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AB327773

Anti-DUSP22 antibody [3D3] - BSA and Azide free

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Mouse Monoclonal DUSP22 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DUSP22.
2 Images
Western blot - Anti-DUSP22 antibody [3D3] - BSA and Azide free (AB327773)
  • WB

Supplier Data

Western blot - Anti-DUSP22 antibody [3D3] - BSA and Azide free (AB327773)

Western Blot detection against Immunogen ab327773 - GST tagged partial DUSP22 construct (33.77 KDa).

In Western blot, Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody was used at 1/2500 - 1/5000

Blocking and diluting buffer and concentration : 5% non-fat milk/PBST (0.2%).

All lanes:

Western blot - Anti-DUSP22 antibody [3D3] - BSA and Azide free (ab327773)

All lanes:

GST tagged partial DUSP22 construct

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody

Observed band size: 33.77 kDa

false

Western blot - Anti-DUSP22 antibody [3D3] - BSA and Azide free (AB327773)
  • WB

Supplier Data

Western blot - Anti-DUSP22 antibody [3D3] - BSA and Azide free (AB327773)

Western Blot analysis of DUSP22 expression in transfected 293T cell line by Anti-DUSP22 antibody [3D3] - BSA and Azide free (ab327773).

In Western blot, Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody was used at 1/2500 - 1/5000

Blocking and diluting buffer and concentration : 5% non-fat milk/PBST (0.2%).

All lanes:

Western blot - Anti-DUSP22 antibody [3D3] - BSA and Azide free (ab327773)

Lane 1:

DUSP22 transfected 293T lysate

Lane 2:

Non-transfected 293T lysate

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate

Observed band size: 20.9 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3D3

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human DUSP22.

Q9NRW4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

Source: Hybridoma culture supernatant.
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: 100% 1X PBS
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues (PubMed : 24714587, PubMed : 38225265). Activates the JNK signaling pathway (PubMed : 11717427). Inhibits T-cell receptor signaling and T-cell mediated immune responses, acting, at least in part, by inducing degradation of E3 ubiquitin ligase UBR2 (PubMed : 24714587, PubMed : 38225265). Dephosphorylates and thereby induces 'Lys-48'-linked ubiquitination of UBR2, leading to proteasomal degradation of UBR2 (PubMed : 38225265). Dephosphorylates and thereby inactivates tyrosine kinase LCK (PubMed : 24714587). Inhibits UBR2-mediated 'Lys-63'-linked ubiquitination of LCK (PubMed : 38225265). May play a role in B-cell receptor (BCR) signaling and B-cell function (By similarity).
See full target information DUSP22

Alternative Names

JSP1, LMWDSP2, MKPX, DUSP22, Dual specificity protein phosphatase 22, JNK pathway associated phosphatase, JNK-stimulatory phosphatase-1, Low molecular weight dual specificity phosphatase 2, Mitogen-activated protein kinase phosphatase x, JKAP, JSP-1, LMW-DSP2, MAP kinase phosphatase x, MKP-x
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Product promise

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