AB117512

DyLight® 488 Anti-6X His tag® antibody [AD1.1.10]

Name: DyLight® 488 conjugated Anti-6X His tag® antibody [AD1.1.10] (ab117512) | Abcam
Brand: Abcam Limited
SKU: AB117512

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(10 Publications)

Anti-6X His tag® antibody [AD1.1.10] - DyLight® 488 conjugated (ab117512) is a mouse monoclonal antibody detecting 6X His tag® in Flow Cytometry, ICC/IF.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

Unconjugated

Anti-6X His tag® antibody [AD1.1.10]
657 SureLight® APC

SureLight® APC Anti-6X His tag® antibody [AD1.1.10]
Alkaline Phosphatase

Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10]
576 DyLight® 550

DyLight® 550 Anti-6X His tag® antibody [AD1.1.10]
HRP

HRP Anti-6X His tag® antibody [AD1.1.10]
675 PerCP

PerCP Anti-6X His tag® antibody [AD1.1.10]
578 PE

PE Anti-6X His tag® antibody [AD1.1.10]
673 DyLight® 650

DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD1.1.10

Isotype

IgG1

Conjugation

DyLight® 488

Excitation/Emission

Ex: 493nm, Em: 518nm

Carrier free

Applications

Flow Cyt, ICC/IF

applications

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Reactivity data

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Product details

What is this antibody validated in?
DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone AD1.1.10 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® DyLight® 488 [AD1.1.10] (ab117512).

Other related products
We have a range of other formats of antibody clone [AD1.1.10] also available for your convenience: ab15149, PE - ab72467, SureLight® APC - ab72579, Alkaline Phosphatase - ab81652, PerCP - ab117496, Carrier free - ab117504, DyLight® 550 - ab117507, DyLight® 488 - ab117512, HRP - ab178563, Carrier free - ab307236

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 80.5% Disodium hydrogenorthophosphate, 18.5% Sodium dihydrogen phosphate, 0.88% Sodium chloride, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7889 PubMed40849430

2025

Mesothelin-directed protein-drug conjugates for mesothelin-low solid tumor therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Jiayao Yan,Lin Li,Tianran Chen,Jingyi Guo,Lei Lei,Siwen Wu,Lijing Zhu,Xiaotong Chen,Baorui Liu

Protein science : a publication of the Protein Society 34:e70263 PubMed40815278

2025

Computational modeling and experimental validation of the interaction between tumor biomarker mesothelin and an engineered targeting protein with therapeutic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Piccardi,Valeria Butera,Ignazio Sardo,Stefano Landi,Federica Gemignani,Giampaolo Barone,Angelo Spinello,Sarah J Moore

Theranostics 14:6249-6267 PubMed39431011

2024

Development of a CD39 nanobody and its enhancement to chimeric antigen receptor T cells efficacy against ovarian cancer in preclinical studies.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chen Zhang,Xian-Yang Li,Qi Deng,Yan-Jun Ge,Rui-Rong Yi,Hua-Jing Wang,Jian-Tao Wang,Hui Zhou,Xue-Feng Kong,Rong-Jiao Liu,Yu-Ting Zhang,Xiao-Pei Li,Xiao-Wen He,Hai-Yan Zhu

Applied microbiology and biotechnology 107:1959-1970 PubMed36729226

2023

Production and applications of fluorobody from redox-engineered Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Witsanu Srila,Thae Thae Min,Thitima Sumphanapai,Kuntalee Rangnoi,Mehmet Berkmen,Montarop Yamabhai

Medical oncology (Northwood, London, England) 39:205 PubMed36175701

2022

Targeting acute myeloid cell surface using a recombinant antibody isolated from whole-cell biopanning of a phage display human scFv antibody library.

Applications

Unspecified application

Species

Unspecified reactive species

Thitima Sumphanapai,Kerry Chester,Surasak Sawatnatee,Jenny Yeung,Montarop Yamabhai

ACS omega 6:762-774 PubMed33458528

2021

Human Hexa-Histidine-Tagged Single-Chain Variable Fragments for Bioimaging of Bacterial Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Thae Thae Min,Montarop Yamabhai

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine 39:761-773 PubMed31702068

2019

Optimized Anti-Prostate-Specific Membrane Antigen Single-Chain Variable Fragment-Loaded Nanobubbles as a Novel Targeted Ultrasound Contrast Agent for the Diagnosis of Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Ding,Qifeng Cao,Subo Qian,Xiaolong Chen,Yuhong Xu,Jian Chen,Haibo Shen

Biotechnology and bioengineering 117:330-341 PubMed31631324

2019

Engineered Fn3 protein has targeted therapeutic effect on mesothelin-expressing cancer cells and increases tumor cell sensitivity to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Allison R Sirois,Daniela A Deny,Yanxuan Li,Yacine D Fall,Sarah J Moore

Proceedings of the National Academy of Sciences of 113:E4847-56 PubMed27482114

2016

CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Beat A Imhof,Stephane Jemelin,Romain Ballet,Christian Vesin,Marc Schapira,Melis Karaca,Yalin Emre

PloS one 9:e107521 PubMed25285446

2014

Expression cloning of camelid nanobodies specific for Xenopus embryonic antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Keiji Itoh,Sergei Y Sokol

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

DyLight® 488 Anti-6X His tag® antibody [AD1.1.10]

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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