AB124699

Anti-Dux4 antibody [E5-5]

Name: Anti-Dux4 antibody [E5-5] (ab124699) | Abcam
Brand: Abcam Limited
SKU: AB124699
Price: 4820 USD
Availability: InStock
Rating: 3 (3 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(3 Reviews)

(38 Publications)

Anti-Dux4 antibody [E5-5] (ab124699) is a rabbit monoclonal antibody detecting Dux4 in Western Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

View Alternative Names

DUX10, DUX4, Double homeobox protein 4, Double homeobox protein 10

3 Images

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Western blot - Anti-Dux4 antibody [E5-5] (AB124699)

All lanes:

Western blot - Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

HT1080 (human fibrosarcoma cell line) cell lysate at 10 µg

Lane 3:

PC-3 (human prostate adenocarcinoma cell line) cell lysate at 10 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 45 kDa,52 kDa

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Lab

Western blot - Anti-Dux4 antibody [E5-5] (AB124699)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

Lane 1:

HT1080 (human fibrosarcoma cell line) whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

Saos-2 (human osteosarcoma cell line) whole cell lysate at 20 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Lab

Western blot - Anti-Dux4 antibody [E5-5] (AB124699)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM /TBST The expression profile observed in HFF-1 is consistent with the literature (PMID : 30420784). Negative control : HFF-1 (PMID : 30420784)

Lanes 1 - 5:

Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/dux4-antibody-e5-5-bsa-and-azide-free-ab216893'>ab216893</a>) at 1/1000 dilution

Lanes 1 - 5:

Western blot - Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

HFF-1 (Human Skin fibroblast) whole cell lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Exposure time: 60s

HRP

HRP Anti-Dux4 antibody [E5-5]
Carrier free

Anti-Dux4 antibody [E5-5] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E5-5

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10 - 1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Dux4 antibody [E5-5] (ab124699) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Dux4?
Anti-Dux4 [E5-5] (ab124699) specifically detects a band for Dux4 (UniProt: Q9UBX2) at a molecular weight of 45kDa.

Trusted by the scientific community
Anti-Dux4 [E5-5] (ab124699) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [E5-5] also available for your convenience: ab124699, HRP - ab208840, Carrier free - ab216893

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dux4 also known as Double homeobox 4 is a transcription factor with an important role in gene regulation. It has a molecular mass of approximately 52 kDa and is expressed mainly in germline and early embryonic tissues but it is usually silenced in adults. Dux4 contains a double homeobox domain which facilitates its ability to bind DNA sequences and regulate target gene expression. Despite its limited expression in adult tissues Dux4 is present in low levels in certain muscle tissues.

Biological function summary

Dux4 regulates a wide range of genes associated with muscle differentiation and apoptosis. It is not typically part of a known protein complex but acts independently to influence gene activity. Its function as a transcription factor impacts muscle cell differentiation by activating various myogenic and apoptotic genes. This regulatory activity plays a major role in normal muscle function and development in embryos.

Pathways

Dux4 plays a significant role in the Wnt signaling and beta-catenin pathways. These pathways are important for tissue development and cellular proliferation. Dux4 interacts with proteins like beta-catenin to influence cell growth and differentiation. Inappropriate activation of these pathways due to Dux4 involvement can result in dysregulated cell growth impacting muscle tissue integrity.

The inappropriate expression of Dux4 is related to facioscapulohumeral muscular dystrophy (FSHD) and cancer. FSHD involves the misexpression of Dux4 in muscle tissues leading to muscle weakness and degeneration. In cancer Dux4 misexpression can contribute to tumor development and progression by disrupting normal cell differentiation processes. Dux4's interaction with proteins involved in these conditions such as beta-catenin helps to elucidate its role in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Transcription factor that is selectively and transiently expressed in cleavage-stage embryos (PubMed : 28459457). Binds to double-stranded DNA elements with the consensus sequence 5'-TAATCTAATCA-3' (PubMed : 28459454, PubMed : 28459457, PubMed : 29572508, PubMed : 30315230, PubMed : 30540931). Binds to chromatin containing histone H3 acetylated at 'Lys-27' (H3K27ac) and promotes deacetylation of H3K27ac. In parallel, binds to chromatin that lacks histone H3 acetylation at 'Lys-27' (H3K27ac) and recruits EP300 and CREBBP to promote acetylation of histone H3 at 'Lys-27' at new sites (PubMed : 26951377). Involved in transcriptional regulation of numerous genes, primarily as transcriptional activator, but also mediates repression of a set of target genes (PubMed : 17984056, PubMed : 26951377, PubMed : 27378237, PubMed : 28459454, PubMed : 28459457, PubMed : 29572508, PubMed : 29618456, PubMed : 30540931). Promotes expression of ZSCAN4 and KDM4E, two proteins with essential roles during early embryogenesis (PubMed : 26951377, PubMed : 27378237, PubMed : 28459457, PubMed : 29618456). Promotes nuclear translocation of CTNNB1/beta-catenin and its subsequent activation of target genes (PubMed : 36158201). Heterologous expression in cultured embryonic stem cells mediates transcription of HERVL retrotransposons and transcripts derived from ACRO1 and HSATII satellite repeats (PubMed : 28459457). May activate expression of PITX1 (PubMed : 17984056). May regulate microRNA (miRNA) expression (PubMed : 24145033). Inappropriate expression can inhibit myogenesis and promote apoptosis (PubMed : 26951377, PubMed : 28935672, PubMed : 29618456).. Isoform 2. Probably inactive as a transcriptional activator, due to the absence of the C-terminal region that is important for transcriptional activation. Can inhibit transcriptional activation mediated by isoform 1. Heterologous expression of isoform 2 has no deleterious effect on cell survival.

