AB216893

Anti-Dux4 antibody [E5-5] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal DUX4 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DUX10, DUX4, Double homeobox protein 4, Double homeobox protein 10

3 Images

Lab

Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (AB216893)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM /TBST The expression profile observed in HFF-1 is consistent with the literature (PMID : 30420784). Negative control : HFF-1 (PMID : 30420784) This data was developed using ab124699, the same antibody clone in a different buffer formulation.

Lanes 1 - 5:

Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (ab216893) at 1/1000 dilution

Lanes 1 - 5:

Western blot - Anti-Dux4 antibody [E5-5] (<a href='/en-us/products/primary-antibodies/dux4-antibody-e5-5-ab124699'>ab124699</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

HFF-1 (Human Skin fibroblast) whole cell lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Exposure time: 60s

Unknown

Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (AB216893)

This data was developed using ab124699, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Dux4 antibody [E5-5] (<a href='/en-us/products/primary-antibodies/dux4-antibody-e5-5-ab124699'>ab124699</a>) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

HT1080 (human fibrosarcoma cell line) cell lysate at 10 µg

Lane 3:

PC-3 (human prostate adenocarcinoma cell line) cell lysate at 10 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 45 kDa,52 kDa

false

Lab

Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (AB216893)

This data was developed using ab124699, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dux4 antibody [E5-5] (<a href='/en-us/products/primary-antibodies/dux4-antibody-e5-5-ab124699'>ab124699</a>) at 1/1000 dilution

Lane 1:

HT1080 (human fibrosarcoma cell line) whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

Saos-2 (human osteosarcoma cell line) whole cell lysate at 20 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E5-5

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab216893 is the carrier-free version of ab124699.

For details on the developement of this clone, please refer to Geng et al. Hybridoma (Larchmt). 2011 Apr; 30(2): 125–130. PMID: 21529284

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dux4 also known as Double homeobox 4 is a transcription factor with an important role in gene regulation. It has a molecular mass of approximately 52 kDa and is expressed mainly in germline and early embryonic tissues but it is usually silenced in adults. Dux4 contains a double homeobox domain which facilitates its ability to bind DNA sequences and regulate target gene expression. Despite its limited expression in adult tissues Dux4 is present in low levels in certain muscle tissues.

Biological function summary

Dux4 regulates a wide range of genes associated with muscle differentiation and apoptosis. It is not typically part of a known protein complex but acts independently to influence gene activity. Its function as a transcription factor impacts muscle cell differentiation by activating various myogenic and apoptotic genes. This regulatory activity plays a major role in normal muscle function and development in embryos.

Pathways

Dux4 plays a significant role in the Wnt signaling and beta-catenin pathways. These pathways are important for tissue development and cellular proliferation. Dux4 interacts with proteins like beta-catenin to influence cell growth and differentiation. Inappropriate activation of these pathways due to Dux4 involvement can result in dysregulated cell growth impacting muscle tissue integrity.

The inappropriate expression of Dux4 is related to facioscapulohumeral muscular dystrophy (FSHD) and cancer. FSHD involves the misexpression of Dux4 in muscle tissues leading to muscle weakness and degeneration. In cancer Dux4 misexpression can contribute to tumor development and progression by disrupting normal cell differentiation processes. Dux4's interaction with proteins involved in these conditions such as beta-catenin helps to elucidate its role in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Transcription factor that is selectively and transiently expressed in cleavage-stage embryos (PubMed : 28459457). Binds to double-stranded DNA elements with the consensus sequence 5'-TAATCTAATCA-3' (PubMed : 28459454, PubMed : 28459457, PubMed : 29572508, PubMed : 30315230, PubMed : 30540931). Binds to chromatin containing histone H3 acetylated at 'Lys-27' (H3K27ac) and promotes deacetylation of H3K27ac. In parallel, binds to chromatin that lacks histone H3 acetylation at 'Lys-27' (H3K27ac) and recruits EP300 and CREBBP to promote acetylation of histone H3 at 'Lys-27' at new sites (PubMed : 26951377). Involved in transcriptional regulation of numerous genes, primarily as transcriptional activator, but also mediates repression of a set of target genes (PubMed : 17984056, PubMed : 26951377, PubMed : 27378237, PubMed : 28459454, PubMed : 28459457, PubMed : 29572508, PubMed : 29618456, PubMed : 30540931). Promotes expression of ZSCAN4 and KDM4E, two proteins with essential roles during early embryogenesis (PubMed : 26951377, PubMed : 27378237, PubMed : 28459457, PubMed : 29618456). Promotes nuclear translocation of CTNNB1/beta-catenin and its subsequent activation of target genes (PubMed : 36158201). Heterologous expression in cultured embryonic stem cells mediates transcription of HERVL retrotransposons and transcripts derived from ACRO1 and HSATII satellite repeats (PubMed : 28459457). May activate expression of PITX1 (PubMed : 17984056). May regulate microRNA (miRNA) expression (PubMed : 24145033). Inappropriate expression can inhibit myogenesis and promote apoptosis (PubMed : 26951377, PubMed : 28935672, PubMed : 29618456).. Isoform 2. Probably inactive as a transcriptional activator, due to the absence of the C-terminal region that is important for transcriptional activation. Can inhibit transcriptional activation mediated by isoform 1. Heterologous expression of isoform 2 has no deleterious effect on cell survival.

See full target information DUX4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:179 PubMed40234972

2025

KLF6-mediated glutamine metabolism governs odontogenic differentiation and matrix mineralization of dental pulp stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhi Wu,Zekai Xu,Yulian Zhang,Xiatong Zhang,Xiaoyuan Huang,Zhijian Xie,Zhuo Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dux4 antibody [E5-5] - BSA and Azide free

Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (AB216893)

Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (AB216893)

Western blot - Anti-Dux4 antibody [E5-5] - BSA and Azide free (AB216893)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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