC1 antibody [EPR29656-23] (ab323536) at 1/1000 dilution

    Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) whole cell lysate at 50 µg with NFDM/TBST

    Lane 2: Untreated LADMAC (mouse bone marrow monocyte macrophage) whole cell lysate at 50 µg with NFDM/TBST

    Lane 3: LADMAC treated with 100 ng/ml LPS for 24h whole cell lysate at 50 µg with NFDM/TBST

    Lane 4: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 50 µg with NFDM/TBST

    Lane 5: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg with NFDM/TBST

    Lane 6: MEF (mouse embryo fibroblast) whole cell lysate at 50 µg with NFDM/TBST

    Lane 7: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 35 kDa, 36 kDa

    Exposure time: 114s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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