See full target information DUX4

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadt5356 PubMed40333962

2025

DUX4 activates common and context-specific intergenic transcripts and isoforms.

Applications

Unspecified application

Species

Unspecified reactive species

Dongxu Zheng,Anita van den Heuvel,Judit Balog,Iris M Willemsen,Susan Kloet,Stephen J Tapscott,Ahmed Mahfouz,Silvère M van der Maarel

International journal of molecular sciences 25: PubMed39000102

2024

Systemic Pharmacotherapeutic Treatment of the ACTA1-MCM/FLExDUX4 Preclinical Mouse Model of FSHD.

Applications

Unspecified application

Species

Unspecified reactive species

Ngoc Lu-Nguyen,Stuart Snowden,Linda Popplewell,Alberto Malerba

Clinical and translational medicine 13:e1514 PubMed38115701

2023

Transcription factor 12-mediated self-feedback regulatory mechanism is required in DUX4 fusion leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Li,Minghao Jiang,Junfei Wang,Zhiyi Zhuo,Shiyan Zhang,Yangxia Tan,Weiguo Hu,Hao Zhang,Guoyu Meng

Cell death & disease 14:749 PubMed37973788

2023

Flavones provide resistance to DUX4-induced toxicity via an mTor-independent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Justin Cohen,Shushu Huang,Katherine E Koczwara,Kristen T Woods,Vincent Ho,Keryn G Woodman,Jack L Arbiser,Katelyn Daman,Monkol Lek,Charles P Emerson,Alec M DeSimone

Science advances 9:eabq7744 PubMed36800423

2023

DUX4 double whammy: The transcription factor that causes a rare muscular dystrophy also kills the precursors of the human nose.

Applications

Unspecified application

Species

Unspecified reactive species

Kaoru Inoue,Hamed Bostan,MaKenna R Browne,Owen F Bevis,Carl D Bortner,Steven A Moore,Aaron A Stence,Negin P Martin,Shih-Heng Chen,Adam B Burkholder,Jian-Liang Li,Natalie D Shaw

Disease models & mechanisms 15: PubMed36196640

2022

DUX4 expression activates JNK and p38 MAP kinases in myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher M Brennan,Abby S Hill,Michael St Andre,Xianfeng Li,Vijaya Madeti,Susanne Breitkopf,Seth Garren,Liang Xue,Tamara Gilbert,Angela Hadjipanayis,Mara Monetti,Charles P Emerson,Robert Moccia,Jane Owens,Nicolas Christoforou

BMC research notes 15:163 PubMed35538497

2022

Proximity ligation assay to detect DUX4 protein in FSHD1 muscle: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Mary Lou Beermann,Sachiko Homma,Jeffrey Boone Miller

Cell death & disease 13:405 PubMed35468884

2022

Antiapoptotic Protein FAIM2 is targeted by miR-3202, and DUX4 via TRIM21, leading to cell death and defective myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hossam A N Soliman,Erik A Toso,Inas E Darwish,Samia M Ali,Michael Kyba

iScience 25:104137 PubMed35402882

2022

is a multifunctional factor priming human embryonic genome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Sanna Vuoristo,Shruti Bhagat,Christel Hydén-Granskog,Masahito Yoshihara,Lisa Gawriyski,Eeva-Mari Jouhilahti,Vipin Ranga,Mahlet Tamirat,Mikko Huhtala,Ida Kirjanov,Sonja Nykänen,Kaarel Krjutškov,Anastassius Damdimopoulos,Jere Weltner,Kosuke Hashimoto,Gaëlle Recher,Sini Ezer,Priit Paluoja,Pauliina Paloviita,Yujiro Takegami,Ai Kanemaru,Karolina Lundin,Tomi T Airenne,Timo Otonkoski,Juha S Tapanainen,Hideya Kawaji,Yasuhiro Murakawa,Thomas R Bürglin,Markku Varjosalo,Mark S Johnson,Timo Tuuri,Shintaro Katayama,Juha Kere

Biology open 11: PubMed35191484

2022

Downstream events initiated by expression of FSHD-associated DUX4: Studies of nucleocytoplasmic transport, γH2AX accumulation, and Bax/Bak-dependence.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel F Masteika,Anvitha Sathya,Sachiko Homma,Bess M Miller,Frederick M Boyce,Jeffrey Boone Miller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dux4 antibody [E5-5]

Western blot - Anti-Dux4 antibody [E5-5] (AB124699)

Western blot - Anti-Dux4 antibody [E5-5] (AB124699)

Western blot - Anti-Dux4 antibody [E5-5] (AB124699)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

Product promise