JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB137097

Anti-dUTPase antibody [EPR9608(B)]

4

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal dUTPase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

dUTPase, dUTP pyrophosphatase, DUT

3 Images
Flow Cytometry (Intracellular) - Anti-dUTPase antibody [EPR9608(B)] (AB137097)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-dUTPase antibody [EPR9608(B)] (AB137097)

Flow Cytometry analysis of SW620 (Human colorectal adenocarcinoma epithelial cell) cells labeling dUTPase with purified ab137097 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9608(B)] (AB137097)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9608(B)] (AB137097)

Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling dUTPase with ab137097 at 1/250 dilution.

Western blot - Anti-dUTPase antibody [EPR9608(B)] (AB137097)
  • WB

Unknown

Western blot - Anti-dUTPase antibody [EPR9608(B)] (AB137097)

All lanes:

Western blot - Anti-dUTPase antibody [EPR9608(B)] (ab137097) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9608(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme deoxyuridine triphosphatese also known as dUTPase hydrolyzes dUTP to dUMP and inorganic pyrophosphate. It prevents the incorporation of uracil into DNA which can lead to mutations. The molecular mass of human dUTPase is about 18-20 kDa. It is widely expressed in both prokaryotic and eukaryotic cells including places like the thymus and spleen where DNA synthesis is very active. Alternative names such as DUT or dUTP pyrophosphatase are often used to refer to this enzyme.
Biological function summary

DUTPase maintains nucleotide pool balance and genome integrity. By converting dUTP to dUMP it provides substrates for thymidylate synthase to produce thymidine triphosphate (dTTP) which is essential for DNA replication and repair. dUTPase is not typically part of a larger protein complex but cooperates with other enzymes in nucleotide metabolism. Its activity protects cells from apoptosis that might occur due to extensive uracil misincorporation.

Pathways

DUTPase plays a role in nucleotide metabolism and one-carbon metabolism pathways. It regulates the pyrimidine metabolism pathway ensuring sufficient dTTP levels for DNA synthesis. dUTPase interacts indirectly with proteins like thymidylate synthase and dihydrofolate reductase through these pathways. These interactions aid in maintaining a steady supply of nucleotides important for DNA replication and repair.

Impairments in dUTPase function can relate to cancer and immunodeficiency disorders. In some cancers altered dUTPase expression affects the sensitivity to chemotherapeutic agents particularly those targeting thymidylate synthesis. In certain immunodeficiencies dUTPase malfunction leads to increased uracil incorporation compromising DNA integrity. The enzyme connects with proteins such as thymidylate synthase in these diseases due to its role in managing nucleotide pools critical for cell proliferation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis (PubMed : 17880943, PubMed : 8631816, PubMed : 8805593). Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor (By similarity). Essential for embryonic development (By similarity).
See full target information DUT
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:2036-2052.e7 PubMed38688279

2024

Unprocessed genomic uracil as a source of DNA replication stress in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sneha Saxena,Christopher S Nabel,Turner W Seay,Parasvi S Patel,Ajinkya S Kawale,Caroline R Crosby,Helene Tigro,Eugene Oh,Matthew G Vander Heiden,Aaron N Hata,Zucai Suo,Lee Zou

Molecular cell 81:767-783.e11 PubMed33333017

2020

Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD.

Applications

Unspecified application

Species

Unspecified reactive species

Graeme Hewitt,Valerie Borel,Sandra Segura-Bayona,Tohru Takaki,Phil Ruis,Roberto Bellelli,Laura C Lehmann,Lucia Sommerova,Aleksandra Vancevska,Antonia Tomas-Loba,Kang Zhu,Christopher Cooper,Kasper Fugger,Harshil Patel,Robert Goldstone,Deborah Schneider-Luftman,Ellie Herbert,Gordon Stamp,Rachel Brough,Stephen Pettitt,Christopher J Lord,Stephen C West,Ivan Ahel,Dragana Ahel,J Ross Chapman,Sebastian Deindl,Simon J Boulton

Cancer research 74:5948-54 PubMed25145669

2014

Synergy between the NAMPT inhibitor GMX1777(8) and pemetrexed in non-small cell lung cancer cells is mediated by PARP activation and enhanced NAD consumption.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Chan,Michel Gravel,Alexandre Bramoullé,Gaëlle Bridon,Daina Avizonis,Gordon C Shore,Anne Roulston
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB229122

Anti-dUTPase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dUTPase antibody (AB229122)

  • 0
  • 0
Primary Antibodies

AB137102

Anti-dUTPase antibody [EPR9607(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (AB137102)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB171084

Anti-Mad2L1 antibody [EPR9852]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Mad2L1 antibody [EPR9852] (AB171084)

  • 0
  • 0
Primary Antibodies

AB156587

Anti-SNRPF antibody [EPR10389(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SNRPF antibody [EPR10389(B)] (AB156587)

  • 0
  • 0
Primary Antibodies

AB172607

Anti-RECQ1 antibody [EPR8049(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody [EPR8049(2)] (AB172607)

  • 0
  • 0
Primary Antibodies

AB124670

Anti-HMGB2 antibody [EPR6301]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB2 antibody [EPR6301] (AB124670)

  • 4
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com