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Rabbit Polyclonal DUX4 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DUX4 aa 1-100.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Dux4 antibody (AB220987), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human DUX4 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBX2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Associated Products

Select an associated product type

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Target data

Function

Isoform 1Transcription factor that is selectively and transiently expressed in cleavage-stage embryos (PubMed:28459457). Binds to double-stranded DNA elements with the consensus sequence 5'-TAATCTAATCA-3' (PubMed:28459454, PubMed:28459457, PubMed:29572508, PubMed:30315230, PubMed:30540931). Binds to chromatin containing histone H3 acetylated at 'Lys-27' (H3K27ac) and promotes deacetylation of H3K27ac. In parallel, binds to chromatin that lacks histone H3 acetylation at 'Lys-27' (H3K27ac) and recruits EP300 and CREBBP to promote acetylation of histone H3 at 'Lys-27' at new sites (PubMed:26951377). Involved in transcriptional regulation of numerous genes, primarily as transcriptional activator, but also mediates repression of a set of target genes (PubMed:17984056, PubMed:26951377, PubMed:27378237, PubMed:28459454, PubMed:28459457, PubMed:29572508, PubMed:29618456, PubMed:30540931). Promotes expression of ZSCAN4 and KDM4E, two proteins with essential roles during early embryogenesis (PubMed:26951377, PubMed:27378237, PubMed:28459457, PubMed:29618456). Promotes nuclear translocation of CTNNB1/beta-catenin and its subsequent activation of target genes (PubMed:36158201). Heterologous expression in cultured embryonic stem cells mediates transcription of HERVL retrotransposons and transcripts derived from ACRO1 and HSATII satellite repeats (PubMed:28459457). May activate expression of PITX1 (PubMed:17984056). May regulate microRNA (miRNA) expression (PubMed:24145033). Inappropriate expression can inhibit myogenesis and promote apoptosis (PubMed:26951377, PubMed:28935672, PubMed:29618456).Isoform 2Probably inactive as a transcriptional activator, due to the absence of the C-terminal region that is important for transcriptional activation. Can inhibit transcriptional activation mediated by isoform 1. Heterologous expression of isoform 2 has no deleterious effect on cell survival.

Alternative names

Recommended products

Rabbit Polyclonal DUX4 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DUX4 aa 1-100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human DUX4 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBX2
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Dux4 also known as Double homeobox 4 is a transcription factor with an important role in gene regulation. It has a molecular mass of approximately 52 kDa and is expressed mainly in germline and early embryonic tissues but it is usually silenced in adults. Dux4 contains a double homeobox domain which facilitates its ability to bind DNA sequences and regulate target gene expression. Despite its limited expression in adult tissues Dux4 is present in low levels in certain muscle tissues.

Biological function summary

Dux4 regulates a wide range of genes associated with muscle differentiation and apoptosis. It is not typically part of a known protein complex but acts independently to influence gene activity. Its function as a transcription factor impacts muscle cell differentiation by activating various myogenic and apoptotic genes. This regulatory activity plays a major role in normal muscle function and development in embryos.

Pathways

Dux4 plays a significant role in the Wnt signaling and beta-catenin pathways. These pathways are important for tissue development and cellular proliferation. Dux4 interacts with proteins like beta-catenin to influence cell growth and differentiation. Inappropriate activation of these pathways due to Dux4 involvement can result in dysregulated cell growth impacting muscle tissue integrity.

Associated diseases and disorders

The inappropriate expression of Dux4 is related to facioscapulohumeral muscular dystrophy (FSHD) and cancer. FSHD involves the misexpression of Dux4 in muscle tissues leading to muscle weakness and degeneration. In cancer Dux4 misexpression can contribute to tumor development and progression by disrupting normal cell differentiation processes. Dux4's interaction with proteins involved in these conditions such as beta-catenin helps to elucidate its role in disease pathology.

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-Dux4 antibody (ab220987), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Dux4 antibody (ab220987)

    Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling Dux4 with ab220987 at 4 μg/ml (green).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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