Rabbit Recombinant Monoclonal DUSP9 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (AB251189), expandable thumbnail](https://content.abcam.com/products/images/dusp9-antibody-epr15541-bsa-and-azide-free-ab251189--western-blot-img149162.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (AB251189)")
![Immunocytochemistry/ Immunofluorescence - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (AB251189), expandable thumbnail](https://content.abcam.com/products/images/dusp9-antibody-epr15541-bsa-and-azide-free-ab251189--immunocytochemistry-immunofluorescence-img14727.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (AB251189)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Human | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Inactivates MAP kinases. Has a specificity for the ERK family.
Alternative names
MKP4, DUSP9, Dual specificity protein phosphatase 9, Mitogen-activated protein kinase phosphatase 4, MAP kinase phosphatase 4, MKP-4
Recommended products
Rabbit Recombinant Monoclonal DUSP9 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15541
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251189 is the carrier-free version of Anti-DUSP9 antibody [EPR15541] ab194355.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (ab251189), expandable thumbnail](https://content.abcam.com/products/images/dusp9-antibody-epr15541-bsa-and-azide-free-ab251189--western-blot-img149162.jpg "Western blot - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (ab251189)")
Western blot - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (ab251189)
This data was developed using Anti-DUSP9 antibody [EPR15541] ab194355, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-DUSP9 antibody [EPR15541] (Anti-DUSP9 antibody [EPR15541] ab194355) at 1/2000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: 293 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (ab251189), expandable thumbnail](https://content.abcam.com/products/images/dusp9-antibody-epr15541-bsa-and-azide-free-ab251189--immunocytochemistry-immunofluorescence-img14727.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (ab251189)")
Immunocytochemistry/ Immunofluorescence - Anti-DUSP9 antibody [EPR15541] - BSA and Azide free (ab251189)
This data was developed using Anti-DUSP9 antibody [EPR15541] ab194355, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling DUSP9 with Anti-DUSP9 antibody [EPR15541] ab194355 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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