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Rabbit Recombinant Monoclonal DUX4 antibody. Suitable for WB and reacts with Human samples. Cited in 34 publications.


Images

Western blot - Anti-Dux4 antibody [E5-5] (AB124699), expandable thumbnail
  • Western blot - Anti-Dux4 antibody [E5-5] (AB124699), expandable thumbnail
  • Western blot - Anti-Dux4 antibody [E5-5] (AB124699), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Isoform 1. Transcription factor that is selectively and transiently expressed in cleavage-stage embryos (PubMed:28459457). Binds to double-stranded DNA elements with the consensus sequence 5'-TAATCTAATCA-3' (PubMed:28459454, PubMed:28459457, PubMed:29572508, PubMed:30315230, PubMed:30540931). Binds to chromatin containing histone H3 acetylated at 'Lys-27' (H3K27ac) and promotes deacetylation of H3K27ac. In parallel, binds to chromatin that lacks histone H3 acetylation at 'Lys-27' (H3K27ac) and recruits EP300 and CREBBP to promote acetylation of histone H3 at 'Lys-27' at new sites (PubMed:26951377). Involved in transcriptional regulation of numerous genes, primarily as transcriptional activator, but also mediates repression of a set of target genes (PubMed:17984056, PubMed:26951377, PubMed:27378237, PubMed:28459454, PubMed:28459457, PubMed:29572508, PubMed:29618456, PubMed:30540931). Promotes expression of ZSCAN4 and KDM4E, two proteins with essential roles during early embryogenesis (PubMed:26951377, PubMed:27378237, PubMed:28459457, PubMed:29618456). Promotes nuclear translocation of CTNNB1/beta-catenin and its subsequent activation of target genes (PubMed:36158201). Heterologous expression in cultured embryonic stem cells mediates transcription of HERVL retrotransposons and transcripts derived from ACRO1 and HSATII satellite repeats (PubMed:28459457). May activate expression of PITX1 (PubMed:17984056). May regulate microRNA (miRNA) expression (PubMed:24145033). Inappropriate expression can inhibit myogenesis and promote apoptosis (PubMed:26951377, PubMed:28935672, PubMed:29618456). Isoform 2. Probably inactive as a transcriptional activator, due to the absence of the C-terminal region that is important for transcriptional activation. Can inhibit transcriptional activation mediated by isoform 1. Heterologous expression of isoform 2 has no deleterious effect on cell survival.

Alternative names

DUX10, DUX4, Double homeobox protein 4, Double homeobox protein 10

Recommended products

Rabbit Recombinant Monoclonal DUX4 antibody. Suitable for WB and reacts with Human samples. Cited in 34 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
E5-5
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

For details on the development of this clone, please refer to Geng et al. Hybridoma (Larchmt). 2011 Apr; 30(2): 125–130. PMID: 21529284

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Dux4 also known as Double homeobox 4 is a transcription factor with an important role in gene regulation. It has a molecular mass of approximately 52 kDa and is expressed mainly in germline and early embryonic tissues but it is usually silenced in adults. Dux4 contains a double homeobox domain which facilitates its ability to bind DNA sequences and regulate target gene expression. Despite its limited expression in adult tissues Dux4 is present in low levels in certain muscle tissues.

Biological function summary

Dux4 regulates a wide range of genes associated with muscle differentiation and apoptosis. It is not typically part of a known protein complex but acts independently to influence gene activity. Its function as a transcription factor impacts muscle cell differentiation by activating various myogenic and apoptotic genes. This regulatory activity plays a major role in normal muscle function and development in embryos.

Pathways

Dux4 plays a significant role in the Wnt signaling and beta-catenin pathways. These pathways are important for tissue development and cellular proliferation. Dux4 interacts with proteins like beta-catenin to influence cell growth and differentiation. Inappropriate activation of these pathways due to Dux4 involvement can result in dysregulated cell growth impacting muscle tissue integrity.

Associated diseases and disorders

The inappropriate expression of Dux4 is related to facioscapulohumeral muscular dystrophy (FSHD) and cancer. FSHD involves the misexpression of Dux4 in muscle tissues leading to muscle weakness and degeneration. In cancer Dux4 misexpression can contribute to tumor development and progression by disrupting normal cell differentiation processes. Dux4's interaction with proteins involved in these conditions such as beta-catenin helps to elucidate its role in disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Dux4 antibody [E5-5] (ab124699), expandable thumbnail

    Western blot - Anti-Dux4 antibody [E5-5] (ab124699)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

    Lane 1: HT1080 (human fibrosarcoma cell line) whole cell lysate at 20 µg

    Lane 2: PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 3: Saos-2 (human osteosarcoma cell line) whole cell lysate at 20 µg

    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

  • Western blot - Anti-Dux4 antibody [E5-5] (ab124699), expandable thumbnail

    Western blot - Anti-Dux4 antibody [E5-5] (ab124699)

    All lanes: Western blot - Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

    Lane 1: Human testis lysate at 10 µg

    Lane 2: HT1080 (human fibrosarcoma cell line) cell lysate at 10 µg

    Lane 3: PC-3 (human prostate adenocarcinoma cell line) cell lysate at 10 µg

    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 45 kDa, 52 kDa

  • Western blot - Anti-Dux4 antibody [E5-5] (ab124699), expandable thumbnail

    Western blot - Anti-Dux4 antibody [E5-5] (ab124699)

    Blocking buffer and concentration : 5% NFDM/TBST
    Diluting buffer and concentration : 5% NFDM /TBST
    The expression profile observed in HFF-1 is consistent with the literature (PMID: 30420784).
    Negative control : HFF-1 (PMID: 30420784)

    Lanes 1 - 5: Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (Anti-Dux4 antibody [E5-5] - BSA and Azide free ab216893) at 1/1000 dilution

    Lanes 1 - 5: Western blot - Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: HFF-1 (Human Skin fibroblast) whole cell lysate at 20 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

    Exposure time: 60s

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Product protocols

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